Embryo Rescue of Interspecific <i>Brassica campestris</i> x <i>B. albograbra </i>Hybrids

LYU Fengxian; LI Chongjuan; YANG Ding; HU Jingfeng; YANG Hongli; LAN Mei; XU Xuezhong; ZHANG Liqin; HE Jiangming; DONG Xiangshu

doi:10.19303/j.issn.1008-0384.2021.11.005

Volume 36 Issue 11

Nov. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(11): 1288-1294

LYU F X, LI C J, YANG D, et al. Embryo Rescue of Interspecific Brassica campestris x B. albograbra Hybrids [J]. Fujian Journal of Agricultural Sciences，2021，36（11）：1288−1294 doi: 10.19303/j.issn.1008-0384.2021.11.005

Citation:

LYU F X, LI C J, YANG D, et al. Embryo Rescue of Interspecific Brassica campestris x B. albograbra Hybrids [J]. Fujian Journal of Agricultural Sciences，2021，36（11）：1288−1294 doi: 10.19303/j.issn.1008-0384.2021.11.005

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Embryo Rescue of Interspecific Brassica campestris x B. albograbra Hybrids

doi: 10.19303/j.issn.1008-0384.2021.11.005

LYU Fengxian^{1, 2
,},
LI Chongjuan^{1, 2},
YANG Ding^{1, 2},
HU Jingfeng^{2, 3},
YANG Hongli^{2, 3},
LAN Mei^{2, 3},
XU Xuezhong^{2, 3},
ZHANG Liqin^{2, 3},
HE Jiangming^{2, 3
,
,},
DONG Xiangshu¹

1.
Yunnan University, Kunming, Yunnan　650504, China
2.
Horticultural Crops Research Institute, Yunnan Academy Agricultural Science, Kunming, Yunnan　650205, China
3.
Yunnan Branch of National Vegetable Improvement Center, Kunming, Yunnan　650205, China

Received Date: 2021-05-31
Rev Recd Date: 2021-10-14

Available Online: 2021-12-30

Publish Date: 2021-11-28

Abstract

Abstract

Objective Methods suitable for embryo rescue in the interspecific hybridization between Brassica campestris and B. albograbra were investigated. Method The interspecific crossing was carried out using B. campestris L. as female parent and B. albograbra L. H. Bailey as male parent. An embryo rescue culture for the hybridization was conducted with 8 different media at 6 sampling times followed subsequently by 4 proliferation media and 9 rooting media. Result The embryo genesis rate at 26.32% of ERM₄ medium consisting of MS, 0.5mg 6-BA·L⁻¹, 0.5mg NAA·L⁻¹, 1.0mg KT·L⁻¹, and 1.0g AC·L⁻¹ was the highest among all. The samples collected 12d after pollination had the best rate of 26.32%, DM₁ (i.e., MS+ 0.9mg 6-BA·L⁻¹+0.1mg NAA·L⁻¹) the greatest proliferation coefficient of 5.37, and RM_4-2 (i.e., 1/2MS+ 0.5mg IBA·L⁻¹) the top rate of 37.21% and average 8.33d on rooting. Conclusion The optimum conditions for the embryo rescue in the interspecific hybridization between B. campestris L. and B. albograbra L. H. Bailey included sampling at 12d after pollination and following by culture on ERM₄ medium for embryo induction, on DM₁ for ovule proliferation, and on RM_4-2 for seedling rooting.
- Brassica campestris L.,
- Brassica albograbra L. H. Bailey,
- interspecies cross,
- embryo rescue,
- proliferation,
- adventitious root induction

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References(23)

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