Effect of Temperature Lowering Rate on Physiological Response of <i>Oplegnathus punctatus</i> in Live Fish Chill Preservation

HUA Maozhen; HUANG Baosheng; YU Taotao; JIANG Lexia; ZHANG Jiaguo; ZHANG Changfeng; SUN Chongde

doi:10.19303/j.issn.1008-0384.2022.002.003

Volume 37 Issue 2

Feb. 2022

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Fujian Journal of Agricultural Sciences > 2022 > 37(2): 146-155. > DOI: 10.19303/j.issn.1008-0384.2022.002.003

HUA M Z, HUANG B S, YU T T, et al. Effect of Temperature Lowering Rate on Physiological Response of Oplegnathus punctatus in Live Fish Chill Preservation [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：146−155. DOI: 10.19303/j.issn.1008-0384.2022.002.003

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Effect of Temperature Lowering Rate on Physiological Response of Oplegnathus punctatus in Live Fish Chill Preservation

HUA Maozhen^{1, 2,},
HUANG Baosheng^{2, 3, ,},
YU Taotao^{1, 2},
JIANG Lexia^{2, 4},
ZHANG Jiaguo^{2, 3, ,},
ZHANG Changfeng^{2, 3},
SUN Chongde^{2, 3}

1.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Oceanic University, Shanghai　201306, China
2.
Shandong Key Laboratory of Storage and Transportation Technology of Agricultural Products, Jinan, Shandong　250103, China
3.
National Engineering Research Center for Agricultural Products Logistics, Jinan, Shandong　 250103, China
4.
Guangdong Oceanic University, Zhanjiang, Guangdong　524000, China

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Received Date: August 25, 2021
Revised Date: November 24, 2021
Available Online: February 06, 2022

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Abstract

Abstract

Objective Physiological response of Oplegnathus punctatus to the rate of temperature lowering was studied to optimize the live fish chill preservation technology for water-conservation transportation.
Method Live and healthy O. punctatus were chilled at a rate of 1, 3, or 5 ℃·h⁻¹ from 17.5–18.5 ℃ to the critical dormancy temperature of 10 ℃. Kept the fish out of water, in intervals of 0, 2, 4, and 6 h, sampling for the determination of various physiological indicators was conducted for a data analysis.
Result The highest survival rate of O. punctatus in 6 h after the treatments was 76.7% with a chilling rate of 3 ℃·h⁻¹. Under the chilling and low-temp preservation, the contents of cortisol, AST, ALT, MDA, GLU, and LAC in the fish of all treatment groups increased significantly (P＜0.05), while those of EPI, hepatic glycogen, and muscle glycogen significantly declined (P＜0.05). The serum indices of the fish chilled at all 3 rates for 6 h at 10 ℃ changed significantly (P＜0.05). The contents of EPI, cortisol, liver ALT, and hepatic glycogen were significantly higher under the 1 ℃·h⁻¹ than the 3 ℃·h⁻¹ or 5 ℃·h⁻¹ treatment (P＜0.05) indicating a stronger stress response of the fish by the temperature lowering rate at 1 ℃·h⁻¹ than the other two; while AST, MDA, and LAC were significantly higher under the 5 ℃·h⁻¹ than the 1 ℃·h⁻¹ or 3 ℃·h⁻¹ treatment (P＜0.05) and EPI, AST, ALT, MDA, LAC, and hepatic glycogen significantly lower under the 3 ℃·h⁻¹ than the 1 ℃·h⁻¹ or 5 ℃·h⁻¹ treatment (P＜0.05) showing the 3 ℃·h⁻¹ rate of chilling minimized the effect of cold stress and lessen the liver injury on O. punctatus in the preservation process.
Conclusion It appeared that a temperature lowering rate, such as 3 ℃·h⁻¹, could be a tolerable threshold for the fish to make adequate acclimation to recover from physiological ill-effects with a high survival rate out of water at 10 ℃ for 6 h.
- Oplegnathus punctatus,
- temperature lowering rate,
- survival without water,
- stress response

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