Inhibitory Effect of Lipopeptide Secreted by<i> Bacillus</i> Strain FJAT-2349  Against Anthracnose Pathogen

CHEN Meichun; DENG YingJie; CHEN Yanping; XIAO Rongfeng; ZHENG Xuefang; ZHU Yujing; WANG Jieping; LIU Bo

doi:10.19303/j.issn.1008-0384.2021.12.012

Volume 36 Issue 12

Dec. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(12): 1478-1483

CHEN M C, DENG Y J, CHEN Y P, et al. Inhibitory Effect of Lipopeptide Secreted by Bacillus Strain FJAT-2349 Against Anthracnose Pathogen [J]. Fujian Journal of Agricultural Sciences，2021，36（12）：1478−1483 doi: 10.19303/j.issn.1008-0384.2021.12.012

Citation:

CHEN M C, DENG Y J, CHEN Y P, et al. Inhibitory Effect of Lipopeptide Secreted by Bacillus Strain FJAT-2349 Against Anthracnose Pathogen [J]. Fujian Journal of Agricultural Sciences，2021，36（12）：1478−1483 doi: 10.19303/j.issn.1008-0384.2021.12.012

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Inhibitory Effect of Lipopeptide Secreted by Bacillus Strain FJAT-2349 Against Anthracnose Pathogen

doi: 10.19303/j.issn.1008-0384.2021.12.012

1.
Agricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei　430000, China

Received Date: 2021-09-02
Rev Recd Date: 2021-10-27
Publish Date: 2021-12-28

Abstract

Abstract

Objective Inhibitory effect of lipopeptides secreted by Bacillus FJAT-2349 on the pathogen of anthracnose was investigated. Method Agar plate diffusion and pin-prick inoculation were used to determine the inhibition effect of the lipopeptides on the pathogen. The fungal morphology was observed under a scanning and a transmission electron microscope. Result The lipopeptide-producing B. amyloliquefaciens FJAT-2349 could effectively inhibit the growth of Colletotrichum acutatum with an inhibition rate of 87.8%. The lipopeptides were composed of mainly fengycin, but also iturin and surfactin. The diameter of inhibition zone on the agar plate by the lipopeptide against C. acutatum was up to 22 mm. The effect was dose-dependent. Under the electron microscopes, the fungal hyphae appeared deformed and cell walls damaged by the lipopeptides. Inoculation of FJAT-2349 on harvested loquats could delay the onset of anthracnose by one day. The in vivo tests showed that on the 4^th day after inoculation the relative biocontrol efficacy on a curative-treatment group was 29.2% and 72.7% on a preventive-treatment group; and the average lesion diameter on the treatment groups was 4.67-5.00 mm, as opposed to the larger 6.89-7.25 mm of control. Conclusion The lipopeptides secreted by B. amyloliquefaciens FJAT-2349 could effectively inhibit the growth of Colletotrichum acutatum.
- Bacillus lipopeptide,
- loquat anthracnose,
- antifungal effect,
- disease incidence

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References(19)

