Identification and Biological Characteristics of Stem Rot Pathogen of <i>Dendrobium officinale</i>

YAO Jinai; HUANG Peng; LAN Chengzhong; HOU Xiangyu; YU Deyi

doi:10.19303/j.issn.1008-0384.2021.12.009

Volume 36 Issue 12

Dec. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(12): 1457-1463

YAO J A, HUANG P, LAN C Z, et al. Identification and Biological Characteristics of Stem Rot Pathogen of Dendrobium officinale [J]. Fujian Journal of Agricultural Sciences，2021，36（12）：1457−1463 doi: 10.19303/j.issn.1008-0384.2021.12.009

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YAO J A, HUANG P, LAN C Z, et al. Identification and Biological Characteristics of Stem Rot Pathogen of Dendrobium officinale [J]. Fujian Journal of Agricultural Sciences，2021，36（12）：1457−1463 doi: 10.19303/j.issn.1008-0384.2021.12.009

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Identification and Biological Characteristics of Stem Rot Pathogen of Dendrobium officinale

doi: 10.19303/j.issn.1008-0384.2021.12.009

Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences/Fujian Engineering Research Center for Green Pest Management, Fuzhou, Fujian　350013, China

Received Date: 2021-10-20
Accepted Date: 2021-10-20
Rev Recd Date: 2021-11-19

Available Online: 2021-12-30

Publish Date: 2021-12-28

Abstract

Abstract

Objective To isolate and identify and determine the biological characteristics of the pathogen that caused the stem rot disease on Dendrobium officinale. Method Typical diseased plants were collected from 3 farms in Zhaoan and Songxi for pathogen isolation. Morphological and molecular identification methods including gene sequencing of ITS, EF1-α, ACT, RPB2, and LSU were performed on the isolates. Biological characteristics of the pathogen in response to temperature, pH, light condition, carbon source, and nitrogen source were determined. Result The pathogen was identified as Fusarium oxysporum, which grew optimally at 30 ℃ and pH 7 under light on a medium using starch for carbon and peptone for nitrogen sources. Conclusion F. oxysporum was identified to be the pathogen that caused the stem rot disease on D. officinale in Fujian, and the optimal mycelial growth conditions determined.
- Dendrobium officinale,
- stem rot,
- identification,
- Fusarium oxysporum,
- biological characteristics

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References(24)

References

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