Cloning and Expression of<i> NnWRKY</i>22 in Lotus

WANG Wanru; LIU Ying; LIU Hongli; HE Dan; LIU Yiping; KONG Dezheng

doi:10.19303/j.issn.1008-0384.2022.004.009

Volume 37 Issue 4

Apr. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(4): 486-491

WANG W R, LIU Y, LIU H L, et al. Cloning and Expression of NnWRKY22 in Lotus [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：486−491 doi: 10.19303/j.issn.1008-0384.2022.004.009

Citation:

WANG W R, LIU Y, LIU H L, et al. Cloning and Expression of NnWRKY22 in Lotus [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：486−491 doi: 10.19303/j.issn.1008-0384.2022.004.009

WANG W R, LIU Y, LIU H L, et al. Cloning and Expression of NnWRKY22 in Lotus [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：486−491 doi: 10.19303/j.issn.1008-0384.2022.004.009

Citation:

WANG W R, LIU Y, LIU H L, et al. Cloning and Expression of NnWRKY22 in Lotus [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：486−491 doi: 10.19303/j.issn.1008-0384.2022.004.009

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Cloning and Expression of NnWRKY22 in Lotus

doi: 10.19303/j.issn.1008-0384.2022.004.009

WANG Wanru^1
,,
LIU Ying¹,
LIU Hongli^{1, 2},
HE Dan^{1, 2},
LIU Yiping^{1, 2
,
,},
KONG Dezheng^{1, 2}

1.
Landscape Architecture and Art College, Henan Agricultural University, Zhengzhou, Henan　450002, China
2.
Henan Provincial Engineering Research Center of Quality Flowers and Vegetables Seedling, Zhengzhou, Henan　450002, China

Received Date: 2021-12-07
Rev Recd Date: 2022-03-08

Available Online: 2022-04-24

Publish Date: 2022-04-28

Abstract

Abstract

Objective NnWRKY22 in Nelumbo nucifera was cloned and its expressions in various organs under copper stress analyzed. Method RT-PCR was used to clone the gene from cDNA of lotus leaves, and bioinformatics of the sequence obtained using ProtParam. The gene expressions in roots, stems, leaves, and flowers under an imposed copper stress for varied durations were detected by qRT-PCR. Result The successfully cloned NnWRKY22 had an ORF of 573 bp and encoded 190 amino acid residues. The unstable hydrophilic protein had a sequence containing a conserved WRKY domain and a molecular weight of 21.33 kDa, an isoelectric point of pH 9.03, an instability index of 71.93, and hydrophilicity of −0.692. The phylogenetic analyses revealed a close genetic relationship of the gene with those of Aquilegia coerulea and Macleaya cordata. The qRT-PCR results showed the organ-specific expressions of the gene in the tissues which were highest in the roots and lowest in the flowers. They were significantly upregulated and peaked in 7 d by the copper treatment. Conclusion NnWRKY22 was successfully isolated from lotus and cloned. The gene was most highly expressed in the lotus roots and significantly upregulated by the presence of copper. It presumably played an important role in the response of the lotus plants to copper stress.
- Lotus,
- NnWRKY22 gene,
- expression analysis,
- copper resistance

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References(26)

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