Agronomic Characteristics and ISSR Molecular Markers of <i>Auricularia cornea</i> Strains

KE Lina; HUANG Yining; YUAN BIN; ZHANG Zhihong; LIAN Yanping; WU Zhenqiang

doi:10.19303/j.issn.1008-0384.2021.07.006

Volume 36 Issue 7

Jul. 2021

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(7): 771-778

KE L N, HUANG Y N, YUAN B, et al. Agronomic Characteristics and ISSR Molecular Markers of Auricularia cornea Strains [J]. Fujian Journal of Agricultural Sciences，2021，36（7）：771−778 doi: 10.19303/j.issn.1008-0384.2021.07.006

Citation:

KE L N, HUANG Y N, YUAN B, et al. Agronomic Characteristics and ISSR Molecular Markers of Auricularia cornea Strains [J]. Fujian Journal of Agricultural Sciences，2021，36（7）：771−778 doi: 10.19303/j.issn.1008-0384.2021.07.006

Citation:

PDF( 738 KB)

Agronomic Characteristics and ISSR Molecular Markers of Auricularia cornea Strains

doi: 10.19303/j.issn.1008-0384.2021.07.006

1.
Zhangzhou Institute of Agricultural Sciences, Zhangzhou, Fujian　363005, China
2.
Zhangzhou Institute of Technology, Zhangzhou, Fujian　363000, China

Received Date: 2021-03-24
Rev Recd Date: 2021-05-18

Available Online: 2021-07-13

Publish Date: 2021-07-28

Abstract

Abstract

Objective Morphological and genetic characteristics of strains of Auricularia cornea were studied to facilitate new variety breeding and germplasm conservation. Method The agronomic properties and ISSR molecular markers of 8 major strains of cultivated or hybrid A. cornea collected from Fujian and other producing provinces in China were determined for comparison. Result The strains had significant morphological and genetic differences. Zhanger 43-28 and Strain 43012 showed the highest rate of mycelial growth with an average diameter of 11.8 cm and 11.3 cm, respectively, a thickness of 0.128 cm and 0.126 cm, respectively, and an output of 72.5 g·bag⁻¹ and 71.5 g·bag⁻¹, respectively, on earpieces. Za 10 had numerous earpieces with the fruiting body a chrysanthemum-like appearance. And the ventral surface of Strain CR5 was light reddish brown in color. The dried fruiting bodies of the 8 strains were either white or yellow on the back. Thus, Yonger 13, Strain 781, and Zhangping 42 were commercially categorized as yellow-backed and the other strains white-backed A. cornea. At 75% similarity on ISSR molecular markers, the 8 strains were clustered into Group 1 that consisted of Strain 781 and Yonger 13, the similarity of the two strains was 98%. Group 2 of Strain CR5, Group 3 of Zhanger 43-28 and Za 10, the similarity of the two strains was 76%. Group 4 of Sumao 3 and Strain 43012, the similarity of the two strains was 83% and Group 5 of Zhangping 42, Zhangping 42 and the remainders at 53%. Conclusion Combined the agronomic characteristics and ISSR clustering result, Yonger 13 and Strain 781 appeared phylogenetically closely related. They were same species but had been given different names. Strains Za 10, CR5, 43012 and Zhanger 4328 have their own advantages and can be used as parents of cross breeding.
- Auricularia cornea,
- agronomic characters,
- ISSR,
- genetic analysis

FullText(HTML)

References(20)

