Effects of Temperature and Media on Cocultivation of <i>Pleurotus pulmonarius</i> and <i>Trichoderma harzianum</i>

LAN Qingxiu; KE Binrong; LU Zhenghui; MA Lu; HUANG Chenyang; LIAO Jianhua

doi:10.19303/j.issn.1008-0384.2022.002.009

Volume 37 Issue 2

Feb. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(2): 197-202

LAN Q X, KE B R, LU Z H, et al. Effects of Temperature and Media on Cocultivation of Pleurotus pulmonarius and Trichoderma harzianum [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：197−202 doi: 10.19303/j.issn.1008-0384.2022.002.009

Citation:

LAN Q X, KE B R, LU Z H, et al. Effects of Temperature and Media on Cocultivation of Pleurotus pulmonarius and Trichoderma harzianum [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：197−202 doi: 10.19303/j.issn.1008-0384.2022.002.009

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Effects of Temperature and Media on Cocultivation of Pleurotus pulmonarius and Trichoderma harzianum

doi: 10.19303/j.issn.1008-0384.2022.002.009

LAN Qingxiu^{1, 2, 3
,},
KE Binrong^{1, 3},
LU Zhenghui^{1, 3
,
,},
MA Lu^{1, 3},
HUANG Chenyang²,
LIAO Jianhua^{1, 3}

1.
Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing　100081, China
3.
National and Local Joint Engineering Research Center (NDRC) for Breeding & Cultivation of Featured Edible Fungi, Fuzhou, Fujian　350014, China

Received Date: 2021-09-07
Rev Recd Date: 2022-01-28
Publish Date: 2022-02-25

Abstract

Abstract

Objective Growth and characteristics of 5 strains of Pleurotus pulmonarius and Trichoderma harzianum in cocultivation on different media at different temperatures were studied for the potential application in the mushroom cultivation. Method The growth rate and plate confrontation culture methods were used to observe the mycelial growth or antagonism of 5 strain of P. pulmonarius and T. harzianum in cocultivation on different media at different temperatures. Result Under same culture conditions, the mycelial growth of the mushroom strain 86-1 significantly differed from the rest of the 5 strains. The growth of Y710-14 at 25 ℃and 28 ℃ showed no significant differences but did from other 3 tested temperatures. The mycelial growth rates of X98ly-13, XD-13, 903-1, and 86-1 at 25-30 ℃did not differ significantly but significantly at 32 ℃and 34 ℃. Both T. harzianum and P. pulmonarius grew well at 25-32 ℃. The former grew significantly faster than the latter, and thus, covered all over the latter in time of coculture on medium. However, no apparent antagonism between the two species was evident. Insofar as culture medium is concerned, PDYA provided the strongest mycelial growth followed by PDSYA, while PDSA and PDA being average. Among the various strains, 86-1 grew the slowest on any of the media. On PDA or PDSYA, X98ly-13, Y710-14, XD-13, and 903-1 grew without any significant differences, so did X98ly-13, Y710-14, or 903-1 on PDSA. When starch was present as an ingredient in PDSA, the strains of mushrooms appeared whiter with milky white color, and no significant differences on the mycelia growth rate of X98ly-13, Y710-14, and 901-1. Conclusion The optimum growth temperature for Y710-14 was 30 ℃, and for the other strain 25 ℃. Both P. pulmonarius and T. harzianum grew well in same range of temperature, but the mushroom mycelia grew slower with lower tolerance to high temperature than T. harzianum. The medium containing yeast powder produced thicker mycelia with the strongest vitality, while that consisted of starch, whiter in color than otherwise. It appeared that there was an application potential of the cocultivation of P. pulmonarius and T. harzianum for the mushroom industry.
- Pleurotus pulmonarius,
- temperature,
- culture medium,
- Trichoderma harzianum

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References(25)

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