Preparation and Efficacy of Inactivated Pseudorabies Vaccine Using Different Adjuvants

  Objective  Immune efficacy of inactivated pseudorabies vaccine prepared with different adjuvants were compared.

  Methods   Using 3 different adjuvants as base, inactivated vaccines against the novel variants of pseudorabies virus (PRV), FJ-2012, were prepared from the gE/gI-deleted virus. After a quality assessment, these water-phase GEL02-, amphoteric ISA 206VG- or white oil adjuvant-based vaccines were injected into sheep. Detection of gB and gE antibodies in the animals were performed using ELISA. The protective efficacy against the disease in 28 d after vaccination was determined by comparing to that of an FJ-2012 challenge test.

  Results  The successfully prepared vaccines showed no adverse reactions on the inoculated sheep. The gB antibody was tested positive, but gE negative, in the vaccinated sheep. Out of 5 sheep in the 4 test animal groups, 5 in the challenge control died in 6 d and two perished in the group received ISA 206VG-based vaccine. But all sheep in the group injected with either the experimental GEL02- or the white oil-based vaccine survived.

  Conclusion  A perfect protection rate against FJ-2012 was demonstrated on the sheep vaccinated by the GEL02- or the white oil-based vaccine obtained from the FJ-2012ΔgE/gI strain in this study. A new venue to prevent and control the novel variants of PRV in China was made available.