Biological Characteristics and Fungicides of Cherry Leaf Spot Disease Pathogen

WANG Lei; ZHI Huanhuan; MA Yongqiang; YAO Qiang; CHEN Hongyu; ZHANG Gang; GUO Qingyun

doi:10.19303/j.issn.1008-0384.2022.004.011

Volume 37 Issue 4

Apr. 2022

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(4): 503-513

WANG L, ZHI H H, MA Y Q, et al. Biological Characteristics and Fungicides of Cherry Leaf Spot Disease Pathogen [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：503−513 doi: 10.19303/j.issn.1008-0384.2022.004.011

Citation:

WANG L, ZHI H H, MA Y Q, et al. Biological Characteristics and Fungicides of Cherry Leaf Spot Disease Pathogen [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：503−513 doi: 10.19303/j.issn.1008-0384.2022.004.011

Citation:

PDF( 1202 KB)

Biological Characteristics and Fungicides of Cherry Leaf Spot Disease Pathogen

doi: 10.19303/j.issn.1008-0384.2022.004.011

1.
Academy of Agriculture and Forestry Sciences, Qinghai University/Key Laboratory of Agriculture Pest Management, Institute of Plant Protection, Scientific Observation and experiment Station of Crop Pest in Xining, Ministry of Agriculture, Xining, Qinghai　810016, China
2.
Agricultural Technology Extension Center, Haidong, Qinghai　810600, China

Received Date: 2021-12-23
Rev Recd Date: 2022-02-03

Available Online: 2022-03-21

Publish Date: 2022-04-28

Abstract

Abstract

Objective Biological characteristics of Colletotrichum salicis, the pathogen that causes the leaf spot disease on cherry plants in Qinghai Province, were studied and effective fungicides evaluated for the control. Methods C. salicis were examined by the cross and spore counting methods. Its sensitivity to 26 fungicides was determined in the laboratory according to the growth inhibition on culture media. Results PDA medium was found to be optimal for the C. salicis mycelial growth and sporulation. Inositol and glucose were the optimal carbon sources for sporulation, while beef extract the nitrogen for colony growth and peptone for spore production. The pathogen could grow between 5 ℃ and 40 ℃ but most rapidly at 25 ℃ and died at 58 ℃ in 10 min; between pH 4 and 12 but optimally at pH 7; and well under a photoperiod cycle of 12 h light/12 h dark. Among the tested antifungal agents, 10% phentermine methiconazole aqueous dispersion or 0.3% eugenol dissolvable solution had the greatest inhibition with EC₅₀ of 0.6 mg·L⁻¹ and 1.151 mg·L⁻¹, respectively, whereas 20% allicin oil emulsion, 80% manganese zinc wettable powder, and 500 g·L⁻¹ isobaric urea suspension the least efficacies with EC₅₀ up to 301.44 mg·L⁻¹, 679.36 mg·L⁻¹, and 1 012.52 mg·L⁻¹, respectively. Conclusion The conditions and nutrients for optimal C. salicis culture were determined and the effective fungicides were identified.
- Cherry leaf spot,
- Colletotrichum salicis,
- biological characteristics,
- highly effective fungicides,
- virulence assay

FullText(HTML)

References(39)

