HPLC-MS/MS Determination of Melatonin in Brown Planthopper at Growth Stages

SHI Longqing; XIE Zhenxing; JIANG Zhaowei; DONG Meng; ZHAN Zhixiong

doi:10.19303/j.issn.1008-0384.2021.06.010

Volume 36 Issue 6

Jun. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(6): 687-691

SHI L Q, XIE Z X, JIANG Z W, et al. HPLC-MS/MS Determination of Melatonin in Brown Planthopper at Growth Stages [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：687−691 doi: 10.19303/j.issn.1008-0384.2021.06.010

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SHI L Q, XIE Z X, JIANG Z W, et al. HPLC-MS/MS Determination of Melatonin in Brown Planthopper at Growth Stages [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：687−691 doi: 10.19303/j.issn.1008-0384.2021.06.010

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HPLC-MS/MS Determination of Melatonin in Brown Planthopper at Growth Stages

doi: 10.19303/j.issn.1008-0384.2021.06.010

Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350018, China

Received Date: 2020-12-17
Rev Recd Date: 2021-03-30

Available Online: 2021-07-13

Publish Date: 2021-06-28

Abstract

Abstract

Objective Melatonin contents in brown planthopper, Nilaparvata lugens (Stl), at various developmental stages were determined. Method HPLC-MS/MS was used to measure the melatonin contents in the 1^st to the 5^th instar nymphs as well as female and male macropterous and brachypterous adults of the insect. Result At nymph stage, N. lugens had the lowest average melatonin content of 378.5 pg·g⁻¹ in the 1^st and the 2^nd instars, the highest of 856.6 pg·g⁻¹ in the 3^rd instar, 810.7 pg·g⁻¹ in the 4^th instar, and 574.5 pg·g⁻¹ in the 5^th instar. Adult N. lugens differed significantly on melatonin contents between male and female but not between the macropterous and brachypterous wing types. The male of macropterous and brachypterous wing types contained 978.3 pg·g⁻¹ and 969.6 pg·g⁻¹ melatonin, respectively, which were extremely significantly higher than the female counterparts at 76.1 pg·g⁻¹ and 176.8 pg·g⁻¹, respectively. Conclusion The melatonin contents in N. lugens as nymphs and adults were determined.
- Nilaparvata lugens (Stål),
- melatonin,
- endogenous hormone,
- sex difference,
- rice pest,
- wing type

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References(22)

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