Preparation and Efficacy of Inactivated Pseudorabies Vaccine Using Different Adjuvants

HOU Bo; WANG Chenyan; CHEN Qiuyong; WU Xuemin; LIU Yutao; WANG Longbai; ZHOU Lunjiang

doi:10.19303/j.issn.1008-0384.2022.004.001

Volume 37 Issue 4

Apr. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(4): 423-428

HOU B, WANG C Y, CHEN Q Y, et al. Preparation and Efficacy of Inactivated Pseudorabies Vaccine Using Different Adjuvants [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：423−428 doi: 10.19303/j.issn.1008-0384.2022.004.001

Citation:

HOU B, WANG C Y, CHEN Q Y, et al. Preparation and Efficacy of Inactivated Pseudorabies Vaccine Using Different Adjuvants [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：423−428 doi: 10.19303/j.issn.1008-0384.2022.004.001

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Preparation and Efficacy of Inactivated Pseudorabies Vaccine Using Different Adjuvants

doi: 10.19303/j.issn.1008-0384.2022.004.001

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou, Fujian　350013, China

Received Date: 2022-01-18
Rev Recd Date: 2022-03-05

Available Online: 2022-06-19

Publish Date: 2022-04-28

Abstract

Abstract

Objective Immune efficacy of inactivated pseudorabies vaccine prepared with different adjuvants were compared. Methods Using 3 different adjuvants as base, inactivated vaccines against the novel variants of pseudorabies virus (PRV), FJ-2012, were prepared from the gE/gI-deleted virus. After a quality assessment, these water-phase GEL02-, amphoteric ISA 206VG- or white oil adjuvant-based vaccines were injected into sheep. Detection of gB and gE antibodies in the animals were performed using ELISA. The protective efficacy against the disease in 28 d after vaccination was determined by comparing to that of an FJ-2012 challenge test. Results The successfully prepared vaccines showed no adverse reactions on the inoculated sheep. The gB antibody was tested positive, but gE negative, in the vaccinated sheep. Out of 5 sheep in the 4 test animal groups, 5 in the challenge control died in 6 d and two perished in the group received ISA 206VG-based vaccine. But all sheep in the group injected with either the experimental GEL02- or the white oil-based vaccine survived. Conclusion A perfect protection rate against FJ-2012 was demonstrated on the sheep vaccinated by the GEL02- or the white oil-based vaccine obtained from the FJ-2012ΔgE/gI strain in this study. A new venue to prevent and control the novel variants of PRV in China was made available.
- Pseudorabies virus,
- gE/gI gene deletion,
- immune efficacy,
- inactivated vaccine

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References

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