References

[1]	孙杨, 付全娟, 孙玉刚, 等. 樱桃褐斑病病原菌生物学特性及品种抗性评价 [J]. 植物保护, 2017, 43(4):110−114. doi: 10.3969/j.issn.0529-1542.2017.04.020 SUN Y, FU Q J, SUN Y G, et al. Identification of cherry cultivar resistance to leaf spot and biological characteristics of Passalora circumscissa [J]. Plant Protection, 2017, 43(4): 110−114.(in Chinese) doi: 10.3969/j.issn.0529-1542.2017.04.020
[2]	MCCUNE L M, KUBOTA C, STENDELL-HOLLIS N R, et al. Cherries and health: A review [J]. Critical Reviews in Food Science and Nutrition, 2011, 51(1): 1−12.
[3]	王琴, 王建友, 韩宏伟, 等. 南疆地区甜樱桃品种果实品质测定与评价 [J]. 食品工业科技, 2019, 40(8):215−220. WANG Q, WANG J Y, HAN H W, et al. Determination and evaluation of fruit quality of sweet cherry cultivars in southern Xinjiang [J]. Science and Technology of Food Industry, 2019, 40(8): 215−220.(in Chinese)
[4]	黄贞光, 刘聪利, 李明, 等. 近20年国内外甜樱桃产业发展动态及对未来的预测 [J]. 果树学报, 2014(S1):1−6. HUANG Z G, LIU C L, LI M, et al. The development situation of sweet cherry industry in China and abroad during recent two decates and prognostication for the future [J]. Journal of Fruit Science, 2014(S1): 1−6.(in Chinese)
[5]	张艳婷, 仇智灵, 李阿根, 等. 浙江省樱桃褐腐病病原菌种类及其对常见药剂的抗性检测 [J]. 果树学报, 2020, 37(9):1394−1403. ZHANG Y T, QIU Z L, LI A G, et al. Species of pathogens causing cherry brown rot and their resistance to common fungicides in Zhejiang Province [J]. Journal of Fruit Science, 2020, 37(9): 1394−1403.(in Chinese)
[6]	徐丽, 王甲威, 陈新, 等. 甜樱桃流胶病原菌的分子鉴定和致病性检测 [J]. 植物病理学报, 2015, 45(4):350−355. XU L, WANG J W, CHEN X, et al. Identification and pathogenicity detection of the cherry gummosis pathogen [J]. Acta Phytopathologica Sinica, 2015, 45(4): 350−355.(in Chinese)
[7]	王娟, 邓朋, 李中学, 等. 5种樱桃果病害的发生规律与防治 [J]. 现代园艺, 2018(19):159−160. doi: 10.3969/j.issn.1006-4958.2018.19.085 WANG J, DENG P, LI Z X, et al. Occurrence regularity and control of five cherry fruit diseases [J]. Xiandai Horticulture, 2018(19): 159−160.(in Chinese) doi: 10.3969/j.issn.1006-4958.2018.19.085
[8]	LOPEZ-CARBONELL M, MORET A, NADAL M. Changes in cell ultrastructure and zeatin riboside concentrations in Hedera helix, Pelargonium zonale, Prunus avium, and Rubus ulmifolius leaves infected by fungi [J]. Plant Disease, 1998, 82(8): 914−918. doi: 10.1094/PDIS.1998.82.8.914
[9]	刘俏. 青海省樱桃叶斑病病原种类鉴定及防治药剂室内筛选研究[D]. 西宁: 青海大学, 2020. LIU Q. Identification of the pathogens causing sweet cherry leaf spot in Qinghai province and screening fungicides in laboratory [D]. Xining: Qinghai University, 2020. (in Chinese)
[10]	刘一贤, 蔡志英, 施玉萍, 等. 辣木果腐病病原菌兰生炭疽菌(Colletotrichum chlorophyti)生物学特性及其防治药剂室内毒力测定 [J]. 江苏农业科学, 2019, 47(20):133−137. LIU Y X, CAI Z Y, SHI Y P, et al. Biological characteristics of Colletotrichum chlorophyti, a pathogen of moringa oleifera fruit rot disease, and laboratory virulence determination of its control agents [J]. Jiangsu Agricultural Sciences, 2019, 47(20): 133−137.(in Chinese)