References

[1]	罗信昌, 陈士瑜. 中国菇业大典[M]. 北京: 清华大学出版社, 2010.
[2]	袁滨, 张金文, 柯丽娜, 等. 白背毛木耳菌株栽培对比试验 [J]. 北方园艺, 2012(21):131−134. YUAN B, ZHANG J W, KE L N, et al. Comparative test of cultivation technology on different Auricularia polytricha [J]. Northern Horticulture, 2012(21): 131−134.(in Chinese)
[3]	谭伟, 苗人云, 周洁, 等. 毛木耳栽培技术研究进展 [J]. 食用菌学报, 2018, 25(1):1−12. TAN W, MIAO R Y, ZHOU J, et al. Advances in cultivation techniques of Auricularia cornea [J]. Acta Edulis Fungi, 2018, 25(1): 1−12.(in Chinese)
[4]	郭金英, 史灵燕, 张慧, 等. 冀中南毛木耳栽培菌株的鉴定和优良菌株筛选 [J]. 北方园艺, 2019(14):129−134. GUO J Y, SHI L Y, ZHANG H, et al. Screening excellent strains and identification of Auricularia polytricha in the middle and south area of Hebei Province [J]. Northern Horticulture, 2019(14): 129−134.(in Chinese)
[5]	任梓铭. 毛木耳多孢杂交育种研究[D]. 长春: 吉林农业大学, 2017. REN Z M. Study on the multi spore hybridization of Auricularia cornea ehrenb[D]. Changchun: Jilin Agricultural University, 2017. (in Chinese).
[6]	巫鹏飞. 白背毛木耳的杂交育种[D]. 福州: 福建农林大学, 2014. WU P F. Cross breeding of Auricularia polytricha[D]. Fuzhou: Fujian Agriculture and Forestry University, 2014. (in Chinese).
[7]	孔祥会. 毛木耳种质资源综合评价研究[D]. 长春: 吉林农业大学, 2019. KONG X H. Study of comprehensive evaluation of germplasm resource on Auricularia cornea[D]. Changchun: Jilin Agricultural University, 2019. (in Chinese).
[8]	柯丽娜, 袁滨, 吴小平, 等. 4个毛木耳菌株的农艺性状及ITS评价分析 [J]. 福建农业学报, 2016, 31(4):366−370. KE L N, YUAN B, WU X P, et al. Agronomic traits and ITS characteristics of four Auricularia polytricha(Mont.)Sacc. Strains [J]. Fujian Journal of Agricultural Sciences, 2016, 31(4): 366−370.(in Chinese)
[9]	张丹. 毛木耳种质资源研究[D]. 雅安: 四川农业大学, 2005. ZHANG D. Studies on germplasm resources of Auricularia polytricha[D]. Yaan, China: Sichuan Agricultural University, 2005. (in Chinese).
[10]	林原, 陈剑, 赵光辉, 等. 7个秀珍菇菌株栽培特性及ISSR遗传分析 [J]. 福建农业学报, 2015, 30(4):339−343. doi: 10.3969/j.issn.1008-0384.2015.04.006 LIN Y, CHEN J, ZHAO G H, et al. Cultivation characteristics and ISSR genetic analysis on seven Pleurotus geesteranus strains [J]. Fujian Journal of Agricultural Sciences, 2015, 30(4): 339−343.(in Chinese) doi: 10.3969/j.issn.1008-0384.2015.04.006
[11]	陈影, 姚方杰, 张友民, 等. 黑木耳种质资源的农艺性状测试方法 [J]. 食药用菌, 2014, 22(3):153−154. CHEN Y, YAO F J, ZHANG Y M, et al. Methods for testing agronomic traits of germplasm resources of auricularia auriculata [J]. Edible and Medicinal Mushrooms, 2014, 22(3): 153−154.(in Chinese)
[12]	王关林. 植物基因工程[M]. 北京: 科学出版社, 2002.
[13]	ZIETKIEWICZ E, RAFALSKI A, LABUDA D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification [J]. Genomics, 1994, 20(2): 176−183. doi: 10.1006/geno.1994.1151
[14]	闫可, 尹勇刚, 傅常娥, 等. 桦褐孔菌菌株遗传多样性的ISSR分析 [J]. 食用菌学报, 2012, 19(2):26−30. doi: 10.3969/j.issn.1005-9873.2012.02.004 YAN K, YIN Y G, FU C E, et al. Genetic diversity among 21 Inonotus obliquus strains revealed by ISSR analysis [J]. Acta Edulis Fungi, 2012, 19(2): 26−30.(in Chinese) doi: 10.3969/j.issn.1005-9873.2012.02.004
[15]	陈世通, 李梦杰, 蒲敏, 等. ISSR分子标记鉴定香菇单孢杂交后代的研究 [J]. 江苏农业科学, 2012, 40(11):35−37. doi: 10.3969/j.issn.1002-1302.2012.11.012 CHEN S T, LI M J, PU M, et al. Identification of hybrid progeny of Lentinus edodes by SSR molecular markers [J]. Jiangsu Agricultural Sciences, 2012, 40(11): 35−37.(in Chinese) doi: 10.3969/j.issn.1002-1302.2012.11.012
[16]	贾定洪, 郑林用, 王波, 等. 22个毛木耳菌株的ISSR分析 [J]. 西南农业学报, 2010, 23(5):1595−1598. doi: 10.3969/j.issn.1001-4829.2010.05.044 JIA D H, ZHENG L Y, WANG B, et al. Analysis of 22 Auricularia polytricha strains with ISSR markers [J]. Southwest China Journal of Agricultural Sciences, 2010, 23(5): 1595−1598.(in Chinese) doi: 10.3969/j.issn.1001-4829.2010.05.044
[17]	袁滨, 张金文, 柯丽娜, 等. 毛木耳新菌株比较试验初报 [J]. 食用菌, 2012, 34(1):19−20, 24. doi: 10.3969/j.issn.1000-8357.2012.01.010 YUAN B, ZHANG J W, KE L N, et al. Preliminary report on screening of new strains of Auricularia auricula [J]. Edible Fungi, 2012, 34(1): 19−20, 24.(in Chinese) doi: 10.3969/j.issn.1000-8357.2012.01.010
[18]	沈天峰, 申进文, 王付才, 等. 平菇菌丝体生长速度与子实体产量的相关性研究 [J]. 中国食用菌, 2002, 21(4):18−19. doi: 10.3969/j.issn.1003-8310.2002.04.007 SHEN T F, SHEN J W, WANG F C, et al. Study on the correlation between mycelium growth rate and the fruiting body yield of Pleurotus [J]. Edible Fungi of China, 2002, 21(4): 18−19.(in Chinese) doi: 10.3969/j.issn.1003-8310.2002.04.007
[19]	司梦琦, 彭强, 许修宏. 不同黑木耳菌株在松木屑培养基上菌丝生长和子实体产量研究 [J]. 东北农业大学学报, 2009, 40(5):42−45. doi: 10.3969/j.issn.1005-9369.2009.05.009 SI M Q, PENG Q, XU X H. Study on mycelium growth and yield of different strains of Auricularia auricular in substrate of pine sawdust [J]. Journal of Northeast Agricultural University, 2009, 40(5): 42−45.(in Chinese) doi: 10.3969/j.issn.1005-9369.2009.05.009
[20]	SCHOUSTRA S, PUNZALAN D. Correlation of mycelial growth rate with other phenotypic characters in evolved genotypes of Aspergillus nidulans [J]. Fungal Biology, 2012, 116(5): 630−636. doi: 10.1016/j.funbio.2012.03.002