Agronomic and Heavy Metal Reducing Properties of <sup>60</sup>Co-induced <i>Agaricus blazei</i> Mutant

CHEN Hua; YE Jing; LIN Yi; WANG Yixiang; LIU Penghu

doi:10.19303/j.issn.1008-0384.2021.06.005

Volume 36 Issue 6

Jun. 2021

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(6): 651-657

CHEN H, YE J, LIN Y, et al. Agronomic and Heavy Metal Reducing Properties of 60Co-induced Agaricus blazei Mutant [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：651−657 doi: 10.19303/j.issn.1008-0384.2021.06.005

Citation:

CHEN H, YE J, LIN Y, et al. Agronomic and Heavy Metal Reducing Properties of ⁶⁰Co-induced Agaricus blazei Mutant [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：651−657 doi: 10.19303/j.issn.1008-0384.2021.06.005

Citation:

PDF( 610 KB)

Agronomic and Heavy Metal Reducing Properties of ⁶⁰Co-induced Agaricus blazei Mutant

doi: 10.19303/j.issn.1008-0384.2021.06.005

CHEN Hua^{1, 2
,},
YE Jing^{1, 2},
LIN Yi^{1, 2},
WANG Yixiang^{1, 2},
LIU Penghu^{2, 3
,
,}

1.
Institute of Agricultural Ecology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Fujian Key Laboratory of Agro-ecological Processes in Red Soil Hilly Region, Fuzhou, Fujian　350003, China
3.
China National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2021-02-23
Rev Recd Date: 2021-04-30

Available Online: 2021-06-06

Publish Date: 2021-06-28

Abstract

Abstract

Objective As an edible and medicinal fungus, Agaricus blazei Murrill is prone to accumulate heavy metals such as cadmium in production, resulting in excessive cadmium and other heavy metals. Breeding new varieties of Agaricus blazei Murrill with low cadmium and high quality is a scientific research target of great concern in the industry. Methods The J1 cultivar of A. blazei was exposed to ⁶⁰Co radiation to obtain J37. The main agronomic traits, yield, and contents of amino acids, cadmium, lead, arsenic, and mercury in fruitarian bodies of J1 and J37 in the 1st to 4th tides were systematically compared. The effects of different growth algebras on fruitbody yield of Agaricus blazei Murrill J1 and J37 were also observed. Result (1) The flower weight, cover weight, cover thickness, cover diameter, cover height, stem weight, stem diameter and other agronomic traits of new strain J37 were significantly or extremely significantly higher than those of control (J1), while stem length was significantly lower than that of J1, and essential amino acid content was extremely significantly higher than that of J1; (2) Compared with J1, the yield of J37 in 1-6 generation (M1-6) was higher than that of J1, and the growth rate of fruite body yield in M1, M2, M3, M4, M5 and M6 generations of J37 was 30.11%, 33.58%, 39.46%, 39.45%, 34.14% and 33.53%, respectively, higher than that of J1. The difference reached extremely significant level; (3) Compared with J1, the contents of CD, Pb, Hg and AS in J37 fruite body decreased by 48.09%, 58.57%, 26.03% and 75.26%, respectively, and the differences were extremely significant. f A. blazei. The irradiation treatment appeared to have a potential for mushroom breeding. Conclusion The new agaricus blazei strain J37 showed good yield, high quality and safety.
- Agaricus blazei,
- ⁶⁰Co,
- radiation mutation,
- new strain,
- agronomic traits,
- heavy metals,
- effect

FullText(HTML)

References(24)