[11]	李文, 何月秋, 王佳莹, 等. 百日草炭疽病病原菌的分离鉴定及防治药剂筛选 [J]. 农药学学报, 2021, 23(2):341−347. LI W, HE Y Q, WANG J Y, et al. Isolation and identification of pathogen causing anthracnose on Zinnia elegans Jacq. and fungicides screening [J]. Chinese Journal of Pesticide Science, 2021, 23(2): 341−347.(in Chinese)
[12]	张琳, 占浩鑫, 冯志伟, 等. 人参生炭疽菌Colletotrichum panacicola和线列炭疽菌C. lineola的生物学特性及其对不同杀菌剂的敏感性研究[J/OL]. 植物病理学报: 1-14[2021-10-22]. Doi: 10.13926/j.cnki.apps.000769. ZHANG L, ZHAN H X, FENG Z W, et al. Biological characteristics and fungicide sensitivity of Colletotrichum panacicola and C. lineola causing anthracnose on ginseng [J/OL]. Acta Phytopathologica Sinica: 1-14. [2021-10-22]. Doi: 10.13926/j.cnki.apps.000769 (in Chinese).
[13]	邵慧慧, 张西梅, 刘紫祺, 等. 引起西洋参锈腐病的Ilyonectria属4种病原菌的生物学特性及其对不同杀菌剂的敏感性[J/OL]. 植物病理学报: 1-11[2021-09-16]. Doi: 10.13926/j.cnki.apps.000746. SHAO H H, ZHANG X M, LIU Z Q, et al. Biological characteristics and fungicide sensitivity of four Ilyonectria species causing root rot on American ginseng [J/OL]. Acta Phytopathologica Sinica: 1-11. [2021-09-16]. Doi: 10.13926/j.cnki.apps.000746 (in Chinese).
[14]	张秀伟, 蔡甫格, 潘中涛, 等. 金刺梨黑斑病病原菌生物学特性及室内药剂毒力测定 [J]. 江苏农业科学, 2018, 46(18):98−100. ZHANG X W, CAI F G, PAN Z T, et al. Biological characteristics of the pathogenic bacteria of rosa roxburghii black spot disease and determination of indoor toxicity [J]. Jiangsu Agricultural Sciences, 2018, 46(18): 98−100.(in Chinese)
[15]	吴松, 陈全助, 张晓阳, 等. 樟树溃疡病原菌生物学特性及室内毒力测定 [J]. 森林与环境学报, 2021, 41(3):308−317. WU S, CHEN Q Z, ZHANG X Y, et al. Studies on biological characteristics of a Camphor tree canker pathogen (Neofusicoccum Parvum) and fungicide laboratory toxicity [J]. Journal of Forest and Environment, 2021, 41(3): 308−317.(in Chinese)
[16]	郭宁, 胡清玉, 刘粤阳, 等. 玉米叶斑病菌麦根腐平脐蠕孢的生物学特性及其对杀菌剂的敏感性 [J]. 华北农学报, 2019, 34(S1):289−295. doi: 10.7668/hbnxb.20190188 GUO N, HU Q Y, LIU Y Y, et al. Biological characteristics and sensitivity to fungicides of Bipolaris sorokiniana causing maize leaf spot [J]. Acta Agriculturae Boreali-Sinica, 2019, 34(S1): 289−295.(in Chinese) doi: 10.7668/hbnxb.20190188
[17]	范文忠, 王振, 顾园园, 等. 水蜡叶斑病病原菌鉴定及对药剂的敏感性 [J]. 农药, 2020, 59(1):60−64. FAN W Z, WANG Z, GU Y Y, et al. Pathogen identification of Ligustrum obtusifolium leaf spot and fungicides susceptibility [J]. Agrochemicals, 2020, 59(1): 60−64.(in Chinese)
[18]	孔琼, 袁盛勇, 郭建伟, 等. 野蚕豆根根腐病原菌、生物学特性及其有效杀菌剂研究 [J]. 西南农业学报, 2020(7):1480−1485. KONG Q, YUAN S Y, GUO J W, et al. Identification, biological characteristics and fungicides screening of pathogen on root-rot disease in Centranthera grandiflora [J]. Southwest China Journal of Agricultural Sciences, 2020(7): 1480−1485.(in Chinese)
[19]	孙宇, 白庆荣. 吉林省水稻鞘枯病病菌生物学特性及药剂敏感性 [J]. 农药, 2018, 57(10):757−761,767. SUN Y, BAI Q R. Pathogen identification and biological characteristics of rice sheath blight caused by Nigrospora oryzae in Jilin Province and susceptibility to fungicides [J]. Agrochemicals, 2018, 57(10): 757−761,767.(in Chinese)
