Composition and Antibacterial Activity of <i>Blumea balsamifera</i> Extracts

HU Xuan; WANG Kai; YU Fulai; WANG Dan; XIE Xiaoli; PANG Yuxin; CHEN Hongfa

doi:10.19303/j.issn.1008-0384.2021.10.003

Volume 36 Issue 10

Oct. 2021

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HU X, WANG K, YU F L, et al. Composition and Antibacterial Activity of Blumea balsamifera Extracts [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1131−1138 doi: 10.19303/j.issn.1008-0384.2021.10.003

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HU X, WANG K, YU F L, et al. Composition and Antibacterial Activity of Blumea balsamifera Extracts [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1131−1138 doi: 10.19303/j.issn.1008-0384.2021.10.003

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Composition and Antibacterial Activity of Blumea balsamifera Extracts

doi: 10.19303/j.issn.1008-0384.2021.10.003

HU Xuan^1
,,
WANG Kai¹,
YU Fulai^{1
,
,},
WANG Dan¹,
XIE Xiaoli¹,
PANG Yuxin^{2
,
,},
CHEN Hongfa³

1.
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Engineering Research Center for Blumea balsamifera, Haikou, Hainan　571101, China
2.
School of Chinese Materia Medica Resources, Guangdong Pharmaceutical University, Yunfu, Guangdong　527500, China
3.
Hainan Ainaxiang Biotechnology Development Co., Ltd., Haikou, Hainan　570208, China

Received Date: 2021-06-03
Rev Recd Date: 2021-07-27

Available Online: 2021-10-23

Publish Date: 2021-10-28

Abstract

Abstract

Objective Chemical compositions andin vitro antibacterial activity of a variety of extracts from Blumea balsamifera (L.) DC were studied. Method Gas chromatography-mass spectrometry (GC-MS) was used to analyze the chemical compositions of B. blsamifera extracts in the forms of oil, powder, and camphor. Sensitivity, minimal inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) against the common oral anaerobes, i.e., Streptococcus mutans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Prevotella intermedia, of the products were determined by means of the cup plate, micro-broth dilution, and agar culture medium plate methods. Result There were 56 compounds identified from the oil, 19 from the power, and 8 from the camphor product. Chemically, the oil was significantly more complex than the other two, and only l-borneol was commonly found among them. The relative content of l-borneol in the oil was 13.36%, 81.40% in the powder, and 76.07% in the camphor. All 3 products exhibited varying degrees of moderate sensitivity to S. mutans, F. nucleatum, and P. intermedia, but not at all to P. gingivalis, on the in vitro antibacterial tests. They also showed varying inhibitory effects on the oral anaerobic bacteria with MICs ranging from 8.34 to 100 mg·mL⁻¹. Among them, the oil had the highest MICs ranging 8.34–16.68 mg·mL⁻¹ and MBCs 33.35–133.4 mg·mL⁻¹, but no significant difference between the powder and the camphor. In addition, since the MBCs of the powder and the camphor to P. gingivalis, the powder to S. mutans, and the camphor to P. intermedia were all greater than 200 mg·mL⁻¹, the bactericidal efficacies of the powder and the camphor were not considered highly desirable in comparison to the oil. Conclusion The various forms of B. blsamifera extracts displayed in vitro antibacterial activities against some common oral anaerobic bacteria, especially on F. nucleatum by the oil.
- Blumea balsamifera (L.) DC extracts,
- GC-MS,
- antibacterial activity,
- in vitro antibacterial activity,
- MIC