References

[1]	岳丽玲, 张巍, 刘丹, 等. 姬松茸药用价值研究进展 [J]. 中国药房, 2011, 22(43):4116−4118. YUE L L, ZHANG W, LIU D, et al. Research progress on medicinal value of Agaricus blazei Murrill [J]. China Pharmacy, 2011, 22(43): 4116−4118.(in Chinese)
[2]	江枝和. 姬松茸 [J]. 中国食用菌, 1993(3):封 4. JIANG Z H. Agaricus blazei Murrill [J]. Edible Fungi of China, 1993(3): 封 4.(in Chinese)
[3]	徐丽红, 何莎莉, 吴应淼, 等. 姬松茸对有害重金属镉的吸收富集规律及控制技术研究 [J]. 中国食品学报, 2010, 10(4):152−158. doi: 10.3969/j.issn.1009-7848.2010.04.024 XU L H, HE S L, WU Y M, et al. Investigation of the law and control technique of cadmium absorption and accumulation of Agaricus blazei murrill [J]. Journal of Chinese Institute of Food Science and Technology, 2010, 10(4): 152−158.(in Chinese) doi: 10.3969/j.issn.1009-7848.2010.04.024
[4]	黄建成, 李开本, 何锦星, 等. 姬松茸镉累积特性研究Ⅰ. 培养料镉污染对子实体的效应 [J]. 福建农业学报, 2006, 21(3):240−243. doi: 10.3969/j.issn.1008-0384.2006.03.014 HUANG J C, LI K B, HE J X, et al. Cadmium accumulated characteristic in Agaricus Blazei Murrill Ⅰ. Effects of Cadmium pollution in medium materials on fruit body [J]. Fujian Journal of Agricultural Sciences, 2006, 21(3): 240−243.(in Chinese) doi: 10.3969/j.issn.1008-0384.2006.03.014
[5]	黄建成, 余应瑞, 应正河, 等. 姬松茸镉累积特性研究: Ⅱ镉胁迫对菌丝及子实体生长发育的影响 [J]. 农业环境科学学报, 2008, 27(1):78−81. doi: 10.3321/j.issn:1672-2043.2008.01.013 HUANG J C, YU Y R, YING Z H, et al. Accumulation characteristic of cadmium in Agaricus blazei murrill Ⅱ. effects of Cd stress on growth and development of mycelium and fruit body [J]. Journal of Agro-Environment Science, 2008, 27(1): 78−81.(in Chinese) doi: 10.3321/j.issn:1672-2043.2008.01.013
[6]	杨春香, 林新坚, 林跃鑫. 镉(Cd)对姬松茸菌丝生长的影响 [J]. 中国食用菌, 2004, 23(4):36−38, 52. doi: 10.3969/j.issn.1003-8310.2004.04.019 YANG C X, LIN X J, LIN Y X. Effects of cadmium on the mycelium growth of Agaricus blazei murill [J]. Edible Fungi of China, 2004, 23(4): 36−38, 52.(in Chinese) doi: 10.3969/j.issn.1003-8310.2004.04.019
[7]	JIANG Z H, WENG B Q, HUANG T J, et al. Nutrient value protein of mutant J₃ of agarious blazei by irradiation [J]. Acta Agriculturae Nucleatae Sinica, 2003, 17(1):20−23.
[8]	江枝和, 翁伯琦, 雷锦桂, 等. 姬松茸⁶⁰Co辐射新菌株J₅的营养成分、重金属含量与农药残留分析及其安全性评价 [J]. 热带作物学报, 2010, 31(10):1702−1705. doi: 10.3969/j.issn.1000-2561.2010.10.009 JIANG Z H, WENG B Q, LEI J G, et al. Analysis of heavy metals and pesticide residues in strain J₅ Agaricus blazei murril radiated by ⁶⁰Co [J]. Chinese Journal of Tropical Crops, 2010, 31(10): 1702−1705.(in Chinese) doi: 10.3969/j.issn.1000-2561.2010.10.009
[9]	刘朋虎, 陈爱华, 江枝和, 等. 姬松茸“福姬J77”新菌株选育研究 [J]. 福建农业学报, 2012, 27(12):1333−1338. doi: 10.3969/j.issn.1008-0384.2012.12.013 LIU P H, CHEN A H, JIANG Z H, et al. A brief report on breeding of Agaricus blazei strain “FUJI J77” [J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1333−1338.(in Chinese) doi: 10.3969/j.issn.1008-0384.2012.12.013
[10]	孙玲, 刘利平, 徐婉茹, 等. 物理诱变在药食用菌育种中的应用研究进展 [J]. 安徽农业科学, 2018, 46(14):29−33, 153. doi: 10.3969/j.issn.0517-6611.2018.14.009 SUN L, LIU L P, XU W R, et al. Research progress on physical mutation breeding of edible and medical fungi [J]. Journal of Anhui Agricultural Sciences, 2018, 46(14): 29−33, 153.(in Chinese) doi: 10.3969/j.issn.0517-6611.2018.14.009
[11]	李刚, 清源, 张守武. 香菇原生质体诱变育种探析 [J]. 信息周刊, 2018(23):1. LI G, QING Y, ZHANG S W. Mutation breeding of Lentinus edodes protoplasts [J]. Information Week, 2018(23): 1.(in Chinese)
[12]	刘昆昂, 刘萌, 张根伟, 等. 金针菇遗传育种研究进展 [J]. 江苏农业科学, 2019, 47(14):18−22. LIU K N, LIU M, ZHANG G W, et al. Research progress on genetic breeding of Flammulina velutipes [J]. Jiangsu Agricultural Sciences, 2019, 47(14): 18−22.(in Chinese)