[20]	田华, 朱艳梅, 董瑜, 等. 烟草麻孢根腐病菌生理学特性及药剂毒力测定 [J]. 植物保护学报, 2017, 44(3):488−494. TIAN H, ZHU Y M, DONG Y, et al. Physiological characteristics of Gelasinospora reticulata and toxicity test of 11 fungicides [J]. Journal of Plant Protection, 2017, 44(3): 488−494.(in Chinese)
[21]	魏蜜, 朱洁倩, 张伟, 等. 玛咖根腐病菌的生物学特性及防治药剂室内筛选 [J]. 云南农业大学学报(自然科学版), 2017, 32(5):787−792. WEI M, ZHU J Q, ZHANG W, et al. Biological characteristics of pathogen causing maca root rot disease and its fungicides laboratory screening [J]. Journal of Yunnan Agricultural University(Natural Science Edition), 2017, 32(5): 787−792.(in Chinese)
[22]	常佳迎, 田兰芝, 刘树森, 等. 新月弯孢变种的生物学特性及其对药剂的敏感性 [J]. 植物保护学报, 2020, 47(5):1038−1047. CHANG J Y, TIAN L Z, LIU S S, et al. Biological characteristics of fungal pathogen Curvularia lunata varieties and its sensitivity to fungicides [J]. Journal of Plant Protection, 2020, 47(5): 1038−1047.(in Chinese)
[23]	王春伟, 王燕, 张曦倩, 等. 越橘镰孢果腐病菌的生物学特性测定及防治药剂初步筛选 [J]. 园艺学报, 2017, 44(8):1589−1598. WANG C W, WANG Y, ZHANG X Q, et al. Determination of biological characteristics and preliminary screening of control fungicides of Fusarium acuminatum causing Fusarium fruit rot on blueberry [J]. Acta Horticulturae Sinica, 2017, 44(8): 1589−1598.(in Chinese)
[24]	李润根, 卢其能, 何咪, 等. 百合新病原菌假短孢弯孢生物学特性及其对杀菌剂的敏感性 [J]. 植物保护, 2020, 46(6):41−46. LI R G, LU Q N, HE M, et al. The biological characteristics of Curvularia pseudobrachyspora, a new causal agent of lily leaf spot, and its sensitivity to fungicides [J]. Plant Protection, 2020, 46(6): 41−46.(in Chinese)
[25]	陈宏州, 杨敬辉, 肖婷, 等. 12种杀菌剂对葡萄灰霉病菌的毒力测定 [J]. 江苏农业科学, 2015, 43(1):124−126,127. CHEN H Z, YANG J H, XIAO T, et al. Determination of virulence of 12 fungicides against Botrytis cinerea [J]. Jiangsu Agricultural Sciences, 2015, 43(1): 124−126,127.(in Chinese)
[26]	刘丽萍, 高洁, 李玉. 植物炭疽菌属Colletotrichum真菌研究进展 [J]. 菌物研究, 2020, 18(4):266−281. LIU L P, GAO J, LI Y. Advances in knowledge of the fungi referred to the genus Colletotrichum [J]. Journal of Fungal Research, 2020, 18(4): 266−281.(in Chinese)
[27]	向梅梅, 张云霞, 刘霄. 炭疽菌属真菌分类的研究进展 [J]. 仲恺农业工程学院学报, 2017, 30(1):59−66. doi: 10.3969/j.issn.1674-5663.2017.01.012 XIANG M M, ZHANG Y X, LIU X. Research progress on the taxonomy of Colletotrichum [J]. Journal of ZhongKai University of Agriculture and Technology, 2017, 30(1): 59−66.(in Chinese) doi: 10.3969/j.issn.1674-5663.2017.01.012
[28]	KOELSCH M C, COLE J C, VONBROEMBSEN S L. First report of leaf spots and stem lesions on common periwinkle caused by Colletotrichum gloeosporioides [J]. Plant Disease, 1995, 79(1): 83.
[29]	GAUTAM A K, AVASTHI S, BHADAURIA R. First report of anthracnose caused by Colletotrichum gloeosporioides on Boehravia diffusa in India [J]. Archives of Phytopathology and Plant Protection, 2012, 45(20): 2502−2506. doi: 10.1080/03235408.2012.730888
