A Rapid Detection Method on RPA of Sweet Potato Leaf Curl Virus

XU Yongqing; LI Huawei; ZHANG Hong; LI Guoliang; LIN Zhaomiao; QIU Yongxiang; QIU Sixin

doi:10.19303/j.issn.1008-0384.2021.08.008

Volume 36 Issue 8

Aug. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(8): 923-926

XU Y Q, LI H W, ZHANG H, et al. A Rapid Detection Method on RPA of Sweet Potato Leaf Curl Virus [J]. Fujian Journal of Agricultural Sciences，2021，36（8）：923−926 doi: 10.19303/j.issn.1008-0384.2021.08.008

Citation:

XU Y Q, LI H W, ZHANG H, et al. A Rapid Detection Method on RPA of Sweet Potato Leaf Curl Virus [J]. Fujian Journal of Agricultural Sciences，2021，36（8）：923−926 doi: 10.19303/j.issn.1008-0384.2021.08.008

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A Rapid Detection Method on RPA of Sweet Potato Leaf Curl Virus

doi: 10.19303/j.issn.1008-0384.2021.08.008

Crop Research Institute , Fujian Academy of Agricultural Sciences/Scientific Observation and Experimentation Station on Tuber and Root Crops in South China, Ministry of Agriculture/Dry Crop Engineering Research Center of Fujian Province, Fuzhou, Fujian　350013, China

Received Date: 2021-05-24
Rev Recd Date: 2021-06-30

Available Online: 2021-08-10

Publish Date: 2021-08-28

Abstract

Abstract

Objective A rapid isothermal amplification method for detecting the recombinant enzyme polymerase (RPA) in sweet potato leaf curl virus (SPLCV) was established. Method A set of primers for RPA detection was designed based on the AV1 gene selected from SPLCV sequence. Reaction conditions for the detection was optimized and verified for applicability. Result The newly established methodology took merely 20m to complete the nucleic acid amplification at 39℃, which greatly improved the detection efficiency．More importantly, the method was specific toward SPLCV and free of any cross reaction with DNAs of other pathogens, such as TYLCD, SPFMV, SPVG, or SPCSV. It also showed a high repeatability and a minimum detection limit on RPA of 10 pg·μL⁻¹. Conclusion The newly developed method for SPLCV identification based on the detection of RPA in sweet potatoes was rapid, accurate, specific, repeatable, and easy to operate in the field.
- Sweet potato,
- sweet potato leaf curl virus（SPLCV）,
- RPA,
- detection methodology

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References(16)

References

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