References

[1]	何志刚, 李维新, 林晓姿, 等. 枇杷果实成熟和贮藏过程中有机酸的代谢 [J]. 果树学报, 2005, 22(1):23−26. HE Z G, LI W X, LIN X Z, et al. Organic acids metabolism of loquat fruit during maturity and storage [J]. Journal of Fruit Science, 2005, 22(1): 23−26.(in Chinese)
[2]	陶金华, 刘岱, 陈发河. 炭疽病侵染对枇杷果实病程相关酶活性的影响 [J]. 天津农业科学, 2011, 17(5):1−4. doi: 10.3969/j.issn.1006-6500.2011.05.001 TAO J H, LIU D, CHEN F H. Effect of Colletotrichum gloeosporioides infection on relevant enzymes changes of loquat fruit [J]. Tianjin Agricultural Sciences, 2011, 17(5): 1−4.(in Chinese) doi: 10.3969/j.issn.1006-6500.2011.05.001
[3]	王华东. 热空气和MeJA处理对枇杷采后炭疽病的防治效果及其机理研究[D]. 南京: 南京农业大学, 2014. WANG H D. Study on effects and mechanisms of hot air treatment and MeJA treatment on postharvest antharcnose rot of loquat fruit [D]. Nanjing: Nanjing Agriculture University, 2011. (in Chinese)
[4]	彦群, 杨叶, 黄圣明. 枇杷果实病害及防腐保鲜 [J]. 广西热带农业, 2001, 81(4):16−18. YAN Q, YANG Y, HUANG S M. Research on loquat fruit diseases, antiseptic and fresh-keeping [J]. Guangxi Science & Technology of Tropical Crops, 2001, 81(4): 16−18.(in Chinese)
[5]	柳志强, 郑媛, 吴亚腾, 等. 阴香内生细菌YX6的鉴定及对香蕉炭疽病的防效 [J]. 西南农业学报, 2014, 27(6):2402−2404. LIU Z Q, ZHENG Y, WU Y T, et al. Study on identification of cinnamomum burmannii endophytic bacterial strain YX6 and its control effect on Colletotrichum musae [J]. Southwest China Journal of Agricultural Sciences, 2014, 27(6): 2402−2404.(in Chinese)
[6]	黄现青, 别小妹, 吕凤霞, 等. 枯草芽孢杆菌fmbJ产脂肽抑制点青霉效果及其桃防腐试验 [J]. 农业工程学报, 2008(1):263−267. doi: 10.3321/j.issn:1002-6819.2008.01.052 HUANG X Q, BIE X M, LYU F X, et al. Inhibitory effect of lipopeptides from Bacillus Subtilis fmbJ on Penicillium notatum and its antisepsis to peach [J]. Transactions of the Chinese Society of Agricultural Engineering, 2008(1): 263−267.(in Chinese) doi: 10.3321/j.issn:1002-6819.2008.01.052
[7]	TORAL L, RODRÍGUEZ M, BÉJAR V, et al. Antifungal activity of lipopeptides From Bacillus XT1 CECT 8661 against Botrytis cinerea [J]. Front Microbiol, 2018, 9: 1315. doi: 10.3389/fmicb.2018.01315
[8]	CALVO H, MENDIARA I, ARIAS E, et al. The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots [J]. Food Microbiol, 2019, 82: 62−69. doi: 10.1016/j.fm.2019.01.010
[9]	JIN P F, WANG H N, TAN Z, et al. Antifungal mechanism of bacillomycin D from Bacillus velezensis HN-2 against Colletotrichum gloeosporioides Penz [J]. Pesticide Biochemistry and Physiology, 2020, 163: 102−107. doi: 10.1016/j.pestbp.2019.11.004
[10]	LIN F X, HUANG Z H, CHEN Y R, et al. Effect of combined bacillomycin D and chitosan on growth of Rhizopus stolonifer and Botrytis cinerea and cherry tomato preservation [J]. J Sci Food Agric, 2021, 101(1): 229−239. doi: 10.1002/jsfa.10635
[11]	CHEN M C, WANG J P, ZHU Y J, et al. Antibacterial activity against Ralstonia solanacearum of the lipopeptides secreted from the Bacillus amyloliquefaciens strain FJAT-2349 [J]. J Appl Microbiol, 2019, 126(5): 1519−1529. doi: 10.1111/jam.14213
[12]	PRAMUDITO T E, AGUSTINA D, NGUYEN T K N, et al. A novel variant of narrow-spectrum antifungal bacterial lipopeptides that strongly inhibit Ganoderma boninense [J]. Probiotics Antimicrob Proteins, 2018, 10(1): 110−117. doi: 10.1007/s12602-017-9334-2
[13]	AIT KAKI A, SMARGIASSO N, ONGENA M, et al. Characterization of new fengycin cyclic lipopeptide variants produced by Bacillus amyloliquefaciens (ET) originating from a salt lake of eastern algeria [J]. Curr Microbiol, 2020, 77(3): 443−451. doi: 10.1007/s00284-019-01855-w
[14]	GONG A D, LI H P, YUAN Q S, et al. Antagonistic mechanism of iturin A and plipastatin A from Bacillus amyloliquefaciens S76-3 from wheat spikes against Fusarium graminearum [J]. Plos one, 2015, 10: 1−18.
[15]	ZHAI L, REN R, MENG D, et al. Comparison of aminotransferases of three Bacillus strains Bacillus altitudinis W3, Bacillus velezensis SYBC H47, and Bacillus amyloliquefaciens YP6 via genome analysis and bioinformatics [J]. J Appl Genet, 2019, 60(3-4): 427−430. doi: 10.1007/s13353-019-00512-z
[16]	MIHALACHE G, BALAES T, GOSTIN I, et al. Lipopeptides produced by Bacillus subtilis as new biocontrol products against fusariosis in ornamental plants [J]. Environ Sci Pollut Res Int, 2018, 25(30): 29784−29793. doi: 10.1007/s11356-017-9162-7
[17]	CHO S J, LEE S K, CHA B J, et al. Detection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03 [J]. FEMS Microbiology Letters, 2003, 223: 47−51. doi: 10.1016/S0378-1097(03)00329-X
[18]	HIRADATE S, YOSHIDA S, SUGIE H, et al. Mulberry anthracnose antagonists (iturins) produced by Bacillus amyloliquefaciens RC-2 [J]. Phytochemistry, 2002, 61: 693−698. doi: 10.1016/S0031-9422(02)00365-5
[19]	KIM P I, BAI H, BAI D, et al. Purification and characterization of a lipopeptide produced by Bacillus thuringiensis CMB26 [J]. Journal of Applied Microbiology, 2004, 97: 942−949. doi: 10.1111/j.1365-2672.2004.02356.x