Bacterial Community Structure and Diversity in Rhizosphere Soils in Healthy and Black Shank-infected Tobacco Fields

HU Qianyu; CAI Yongzhan; HAN Xiaonyu; FU Zongwei; LIU Shun; YANG Zuheng; LEI Lei; CHEN Xiaolong; FANG Yu; YU Lei; HUANG Feiyan

doi:10.19303/j.issn.1008-0384.2022.002.013

Volume 37 Issue 2

Feb. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(2): 233-239

HU Q Y, CAI Y Z, HAN X N, et al. Bacterial Community Structure and Diversity in Rhizosphere Soils in Healthy and Black Shank-infected Tobacco Fields [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：233−239 doi: 10.19303/j.issn.1008-0384.2022.002.013

Citation:

HU Q Y, CAI Y Z, HAN X N, et al. Bacterial Community Structure and Diversity in Rhizosphere Soils in Healthy and Black Shank-infected Tobacco Fields [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：233−239 doi: 10.19303/j.issn.1008-0384.2022.002.013

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Bacterial Community Structure and Diversity in Rhizosphere Soils in Healthy and Black Shank-infected Tobacco Fields

doi: 10.19303/j.issn.1008-0384.2022.002.013

1.
Kunming University/Engineering Research Center for Characteristics Agriculture of Yunnan Province, Kunming, Yunnan　650214, China
2.
Qujing City Corporation of Tobacco Companies in Yunnan Province, Qujing, Yunnan　655000, China
3.
Xuanwei Agricultural Technique Extension Center, Xuanwei, Yunnan　655400, China
4.
Tobacco Procurement Center, China Tobacco Co., Ltd., Zhengzhou, Henan　450000, China

Received Date: 2021-12-08
Rev Recd Date: 2021-12-29
Publish Date: 2022-02-25

Abstract

Abstract

Objective Bacterial community structure and diversity in the rhizosphere soils of healthy and black shank-infected tobacco fields were compared for early disease prevention and ecological regulation of the agriculture. Method Illumina MiSeq high-throughput sequencing was performed on the soil samples to determine the bacterial community composition in the rhizosphere soil. Result There were 4 763 bacterial OTUs in the rhizosphere soil at the lot of healthy tobacco plants and 4 653 in that of the diseased plants. The reductions on total number of OTUs was 2.31% and 3% on the unique OTUs as affected by the infecting disease on the plants. The microbial diversity was significantly reduced, as the Shannon, ACE, and Chao1 indices declined by 3.05%, 21.29%, and 15.23%, respectively. The dominant species in them were significantly different as well, with the relative abundance of Bacteroidota and Myxococcotain decreased by 59.13% and 20.38%, respectively, while those of Firmicutes and Methylomirabilota increased by 34.17% and 117.19%, respectively. At genus level, Nocardioides predominantly presented in the rhizosphere soil of healthy tobacco plants at 4.99%, but not detected at the lot infected by black shank. Conclusion The bacterial community structure was significantly altered and species diversity significantly decreased in the rhizosphere soil as the tobacco plants were infected with the black shank disease.
- Black shank-infected tobacco,
- bacterial community structure,
- rhizosphere soil,
- diversity

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References(23)

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