Embryo Rescue of Interspecific Brassica campestris x B. albograbra Hybrids

  Objective  Methods suitable for embryo rescue in the interspecific hybridization between Brassica campestris and B. albograbra were investigated.

  Method  The interspecific crossing was carried out using B. campestris L. as female parent and B. albograbra L. H. Bailey as male parent. An embryo rescue culture for the hybridization was conducted with 8 different media at 6 sampling times followed subsequently by 4 proliferation media and 9 rooting media.

  Result  The embryo genesis rate at 26.32% of ERM₄ medium consisting of MS, 0.5mg 6-BA·L⁻¹, 0.5mg NAA·L⁻¹, 1.0mg KT·L⁻¹, and 1.0g AC·L⁻¹ was the highest among all. The samples collected 12d after pollination had the best rate of 26.32%, DM₁ (i.e., MS+ 0.9mg 6-BA·L⁻¹+0.1mg NAA·L⁻¹) the greatest proliferation coefficient of 5.37, and RM_4-2 (i.e., 1/2MS+ 0.5mg IBA·L⁻¹) the top rate of 37.21% and average 8.33d on rooting.

  Conclusion  The optimum conditions for the embryo rescue in the interspecific hybridization between B. campestris L. and B. albograbra L. H. Bailey included sampling at 12d after pollination and following by culture on ERM₄ medium for embryo induction, on DM₁ for ovule proliferation, and on RM_4-2 for seedling rooting.