Bioinformatics and Expression of Hydroxycinnamyl transferase Gene Family from <i>Dendrobium officinale</i>

LONG Xiaoqin; NIE Cong; HE Jize; TIAN Ye; LI Xinran; PI Xinyao; WANG Wanjun; ZHU Qiankun

doi:10.19303/j.issn.1008-0384.2022.004.010

Volume 37 Issue 4

Apr. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(4): 492-502

LONG X Q, NIE C, HE J Z, et al. Bioinformatics and Expression of Hydroxycinnamyl transferase Gene Family from Dendrobium officinale [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：492−502 doi: 10.19303/j.issn.1008-0384.2022.004.010

Citation:

LONG X Q, NIE C, HE J Z, et al. Bioinformatics and Expression of Hydroxycinnamyl transferase Gene Family from Dendrobium officinale [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：492−502 doi: 10.19303/j.issn.1008-0384.2022.004.010

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Bioinformatics and Expression of Hydroxycinnamyl transferase Gene Family from Dendrobium officinale

doi: 10.19303/j.issn.1008-0384.2022.004.010

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan　610031, China

Received Date: 2021-10-26
Rev Recd Date: 2021-12-22

Available Online: 2022-06-19

Publish Date: 2022-04-28

Abstract

Abstract

Objective Characterization and the expressions of hydroxycinnamyl transferase (HCT) gene family from Dendrobium officinale were studied to reveal the biosynthesis of flavonoids and dendrophenol in the plant. Method Relevant gene sequences were obtained from the NCBI online website. Conserved motifs and domains, evolutionary tree, and protein secondary and tertiary structures of each member of DoHCTs were analyzed using bioinformatics methods. Expressions of the genes under stresses and in roots, stems, and leaves were determined using RT-PCR. Result The gene family had 8 members, DoHCT1–8. Their conserved domains showed that DoHCT7/8 belonged to the transferase superfamily and DoHCT1–6 to the PLN02481 superfamily. Abiotic stresses could affect the expressions, such as, elevated temperatures downregulated them. Except DoHCT7, the genes were differentially expressed in the roots, stems, and leaves. Conclusion The comprehensive bioinformatics and expression analyses on the 8 members of the DoHCT family from D. officinale revealed that SoHCTs were with differental expression under different abiotic stress or tissues, and proved to play regulatory role in abiotic stress.
- Dendrobium officinale,
- hydroxycinnamoyl transferase,
- expression analysis,
- flavonoid,
- dendrophenol

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References(23)

