Preparation and Functionalities of Bacillus Agents Cultured on Soymilk Wastewater

GUAN Xuefang; WANG Qi; ZHENG Qi; LIN Bin; HUANG Juqing

doi:10.19303/j.issn.1008-0384.2021.06.008

Volume 36 Issue 6

Jun. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(6): 670-678

GUAN X F, WANG Q, ZHENG Q, et al. Preparation and Functionalities of Bacillus Agents Cultured on Soymilk Wastewater [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：670−678 doi: 10.19303/j.issn.1008-0384.2021.06.008

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GUAN X F, WANG Q, ZHENG Q, et al. Preparation and Functionalities of Bacillus Agents Cultured on Soymilk Wastewater [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：670−678 doi: 10.19303/j.issn.1008-0384.2021.06.008

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Preparation and Functionalities of Bacillus Agents Cultured on Soymilk Wastewater

doi: 10.19303/j.issn.1008-0384.2021.06.008

GUAN Xuefang^{1, 2
,},
WANG Qi^{1, 2},
ZHENG Qi¹,
LIN Bin^{1
,
,},
HUANG Juqing^{1, 2}

1.
Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 　350003, China
2.
Fujian Key Laboratory of Agricultural Produce (Food) Processing, Fuzhou, Fujian 　350003, China

Received Date: 2020-11-15
Rev Recd Date: 2021-04-29

Available Online: 2021-07-13

Publish Date: 2021-06-28

Abstract

Abstract

Objective Fermentation processes utilizing soymilk processing wastewater to prepare feed additives with a high viable bacteria count and desirable functionalities were tested for applications. Method Bacillus subtilis SB13 and B. licheniformis BL14 were cultured in the wastewater. Medium formulation and process optimization were conducted with a single factor experiment determined by the count of viable bacteria in the resulting preparations. Functions as an additive for chicken, pig, and cattle feeds of the preparations was evaluated by an in vitro simulated digestion and a DNS method. Result Both SB13 and BL14 grew normally in the soymilk wastewater. The optimized culture processes for SB13 and BL14 included sporulation temperatures at 35 ℃ for 72 h and 48 h, respectively. To facilitate dehydrating the fermentation broths, a protective agent of 10% skim milk powder was added in the medium for SB13 and 4% sucrose for BL14, as well as a carrier of 10% wheat bran for SB13 and 10% starch for BL14. After completion of the fermentation, the broths were freeze-dried and kept in sealed containers prior to functionality tests. The dried SB13 agent showed a viable bacteria count of 6.40×10⁸ cfu·mL⁻¹ and a survival rate at 97.71%, while BL14 a count of 9.80×10⁸ cfu·mL⁻¹ and a survival rate at 94.69%. Upon the artificial gastric juice and pancreatic juice treatments for 2 h, the two preparations had survived counts ranged from 2.55×10⁷ to 13.10×10⁷ cfu·mL⁻¹. The endoglucanase activity of the preparations was more than 39% retained after the gastric juice treatment and 72% after the pancreatic juice treatment. Both agents displayed significant glycation on feed, and BL14 exhibited a significant inhibitory effect on Staphylococcus aureus. Conclusion Utilizing the wastewater from soymilk processing to culture B. subtilis SB13 and B. licheniformis BL14 resulted in preparations that could become a functional additive to forages for livestock and poultry industry. The process was cost effective and environmentally friendly.
- Bacillus preparation,
- carrier,
- protective agent,
- endoglucanase,
- soymilk processing wastewater

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References(35)

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