Mapping of Early Senescence-related <i>OsBRCA</i>1 in Rice by BSA-seq Technique

JIANG Jiahuan; ZHU Yongsheng; CHEN Liping; ZHENG Yanmei; CAI Qiuhua; XIE Huaan; WANG Airong; ZHANG Jianfu

doi:10.19303/j.issn.1008-0384.2022.002.001

Volume 37 Issue 2

Feb. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(2): 131-137

JIANG J H, ZHU Y S, CHEN L P, et al. Mapping of Early Senescence-related OsBRCA1 in Rice by BSA-seq Technique [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：131−137 doi: 10.19303/j.issn.1008-0384.2022.002.001

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JIANG J H, ZHU Y S, CHEN L P, et al. Mapping of Early Senescence-related OsBRCA1 in Rice by BSA-seq Technique [J]. Fujian Journal of Agricultural Sciences，2022，37（2）：131−137 doi: 10.19303/j.issn.1008-0384.2022.002.001

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Mapping of Early Senescence-related OsBRCA1 in Rice by BSA-seq Technique

doi: 10.19303/j.issn.1008-0384.2022.002.001

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences/Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding in South China, Ministry of Agriculture, P.R. China/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian　350003, China
2.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2022-01-09
Rev Recd Date: 2022-02-10

Available Online: 2022-03-21

Publish Date: 2022-02-25

Abstract

Abstract

Objective Molecular mechanism of rice senescence was studied by locating and cloning the specific gene from a mutant artificially induced with early senescence. Methods Japonica rice Yunyin was treated with EMS to artificially induce a genetically stable early senescence w14. The mutant was crossed with Nipponbare to construct an isolated F₂ population. Subsequently, equal parts of the DNA of 100 recessive and dominant plants in the population were mixed. The differential loci between these two DNA pools were analyzed by BSA-seq to locate the target early senescence-related gene. Results The early senescence phenotype of w14 was found to be controlled by a single recessive gene. The mutagenic gene mapped and identified by the specific sequence showed the main peak located in Chr 3 and the candidate interval Chr 3: 27.5–29.5 Mb. In the region, two qualified candidate causal variants were found. Conclusion The mutation site of the candidate gene Loc_ Os03g49210 located in the 2^nd exon was determined to have changed in the wild-type from C to T. It was a non-synonymous mutation that resulted in the 20^th amino acid encoded by the gene changed from T (threonine) to I (isoleucine) and led to the functional alternation. Being homologous with human BRCA1, the target gene was named OsBRCA1.
- rice,
- mutant,
- early senescence,
- gene clone,
- BSA-seq

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References(19)

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