EST-SNP Marker-based Genetic Analysis on Tea Germplasms of Yunxiao in Fujian

WANG Zehan; YU Wentao; FANG Deyin; CAI Jieying; WANG Jinhuan; FAN Xiaojing; LIU Caiguo; XU Biao; YE Naixing

doi:10.19303/j.issn.1008-0384.2021.12.006

Volume 36 Issue 12

Dec. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(12): 1431-1438

WANG Z H, YU W T, FANG D Y, et al. EST-SNP Marker-based Genetic Analysis on Tea Germplasms of Yunxiao in Fujian [J]. Fujian Journal of Agricultural Sciences，2021，36（12）：1431−1438 doi: 10.19303/j.issn.1008-0384.2021.12.006

Citation:

WANG Z H, YU W T, FANG D Y, et al. EST-SNP Marker-based Genetic Analysis on Tea Germplasms of Yunxiao in Fujian [J]. Fujian Journal of Agricultural Sciences，2021，36（12）：1431−1438 doi: 10.19303/j.issn.1008-0384.2021.12.006

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EST-SNP Marker-based Genetic Analysis on Tea Germplasms of Yunxiao in Fujian

doi: 10.19303/j.issn.1008-0384.2021.12.006

1.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Technology Centre of Fuzhou Customs District P.R. China, Fuzhou, Fujian　350001, China
3.
Yunxiao Institute of Tea Science, Yunxiao, Fujian　363300, China
4.
Society of Subtropical Crops in Fujian Province, Fuzhou, Fujian　350001, China

Received Date: 2021-08-14
Rev Recd Date: 2021-10-24
Publish Date: 2021-12-28

Abstract

Abstract

Objective Genetic diversity and structure of the tea germplasms in Yunxiao were studied to facilitate the collection and utilization of the natural resource. Method SNP molecular marker was used to analyze the genetic relationship and diversity of 61 varieties of Camellia sinensis in Yunxiao, Fujian. Result The mean polymorphism information index, observed and expected heterozygosity, fixed index, and minor allele frequency of the SNP marker were determined to be 0.415, 0.309, 0.260, −0.072, and 0.197, respectively. Sixty-six high quality loci that could effectively and accurately identify the genetic relationship of tea plants were obtained. The genetic distance of 6 populations ranged from 0.150 to 0.926. The DNA fingerprints of the germplasms were constructed. The principal coordinates analyses and cluster analyses showed the component distribution to be relatively close within a population and the exchanges among the groups evident. Of the varieties, Xiaomaoshan was the richest in genetic diversity. Conclusion The genetic diversity of tea germplasms in Yunxiao was vast with populations of high geographic differentiation and in-between group exchanges. The SNP molecular marker-based technology was proven applicable for identifying the germplasms benefitting the utilization of local tea resource for selection and breeding.
- Yunxiao,
- tea plant,
- germplasm resources,
- genetic diversity,
- SNP

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References(24)

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