Effects of <i>Chenopodium ambrosioides</i> Essential Oil on Exoskeleton and Insecticide Permeation of <i>Plutella xylostella</i> Larvae

ZHAO Jianwei; CHEN Yixin; LIN Shuo; CHEN Yong; TIAN Houjun

doi:10.19303/j.issn.1008-0384.2021.06.011

Volume 36 Issue 6

Jun. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(6): 692-698

ZHAO J W, CHEN Y X, LIN S, et al. Effects of Chenopodium ambrosioides Essential Oil on Exoskeleton and Insecticide Permeation of Plutella xylostella Larvae [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：692−698 doi: 10.19303/j.issn.1008-0384.2021.06.011

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ZHAO J W, CHEN Y X, LIN S, et al. Effects of Chenopodium ambrosioides Essential Oil on Exoskeleton and Insecticide Permeation of Plutella xylostella Larvae [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：692−698 doi: 10.19303/j.issn.1008-0384.2021.06.011

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Effects of Chenopodium ambrosioides Essential Oil on Exoskeleton and Insecticide Permeation of Plutella xylostella Larvae

doi: 10.19303/j.issn.1008-0384.2021.06.011

Institute of Plant Protection, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fuzhou Scientific Observation and Experimental Station on Crop Pests, Ministry of Agriculture and Rural Affairs/East China Branch, National Center for Agricultural Biosafety Sciences, Fuzhou, Fujian　350013, China

Received Date: 2021-04-25
Rev Recd Date: 2021-05-20

Available Online: 2021-07-13

Publish Date: 2021-06-28

Abstract

Abstract

Objective Effects of Chenopodium ambrosioides (L.) essential oil on the exoskeleton structure and the insecticide permeation of Plutella xylostella (Linn.) were studied to determine if the insecticidal efficacy on the moth could be improved by treatment with the essential oil. Method Changes on the exoskeleton ultrastructure of diamondback moth (DBM, P. xylostella) larvae upon treatment of the wormseed essential oil (WEO) or Azone (laurocapram) were examined under a scanning electron microscope. The permeabilities of subsequent separate application of 3 insecticides into the larvae by percutaneous absorption and penetration were determined by GC-MS. Result Using distilled water as control and acetone for comparison, WEO or Azone treatment produced significant alterations on the spines and the epicuticle waxy layer structure of DBM. The hair-like piles became sparse, the cuticle surface smoothed, and the lower part of spines folded or cracked. Prolonged treatment significantly increased the penetration of the 3 subsequently applied insecticides through DBM exoskeleton. In 24h, the transdermal absorption rate of fipronil had reached 99.34%, which was significantly higher than control（55.12%）. Conclusion The enhancing effect of WEO pretreatment on the transdermal penetration of subsequently applied insecticide on DBM was similar to that of Azone. The enhancement on fipronil penetration was also found on trichlorfon and chlorpyrifos permeation. Similar results were, therefore, expected for the cases involving other organophosphorus, phenylpyrazole, and thiophosphate insecticides.
- Plutella xylostlla (Linn.),
- Chenopodium ambrosioides L.,
- essential oil,
- transdermal absorption,
- insecticides

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