Genetic diversity analysis based on ISSR molecular markers in <i>Spathiphyllum</i>

GUO Tianxin; Muhammad Moaaz Ali; YAN Xixi; LI Xue; JIAN Liguan; PENG Jinbin; CHEN Xinyan; ZHAO Jinhe; PENG Junxian; CHEN Faxing

doi:10.19303/j.issn.1008-0384.2022.005.011

Volume 37 Issue 5

May 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(5): 632-640

GUO T X, MUHAMMAD M A, YAN X X, et al. Genetic diversity analysis based on ISSR molecular markers in Spathiphyllum [J]. Fujian Journal of Agricultural Sciences，2022，37（5）：632−640 doi: 10.19303/j.issn.1008-0384.2022.005.011

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GUO T X, MUHAMMAD M A, YAN X X, et al. Genetic diversity analysis based on ISSR molecular markers in Spathiphyllum [J]. Fujian Journal of Agricultural Sciences，2022，37（5）：632−640 doi: 10.19303/j.issn.1008-0384.2022.005.011

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Genetic diversity analysis based on ISSR molecular markers in Spathiphyllum

doi: 10.19303/j.issn.1008-0384.2022.005.011

1.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Quanmei Biotechnology Company of Quanzhou City, Quanzhou, Fujian　362000, China
3.
Luojiang District Forestry Bureau Technology Extension Station of Quanzhou , Quanzhou Fujian　362000, China
4.
Municipal Public Utilities Management Center, Quanzhou Luojiang District , Quanzhou, Fujiian　362000, China
5.
Forestry Bureau of Anxi County, Quanzhou, Fujian　362400, China
6.
Luojiang District Natural Resources Bureau of Quanzhou City, Quanzhou, Fujian　362000, China

Received Date: 2022-03-04
Rev Recd Date: 2022-05-26

Available Online: 2022-06-20

Publish Date: 2022-05-28

Abstract

Abstract

Objective In order to provide theoretical basis for the development, utilization and protection of Spathiphyllum germplasm resources, the genetic diversity and relationship of germplasm resources were discussed. Method ISSR markers were used to analyze the genetic relationship and genetic diversity of 21 Spathiphyllum germplasm resources from 14 cultivars and 7 hybrid strains. Result Nine clearly amplified and highly polymorphic ISSR primers were used for PCR amplification of 21 germplasm. A total of 133 loci were amplified, including 120 polymorphic loci, and the polymorphism ratio (PPB) was 90.23%. According to NTsys cluster analysis, the 14 cultivars could be divided into three groups at the similarity coefficient of 0.6692. The 7 hybrid strains could be divided into two groups at the similarity coefficient of 0.6700. Using TBtools correlation and principal component analysis, 14 cultivars were divided into two groups on the first principal coordinate, which proved the accuracy of cluster analysis results. Conclusion ISSR molecular markers could effectively reveal the genetic diversity and the genetic relationship between cultivars; the genetic diversity of the spathiphyllum is abundant. ISSR clustering results were in good agreement with the germplasm origin and the character of spathes; principal component analysis confirmed the clustering results and showed the genetic relationship between varieties more directly.
- Spathiphyllum,
- germplasm resources,
- ISSR marker,
- genetic diversity

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References(24)

References

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