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References

[1]	张凯. 中药对口腔常见致病菌抑菌作用的初步研究 [J]. 全科口腔医学电子杂志, 2016, 3(11):18−19, 21. ZHANG K. Preliminary study on antibacterial effect of traditional Chinese medicine on common pathogenic bacteria in oral cavity [J]. General Journal of Stomatology, 2016, 3(11): 18−19, 21.(in Chinese)
[2]	周小洁, 时清, 杨圣辉. 20种中药提取物对牙龈卟啉单胞菌抑制作用的体外研究 [J]. 中国微生态学杂志, 2016, 28(12):1401−1403. ZHOU X J, SHI Q, YANG S H. Inhibitory effects of 20 kinds of Chinese medicine extracts on Porphyromonas gingivalis in vitro [J]. Chinese Journal of Microecology, 2016, 28(12): 1401−1403.(in Chinese)
[3]	PANG Y X, WANG D, FAN Z W, et al. Blumea balsamifera: A phytochemical and pharmacological review [J]. Molecules (Basel, Switzerland), 2014, 19(7): 9453−9477. doi: 10.3390/molecules19079453
[4]	胡璇, 陈振夏, 王凯, 等. 艾纳香油质量标准初步研究 [J]. 热带农业科学, 2016, 36(9):87−91. HU X, CHEN Z X, WANG K, et al. Development of quality standard for Blumea balsamifera oil [J]. Chinese Journal of Tropical Agriculture, 2016, 36(9): 87−91.(in Chinese)
[5]	国家药典委员会. 中华人民共和国药典(一部)[M]. 北京: 中国医药科技出版社, 2020: 90−91.
[6]	PANG Y X, ZHANG Y, HUANG L Q, et al. Effects and mechanisms of total flavonoids from Blumea balsamifera (L.) DC. on skin wound in rats [J]. International Journal of Molecular Sciences, 2017, 18(12): 2766. doi: 10.3390/ijms18122766
[7]	谢雪艳, 李天珍, 王万林, 等. 不同产源艾纳香油化学成分及其抗炎活性研究 [J]. 中药新药与临床药理, 2019, 30(9):1069−1076. XIE X Y, LI T Z, WANG W L, et al. Analysis of chemical constituents and anti-inflammatory activity of Blumea balsamifera oil from different sources [J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2019, 30(9): 1069−1076.(in Chinese)
[8]	李安, 刘印, 张泽望, 等. 微波辅助提取艾纳香精油的工艺优化及抗菌活性的研究 [J]. 福建农业学报, 2017, 32(7):751−755. LI A, LIU Y, ZHANG Z W, et al. The study on the microwave-assisted extraction on essential oil of Blumea balsamifera(L.)DC and antibacterial activity [J]. Fujian Journal of Agricultural Sciences, 2017, 32(7): 751−755.(in Chinese)
[9]	SAKEE U, MANEERAT S, CUSHNIE T P T, et al. Antimicrobial activity of Blumea balsamifera (Lin.) DC. extracts and essential oil [J]. Natural Product Research, 2011, 25(19): 1849−1856. doi: 10.1080/14786419.2010.485573
[10]	闻庆, 庞玉新, 胡璇, 等. 艾纳香残渣不同提取部位体外抑菌活性研究 [J]. 广东药学院学报, 2015, 31(6):713−716. doi: 10.3969/j.issn.1006-8783.2015.06.005 WEN Q, PANG Y X, HU X, et al. Study on antibacterial activity of extracts from residues of Blumea balsamifera(L.)DC. in vitro [J]. Journal of Guangdong Pharmaceutical University, 2015, 31(6): 713−716.(in Chinese) doi: 10.3969/j.issn.1006-8783.2015.06.005
[11]	王远辉, 田洪芸, 何思佳, 等. 不同方法提取艾纳香叶挥发性成分的气相色谱-质谱分析 [J]. 食品工业科技, 2012, 33(12):97−101, 105. WANG Y H, TIAN H Y, HE S J, et al. Analysis of volatile components from Blumea balsamifera(L.) DC. leaf with different extraction methods by gas chromatography-mass spectrometry [J]. Science and Technology of Food Industry, 2012, 33(12): 97−101, 105.(in Chinese)
[12]	刘兰, 黄家宇, 陈俊, 等. 苗药赤胫散提取物的体外抑菌活性研究 [J]. 中国药房, 2018, 29(10):1343−1347. doi: 10.6039/j.issn.1001-0408.2018.10.12 LIU L, HUANG J Y, CHEN J, et al. Study on in vitro bacteriostatic activity of the extracts from Miao medicine Polygonum runcinatum [J]. China Pharmacy, 2018, 29(10): 1343−1347.(in Chinese) doi: 10.6039/j.issn.1001-0408.2018.10.12
[13]	华亚南, 陈辉, 张其中. 100种中草药水提物体外抑杀无乳链球菌的药效研究 [J]. 生态科学, 2015, 34(3):26−30. HUA Y N, CHEN H, ZHANG Q Z. In vitro efficacy of aqueous extracts of 100 Chinese herbal medicines against Streptococcus agalactiate from tilapia [J]. Ecological Science, 2015, 34(3): 26−30.(in Chinese)
