Changes in Cell Structure and Gene Expression of Lily Leaves with Necrosis

GE Jintao; WANG Jiangying; TANG Xueyan; SUN Mingwei; TENG Nianjun; ZHU Pengbo; ZHAO Tongli; WU Qiuyue; SHAO Xiaobin

doi:10.19303/j.issn.1008-0384.2022.002.012

Volume 37 Issue 2

Feb. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(2): 224-232

GE J T, WANG J Y, TANG X Y, et al. Changes in Cell Structure and Gene Expression of Lily Leaves with Necrosis [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：224−232 doi: 10.19303/j.issn.1008-0384.2022.002.012

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GE J T, WANG J Y, TANG X Y, et al. Changes in Cell Structure and Gene Expression of Lily Leaves with Necrosis [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：224−232 doi: 10.19303/j.issn.1008-0384.2022.002.012

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Changes in Cell Structure and Gene Expression of Lily Leaves with Necrosis

doi: 10.19303/j.issn.1008-0384.2022.002.012

1.
Lianyungang Academy of Agricultural Sciences, Lianyungang, Jiangsu　222000, China
2.
College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu　210095, China
3.
Bureau of Agricultural and Rural Affairs of Haizhou District, Lianyungang, Jiangsu　222006, China

Received Date: 2021-11-19
Accepted Date: 2021-11-19
Rev Recd Date: 2021-12-30
Publish Date: 2022-02-25

Abstract

Abstract

Objective Changes in the cell structure and gene expression of leaf caused by the pathogenesis of lily upper leaf necrosis (ULN) were studied to understand the molecular mechanism of the disease on Oriental Lily Tarrango (Lilium tarrango) . Methods Ultrastructure of the leaves from normal as well as mildly and severely ULN-infected lily plants was examined under a scanning electron microscope (SEM) and a projection electron microscope (TEM). Transcriptome sequences of the leaf specimens with or without calcium spraying were compared to identify the differentially expressed genes. Result The size of epidermal cells on the adaxial leaf surface shrank as the ULN worsened with vacuolar water loss. A total of 7 185 differentially expressed genes were identified that included 5 860 specific and 1 325 common differentially expressed genes. The KEGG enrichment analysis showed that the genes were enriched in the metabolic pathways, pyruvate metabolism, biosynthesis of secondary metabolites, and carbon fixation of photosynthetic organisms in all 4 groups of specimens with or without calcium spraying. FOLK, PLD1_2, ATPeF1B, KCS, CALM, ENO, and pel were significantly downregulated during the progress of ULN on the leaves. After the calcium spraying, CALM, CPK, EIN2, AUX1, PLD1_2, and SORD were upregulated. Conclusion Calcium deficiency was deemed to be the key factor that led to ULN on the lily plants. The deficiency produced the symptoms, such as vacuolar moisture loss and cellular shrinkage, might be regulated by the hormone metabolisms related to abscisic acid, ethylene, and auxin.
- Oriental Lily Tarrango,
- lily upper leaf necrosis,
- electron microscopy,
- transcriptome,
- differentially expressed gene

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References(24)

References

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