References

[1]	吴建, 王建方, 方玲, 等. 国内铁皮石斛研究概况 [J]. 中国药学杂志, 2013, 48(19):1610−1613. WU J, WANG J F, FANG L, et al. Domestic research progress of Dendrobium officinale kimura et migo [J]. Chinese Pharmaceutical Journal, 2013, 48(19): 1610−1613.(in Chinese)
[2]	王雨. 铁皮石斛的功能学和安全性研究进展 [J]. 微量元素与健康研究, 2021, 38(5):71−73. WANG Y. Research progress on function and safety of Dendrobium candidum [J]. Studies of Trace Elements and Health, 2021, 38(5): 71−73.(in Chinese)
[3]	XIAO J B, CAPANOGLU E, JASSBI A R, et al. Advance on the flavonoid C-glycosides and health benefits [J]. Critical Reviews in Food Science and Nutrition, 2016, 56(S1): S29−S45.
[4]	曾艺芸, 聂雪婷, 李振坚, 等. 中药石斛黄酮活性成分研究进展 [J]. 中国实验方剂学杂志, 2021, 27(6):197−206. ZENG Y Y, NIE X T, LI Z J, et al. Research progress on active ingredients of flavonoids in traditional Chinese medicine Dendrobium [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2021, 27(6): 197−206.(in Chinese)
[5]	刘昕皓, 魏粉菊, 王学顺, 等. 多酚类化合物的生物活性研究进展 [J]. 中国医药工业杂志, 2021, 52(4):471−483. LIU X H, WEI F J, WANG X S, et al. Progress on biological activities of polyphenols [J]. Chinese Journal of Pharmaceuticals, 2021, 52(4): 471−483.(in Chinese)
[6]	FRASER C M, CHAPPLE C. The phenylpropanoid pathway in Arabidopsis [J]. The Arabidopsis Book, 2011, 9: e0152. doi: 10.1199/tab.0152
[7]	KRIEGSHAUSER L, KNOSP S, GRIENENBERGER E, et al. Function of the hydroxycinnamoyl-coa: Shikimate hydroxycinnamoyl transferase is evolutionarily conserved in embryophytes [J]. The Plant Cell, 2021, 33(5): 1472−1491. doi: 10.1093/plcell/koab044
[8]	PENG H, MEYER R S, YANG T B, et al. A novel hydroxycinnamoyl transferase for synthesis of hydroxycinnamoyl spermine conjugates in plants [J]. BMC Plant Biology, 2019, 19(1): 261. doi: 10.1186/s12870-019-1846-3
[9]	ZHANG J R, WU M L, LI W D, et al. Regulation of chlorogenic acid biosynthesis by hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase in Lonicera japonica [J]. Plant Physiology and Biochemistry, 2017, 121: 74−79. doi: 10.1016/j.plaphy.2017.10.017
[10]	SULLIVAN M. A novel red clover hydroxycinnamoyl transferase has enzymatic activities consistent with a role in phaselic acid biosynthesis [J]. Plant Physiology, 2009, 150(4): 1866−1879. doi: 10.1104/pp.109.136689
[11]	SULLIVAN M L, ZARNOWSKI R. Red clover HCT2, a hydroxycinnamoyl-coenzyme A: Malate hydroxycinnamoyl transferase, plays a crucial role in biosynthesis of phaselic acid and other hydroxycinnamoyl-malate esters in vivo [J]. Plant Physiology, 2011, 155(3): 1060−1067. doi: 10.1104/pp.110.166793
[12]	刘菲, 刘辉, 梁元存, 等. 核黄素诱导烟草悬浮细胞酚类物质和木质素的积累 [J]. 中国农业科学, 2009, 42(9):3230−3235. doi: 10.3864/j.issn.0578-1752.2009.09.027 LIU F, LIU H, LIANG Y C, et al. Induction of phenolic compounds and lignin in cultured tobacco cells treated with riboflavin [J]. Scientia Agricultura Sinica, 2009, 42(9): 3230−3235.(in Chinese) doi: 10.3864/j.issn.0578-1752.2009.09.027
[13]	BESSEAU S, HOFFMANN L, GEOFFROY P, et al. Flavonoid accumulation in Arabidopsis repressed in lignin synthesis affects auxin transport and plant growth [J]. The Plant Cell, 2007, 19(1): 148−162. doi: 10.1105/tpc.106.044495
[14]	吕朝耕, 杨健, 康传志, 等. 铁皮石斛中10种黄酮类成分UPLC-MS/MS测定与多糖组成含量分析 [J]. 中国实验方剂学杂志, 2017, 23(17):47−52. LYU C G, YANG J, KANG C Z, et al. Determination of 10 flavonoids by UPLC-MS /MS and analysis of polysaccharide contents and compositions in dendrobii officinalis caulis from different habitats [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23(17): 47−52.(in Chinese)
[15]	林江波, 王伟英, 邹晖, 等. 基于转录组测序的铁皮石斛黄酮代谢途径及相关基因解析 [J]. 福建农业学报, 2019, 34(9):1019−1025. LIN J B, WANG W Y, ZOU H, et al. Transcriptome analysis on pathway of and genes related to flavonoid synthesis in Dendrobium officinale [J]. Fujian Journal of Agricultural Sciences, 2019, 34(9): 1019−1025.(in Chinese)
[16]	黄彪, 何伟, 吴建鸿, 等. UPLC-MS/MS同时测定铁皮石斛茎、叶、花中酚类组分的含量 [J]. 食品科学, 2021, 42(10):262−268. doi: 10.7506/spkx1002-6630-20200529-363 HUANG B, HE W, WU J H, et al. Simultaneous determination of phenolic components in Dendrobium officinale stems, leaves and flowers by ultra-high performance liquid chromatography-tandem mass spectrometry [J]. Food Science, 2021, 42(10): 262−268.(in Chinese) doi: 10.7506/spkx1002-6630-20200529-363
[17]	GROSS S R, KINZY T G. Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology [J]. Nature Structural & Molecular Biology, 2005, 12(9): 772−778.
[18]	严静, 蔡易熹, 陈燕兰, 等. 铁皮石斛茎、叶、花的活性成分及综合利用研究进展 [J]. 食品与发酵工业, 2021, 47(17):299−306. YAN J, CAI Y X, CHEN Y L, et al. Research progress in active components and comprehensive utilization of stems, leaves and flowers of Dendrobium officinale [J]. Food and Fermentation Industries, 2021, 47(17): 299−306.(in Chinese)
[19]	唐静月, 颜美秋, 齐芳芳, 等. 铁皮石斛花总黄酮提取工艺优化及体外抗氧化活性研究 [J]. 浙江中医药大学学报, 2017, 41(3):235−242. TANG J Y, YAN M Q, QI F F, et al. Study on optimum extraction of total flavones in Dendrobium officinale flower and its antioxidant activity in vitro [J]. Journal of Zhejiang Chinese Medical University, 2017, 41(3): 235−242.(in Chinese)
[20]	CHEN Y F, YI N, YAO S B, et al. Cs HCT-mediated lignin synthesis pathway involved in the response of tea plants to biotic and abiotic stresses [J]. Journal of Agricultural and Food Chemistry, 2021, 69(35): 10069−10081. doi: 10.1021/acs.jafc.1c02771
[21]	张新业, 王聪艳, 苏彦苹, 等. 菊芋木质素合成相关基因HtHCT的克隆及表达分析 [J]. 植物生理学报, 2020, 56(5):1023−1032. ZHANG X Y, WANG C Y, SU Y P, et al. Cloning and expression analysis of lignin biosynthesis related gene HtHCT from Helianthus tuberosus [J]. Plant Physiology Journal, 2020, 56(5): 1023−1032.(in Chinese)
[22]	董宏然, 杨健, 黄璐琦, 等. 菘蓝莽草酸羟基肉桂酸酰转移酶IiHCT基因的克隆和表达分析 [J]. 中国中药杂志, 2015, 40(21):4149−4154. DONG H R, YANG J, HUANG L Q, et al. Cloning and expression analysis of a hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferasegene(IiHCT) from Isatis indigotica [J]. China Journal of Chinese Materia Medica, 2015, 40(21): 4149−4154.(in Chinese)
[23]	CHAO N, QI Q, LI S, et al. Characterization and functional analysis of the Hydroxycinnamoyl-CoA: Shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar [J]. PeerJ, 2021, 9: e10741. doi: 10.7717/peerj.10741