[14]	徐叔云, 卞如濂, 陈修. 药理实验方法学[M]. 3版. 北京: 人民卫生出版社, 2002: 1647−1662.
[15]	田程飘, 宋雅玲, 许海棠, 等. 超临界和水蒸气蒸馏提取沉香精油成分分析及抗氧化、抑菌活性对比研究 [J]. 中国中药杂志, 2019, 44(18):4000−4008. TIAN C P, SONG Y L, XU H T, et al. Composition analysis, antioxidative and antibacterial activities comparison of agarwood oils extracted by supercritical and steam distillation [J]. China Journal of Chinese Materia Medica, 2019, 44(18): 4000−4008.(in Chinese)
[16]	崔霞, 李秋艳, 李茹芳, 等. 茶多酚和茶黄素对致龋菌生长作用的研究 [J]. 医学研究生学报, 2020, 33(12):1246−1249. CUI X, LI Q Y, LI R F, et al. Study on the effect of tea polyphenols and the aflavins on the growth of cariogenic bacteria [J]. Journal of Medical Postgraduates, 2020, 33(12): 1246−1249.(in Chinese)
[17]	滕蕊, 郭宁, 赵晓丹, 等. 黄芩提取物局部治疗实验性牙周炎的研究 [J]. 中国美容医学, 2020, 29(10):115−119. TENG R, GUO N, ZHAO X D, et al. The study on local treatment of experimental periodontitis using Scutellaria baicalensis extracts [J]. Chinese Journal of Aesthetic Medicine, 2020, 29(10): 115−119.(in Chinese)
[18]	刘玉梅, 徐静舒. 五倍子抗口腔致病菌活性物质筛选及其体外抑菌效果评价 [J]. 实用口腔医学杂志, 2017, 33(4):437−441. doi: 10.3969/j.issn.1001-3733.2017.04.002 LIU Y M, XU J S. Screening and evaluation of the active substance of gallnut against oral cavity pathogens [J]. Journal of Practical Stomatology, 2017, 33(4): 437−441.(in Chinese) doi: 10.3969/j.issn.1001-3733.2017.04.002
[19]	殷其蕾, 刘勇, 詹先王, 等. 厚朴提取物对于4种常见口腔致病菌生长和黏附的作用 [J]. 中国药学杂志, 2011, 46(17):1356−1361. YIN Q L, LIU Y, ZHAN X W, et al. Evaluation of the antimicrobial properties of magnoliae officinalis cortex extract and its main components against four oral pathogenic bacteria [J]. Chinese Pharmaceutical Journal, 2011, 46(17): 1356−1361.(in Chinese)
[20]	陈振夏, 谢小丽, 庞玉新, 等. 艾纳香的生物活性及其在日化品中的应用 [J]. 香料香精化妆品, 2016(5):54−58. doi: 10.3969/j.issn.1000-4475.2016.05.014 CHEN Z X, XIE X L, PANG Y X, et al. Biological activities of Blumea balsamifera and their applications in daily chemicals [J]. Flavour Fragrance Cosmetics, 2016(5): 54−58.(in Chinese) doi: 10.3969/j.issn.1000-4475.2016.05.014
[21]	王秋萍, 何珺, 王黔阳, 等. 艾纳香中艾片纯化工艺研究 [J]. 云南农业大学学报(自然科学), 2017, 32(5):889−894. WANG Q P, HE J, WANG Q Y, et al. Study on the purification technology of L-borneolum in Blumea balsamifera [J]. Journal of Yunnan Agricultural University(Natural Science), 2017, 32(5): 889−894.(in Chinese)
[22]	YU B, RUAN M, DONG X P, et al. The mechanism of the opening of the blood-brain barrier by borneol: A pharmacodynamics and pharmacokinetics combination study [J]. Journal of Ethnopharmacology, 2013, 150(3): 1096−1108. doi: 10.1016/j.jep.2013.10.028
[23]	LEE S Y, KIM S H, HONG C Y, et al. Effects of (–)-borneol on the growth and morphology of Aspergillus fumigatus and Epidermophyton floccosom [J]. Flavour and Fragrance Journal, 2013, 28(2): 129−134. doi: 10.1002/ffj.3138
[24]	何祥久, 邱峰, 姚新生. 中药复方研究现状和思路 [J]. 化学进展, 2001, 13(6):481−485. doi: 10.3321/j.issn:1005-281X.2001.06.010 HE X J, QIU F, YAO X S. Present situation and thinking of Chinese traditional compound medicine research [J]. Progress in Chemistry, 2001, 13(6): 481−485.(in Chinese) doi: 10.3321/j.issn:1005-281X.2001.06.010
[25]	李倩, 潘亚萍. 具核梭杆菌与全身疾病相关性的研究进展 [J]. 中国微生态学杂志, 2016, 28(11):1353−1356. LI Q, PAN Y P. The relationship between Fusobacterium nucleatum and systemic diseases: Research progress [J]. Chinese Journal of Microecology, 2016, 28(11): 1353−1356.(in Chinese)