[30]	BI Y, GUO W, ZHANG G J, et al. First report of Colletotrichum boninense causing anthracnose of strawberry in China (Article) [J]. Plant Disease, 2017, 101(1): 250−251.
[31]	OKORSKI A, PSZCZOLKOWSKA A, SULIMA P, et al. First report of willow anthracnose caused by Colletotrichum salicis in Poland (Note) [J]. Plant Disease, 2018, 102(10): 2036−2041.
[32]	KARAMNEJADI T, SOLHIZADEH A, SHENAVAR O, et al. First report of leaf spot caused by Colletotrichum coccodes on viola tricolor in Iran (Note) [J]. Journal of Plant Pathology, 2017, 99(2): 536.
[33]	孙洁, 池振江, 赵思峰. 新疆红枣缩果病菌生物学特性及室内药剂筛选研究 [J]. 北方园艺, 2013(24):126−129. SUN J, CHI Z J, ZHAO S F. Study on biological characteristics of Alternaria alternata caused jujube shrink disease and screening of fungicides in Xinjiang [J]. Northern Horticulture, 2013(24): 126−129.(in Chinese)
[34]	杨佳文, 赵尊练, 张管曲, 等. 陕西线辣椒炭疽病原菌的鉴定及生物学特性研究 [J]. 西北农业学报, 2017, 26(11):1695−1705. doi: 10.7606/j.issn.1004-1389.2017.11.017 YANG J W, ZHAO Z L, ZHANG G Q, et al. Identification and biological characterization of Anthrax bacteria in xianlajiao chili pepper in Shaanxi Province [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2017, 26(11): 1695−1705.(in Chinese) doi: 10.7606/j.issn.1004-1389.2017.11.017
[35]	秦健, 黄如葵, 黄熊娟, 等. 苦瓜双色平脐蠕孢叶斑病病原菌的生物学特性及其抑菌药剂筛选 [J]. 热带作物学报, 2020, 41(12):2507−2512. doi: 10.3969/j.issn.1000-2561.2020.12.019 QIN J, HUANG R K, HUANG X J, et al. Biological characteristics of Momordica charantia leaf spot pathogen Bipolaris bicolor and bacteriostatic agents screening [J]. Chinese Journal of Tropical Crops, 2020, 41(12): 2507−2512.(in Chinese) doi: 10.3969/j.issn.1000-2561.2020.12.019
[36]	冯思琪, 张亚玲. 黑龙江水稻胡麻叶斑病病原菌的分离鉴定及生物学特性 [J]. 中国植保导刊, 2019, 39(2):17−23. doi: 10.3969/j.issn.1672-6820.2019.02.004 FENG S Q, ZHANG Y L. Pathogen isolation and identification of rice brown spot and its biological characteristics in Heilongjiang [J]. China Plant Protection, 2019, 39(2): 17−23.(in Chinese) doi: 10.3969/j.issn.1672-6820.2019.02.004
[37]	姜苏月, 靳俊媛, 刘静, 等. 羽扇豆炭疽病病原鉴定及室内药剂筛选 [J]. 东北林业大学学报, 2021, 49(5):136−141. doi: 10.3969/j.issn.1000-5382.2021.05.024 JIANG S Y, JIN J Y, LIU J, et al. Pathogen identification and screening of fungicides of anthracnose of Lupinus micranthus by Colletotrichum Lupi-ni [J]. Journal of Northeast Forestry University, 2021, 49(5): 136−141.(in Chinese) doi: 10.3969/j.issn.1000-5382.2021.05.024
[38]	邓洁, 莫飞旭, 石金巧, 等. 钩藤炭疽病病原鉴定、生物学特性及防治药剂筛选 [J]. 中药材, 2020, 43(6):1303−1307. DENG J, MO F X, SHI J Q, et al. Pathogen identification, biological characteristics of anthracnose in Uncaria hirsuta and screening of control fungicides [J]. Petroleum Geology and Recovery Efficiency, 2020, 43(6): 1303−1307.(in Chinese)
[39]	孟珂, 张亚波, 常君, 等. 8种杀菌剂对9种薄壳山核桃炭疽病病原菌的毒力测定 [J]. 林业科学研究, 2021, 34(1):153−164. MENG K, ZHANG Y B, CHANG J, et al. Toxicity Test with 8 Fungicides Against 9 Pathogens of Pecan Anthracnose (Colletotrichum spp. ) [J]. Forest Research, 2021, 34(1): 153−164.(in Chinese)