Determination of Lincosamide Antibiotics Residues in Crab Tissues

QIU Ximu; ZHU Xiaohua; BIAN Wenji; WANG Shufang; MENG Yong

doi:10.19303/j.issn.1008-0384.2021.10.016

Volume 36 Issue 10

Oct. 2021

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QIU X M, ZHU X H, BIAN W J, et al. Determination of Lincosamide Antibiotics Residues in Crab Tissues [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1231−1237 doi: 10.19303/j.issn.1008-0384.2021.10.016

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QIU X M, ZHU X H, BIAN W J, et al. Determination of Lincosamide Antibiotics Residues in Crab Tissues [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1231−1237 doi: 10.19303/j.issn.1008-0384.2021.10.016

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Determination of Lincosamide Antibiotics Residues in Crab Tissues

doi: 10.19303/j.issn.1008-0384.2021.10.016

QIU Ximu^1
,,
ZHU Xiaohua^{2, 3},
BIAN Wenji²,
WANG Shufang¹,
MENG Yong^{2, 3
,
,}

1.
College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang, Jiangsu　222005, China
2.
Jiangsu Freshwater Fisheries Research Institute, Nanjing, Jiangsu　210017, China
3.
Jiangsu Aquatic Products Quality Testing Center/Fishery Products Quality Supervision, Inspection and Testing Center of Ministry of Agriculture and Rural Affairs (Nanjing), Nanjing, Jiangsu　210017, China

Received Date: 2021-04-16
Rev Recd Date: 2021-08-31

Available Online: 2021-10-23

Publish Date: 2021-10-28

Abstract

Abstract

Objective An HPLC-MS/MS method following an optimized solid phase extraction (SPE) procedure was established for the determination of lincosamide antibiotics in crab tissues. Method SPE column was used to separate and purify the substances from crab roe samples. Lincomycin, clindamycin, and pyrimycin were the target antibiotics to be detected by the methodology. Result Among the tested materials for the chemical separation, OASIS^® HLB SPE column exhibited the highest recovery rate on the antibiotics. For the HPLC-MS/MS determination on the antibiotics, the gradient elution on an Infinity Lab poroshell 120 SB-C₁₈ column (100 mm×2.7 mm, 2.7 μm) using 0.1% formic acid for the mobile phase A and methanol for the mobile phase B under the multi-reaction monitoring mode was applied. A measurement linearity within 50 μg·L⁻¹ with a R²＞0.99 was achieved. The assay performed with a recovery rate ranging 80.5%–99%, the relative standard deviation 2.5%–5.2%, the detection limit 0.22–0.35 μg·kg⁻¹, and the quantitative limit 0.72–1.16 μg·kg⁻¹. Conclusion The newly established methodology was stable and reliable in detecting the antibiotics in crab at a reduced cost.
- river crab,
- antibiotic residues,
- solid phase extraction,
- HPLC-MS/MS,
- lincosamide

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References(29)

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