Correlation Between Flavonoids Content and SSR Markers of Tartary Buckwheat

SHI Taoxiong; LI Ruiyuan; PAN Fan; HUANG Juan; ZHU Liwei; WANG Yan; LIANG Chenggang

doi:10.19303/j.issn.1008-0384.2021.08.003

Volume 36 Issue 8

Aug. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(8): 884-891

SHI T X, LI R Y, PAN F, et al. Correlation Between Flavonoids Content and SSR Markers of Tartary Buckwheat [J]. Fujian Journal of Agricultural Sciences，2021，36（8）：884−891 doi: 10.19303/j.issn.1008-0384.2021.08.003

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SHI T X, LI R Y, PAN F, et al. Correlation Between Flavonoids Content and SSR Markers of Tartary Buckwheat [J]. Fujian Journal of Agricultural Sciences，2021，36（8）：884−891 doi: 10.19303/j.issn.1008-0384.2021.08.003

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Correlation Between Flavonoids Content and SSR Markers of Tartary Buckwheat

doi: 10.19303/j.issn.1008-0384.2021.08.003

Buckwheat Industry Technology Research Center, Guizhou Normal University, Guiyang, Guizhou　550001, China

Received Date: 2021-04-25
Rev Recd Date: 2021-06-29

Available Online: 2021-08-10

Publish Date: 2021-08-28

Abstract

Abstract

Objective Correlation between the flavonoids content and associated markers was analyzed for genetic improvement studies on Tartary buckwheat. Method Sixty-two SSR primer pairs were used to assess the genetic diversity of 193 Tartary buckwheat germplasms. The SSR markers closely related to the total flavonoids content were selected by association analysis. Result The total flavonoids content of 193 Tartary buckwheat germplasms varied from 1.04% to 2.99% with a coefficient variation of 27.93%. Sixty-two pairs of SSR primers amplified 267 polymorphic sites with an average number of effective alleles of 2.45. The mean values of gene diversity, Shannon index, observed heterozygosity and polymorphism information content of SSR markers were 0.503, 0.942, 0.513 and 0.74, respectively. The population structure analysis divided the 193 germplasms into 3 subgroups. Five SSR markers were determined to be significantly associated with flavonoids content by the general linear model (GLM). The phenotypic variations ranged from 6.3% to 12.9% with TatG0124 at 12.9% and S6853 at 8.5% being the higher markers. Conclusion The germplasms used in this study were diverse in genetic variation and feasible for related genome-wide mapping on important traits of Tartary buckwheat. The loci, TatG0124 and S6853, appeared to closely associate with the flavonoids content of Tartary buckwheat and were considered to be potentially useful for breeding new varieties with high content of the functional components.
- Tartary buckwheat,
- SSR marker,
- flavonoids content,
- genetic diversity,
- population structure,
- correlation analysis

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References(30)

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