A RT-PCR Assay for Quantitative Detection of Bovine Viral Diarrhea Virus

ZHANG Kang; GUO Zhiting; QIU Zhengying; ZHANG Jingyan; WANG Lei; ZHANG Kai; Wang Guibo; LIANG Fenfen; MA Qian; LI Jianxi

doi:10.19303/j.issn.1008-0384.2021.09.007

Volume 36 Issue 9

Sep. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(9): 1042-1047

ZHANG K, GUO Z T, QIU Z Y, et al. A RT-PCR Assay for Quantitative Detection of Bovine Viral Diarrhea Virus [J]. Fujian Journal of Agricultural Sciences，2021，36（9）：1042−1047 doi: 10.19303/j.issn.1008-0384.2021.09.007

Citation:

ZHANG K, GUO Z T, QIU Z Y, et al. A RT-PCR Assay for Quantitative Detection of Bovine Viral Diarrhea Virus [J]. Fujian Journal of Agricultural Sciences，2021，36（9）：1042−1047 doi: 10.19303/j.issn.1008-0384.2021.09.007

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A RT-PCR Assay for Quantitative Detection of Bovine Viral Diarrhea Virus

doi: 10.19303/j.issn.1008-0384.2021.09.007

1.
Key laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Drug Innovation and Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS of Ministry of Agriculture, Lanzhou, Gansu　730050, China
2.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu　730070, China

Received Date: 2020-11-02
Rev Recd Date: 2021-08-30

Available Online: 2021-10-23

Publish Date: 2021-09-28

Abstract

Abstract

Objective A rapid detection and quantification method for bovine viral diarrhea virus (BVDV) was established. Method For the methodology development, specific primers and probes were designed based on the target regions of the BVDV 5′UTR gene published by GenBank. The RNA of in vitro transcription viruses was used as the absolute quantitative standard. Reaction conditions of the fluorescent quantitative RT-PCR were optimized. Result The newly developed assay had a minimum detection limit of 5.0267copies/μL and an intragroup variation coefficient of less than 1% with high repeatability and specificity. Other than BVDV, it amplified no viral nucleic acids of viruses such as swine fever and foot-and-mouth diseases. Conclusion The established fluorescence quantitative RT-PCR method was highly sensitive, specific, and repeatable in detecting and quantifying BVDV. It appeared appropriate for early diagnosis of bovine viral diarrhea.
- Bovine viral diarrhea virus,
- 5′UTR,
- RT-PCR,
- standard RNA

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References(20)

References

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