[13]	李永文, 刘阳, 张义奇, 等. 平菇菌株诱变处理及应用初探 [J]. 安徽农业科学, 2007, 35(21):6427−6428. doi: 10.3969/j.issn.0517-6611.2007.21.044 LI Y W, LIU Y, ZHANG Y Q, et al. Primary discussion on the mutagenesis treatment of Pleurotus ostreatus strain and its application [J]. Journal of Anhui Agricultural Sciences, 2007, 35(21): 6427−6428.(in Chinese) doi: 10.3969/j.issn.0517-6611.2007.21.044
[14]	董玉玮, 苗敬芝, 曹泽虹, 等. 紫外诱变赤灵芝原生质体选育高产有机锗菌株的研究 [J]. 食品科学, 2009, 30(15):188−192. doi: 10.3321/j.issn:1002-6630.2009.15.043 DONG Y W, MIAO J Z, CAO Z H, et al. UV mutagenization of protoplast for screening and breeding of High-yield Organogemanium-producing strains of Ganoderma lucidum [J]. Food Science, 2009, 30(15): 188−192.(in Chinese) doi: 10.3321/j.issn:1002-6630.2009.15.043
[15]	夏志兰, 艾辛, 姜性坚. ⁶⁰Coγ射线对杏鲍菇菌丝的诱变效应 [J]. 激光生物学报, 2004, 13(4):298−301. doi: 10.3969/j.issn.1007-7146.2004.04.013 XIA Z L, AI X, JIANG X J. The effects of Pleurotus eryngii irradiated by ⁶⁰Coγ ray [J]. Acta Laser Biology Sinica, 2004, 13(4): 298−301.(in Chinese) doi: 10.3969/j.issn.1007-7146.2004.04.013
[16]	刘成荣, 柯晓青. 真姬菇诱变菌株的选育及其发酵条件研究 [J]. 福建农业学报, 2008, 23(4):462−465. doi: 10.3969/j.issn.1008-0384.2008.04.024 LIU C R, KE X Q. Mutation and fermentation of hypsizigus marmoreus [J]. Fujian Journal of Agricultural Sciences, 2008, 23(4): 462−465.(in Chinese) doi: 10.3969/j.issn.1008-0384.2008.04.024
[17]	马三梅, 王永飞, 亦如瀚. 食用菌育种的研究进展 [J]. 西北农林科技大学学报(自然科学版), 2004, 32(4):108−112. MA S M, WANG Y F, YI R H. Review of edible fungi breeding [J]. Journal of Northwest Sci-Tech University of Agriculture and Forestry, 2004, 32(4): 108−112.(in Chinese)
[18]	桂明英, 王刚, 郭永红, 等. 食用菌育种技术的研究进展 [J]. 中国食用菌, 2006, 25(5):3−5. doi: 10.3969/j.issn.1003-8310.2006.05.001 GUI M Y, WANG G, GUO Y H, et al. Research advancement of breeding technique about edible fungi [J]. Edible Fungi of China, 2006, 25(5): 3−5.(in Chinese) doi: 10.3969/j.issn.1003-8310.2006.05.001
[19]	翁伯琦, 江枝和, 黄挺俊, 等. 姬松茸⁶⁰Co辐射菌株J3若干特性研究 [J]. 中国农业科学, 2003, 36(9):1065−1070. doi: 10.3321/j.issn:0578-1752.2003.09.014 WENG B Q, JIANG Z H, HUANG T J, et al. Study on several characteristics of strain J3 Agaricus blazei murril irradiated by ⁶⁰Co [J]. Scientia Agricultura Sinica, 2003, 36(9): 1065−1070.(in Chinese) doi: 10.3321/j.issn:0578-1752.2003.09.014
[20]	翁伯琦, 江枝和, 肖淑霞, 等. 姬松茸⁶⁰Co辐射新菌株J₃营养成分与农药残留分析 [J]. 农业环境科学学报, 2011, 30(2):244−248. WENG B Q, JIANG Z H, XIAO S X, et al. Analysis on nutrients and pesticide residues in strain J3 Agaricus blazei murill irradiated by 60Co [J]. Journal of Agro-Environment Science, 2011, 30(2): 244−248.(in Chinese)
[21]	张卉, 李长彪, 陈明波, 等. 原生质体紫外诱变选育姬松茸新菌株 [J]. 微生物学杂志, 2004, 24(6):56−57. doi: 10.3969/j.issn.1005-7021.2004.06.016 ZHANG H, LI C B, CHEN M B, et al. Mutagenesis breeding new strain of jisongrong mushroom (Agaricus blazei) by UV [J]. Journal of Microbiology, 2004, 24(6): 56−57.(in Chinese) doi: 10.3969/j.issn.1005-7021.2004.06.016
[22]	颜丽君, 郑焕春. 姬松茸⁶⁰Co-γ射线辐照诱变育种试验初报 [J]. 中国食用菌, 2008, 27(5):19−21. doi: 10.3969/j.issn.1003-8310.2008.05.005 YAN L J, ZHENG H C. A preliminary report on exposuring breeding of Agaricus blazei murrill with ⁶⁰Co-γ radiation [J]. Edible Fungi of China, 2008, 27(5): 19−21.(in Chinese) doi: 10.3969/j.issn.1003-8310.2008.05.005
[23]	刘朋虎, 李波, 江枝和, 等. 镉对姬松茸农艺性状及矿物质元素吸收的影响 [J]. 农业环境科学学报, 2018, 37(1):58−63. doi: 10.11654/jaes.2017-1524 LIU P H, LI B, JIANG Z H, et al. Effects of cadmium(Cd) in different concentrations on agronomic traits and mineral elements absorption of Agaricus brasiliensis [J]. Journal of Agro-Environment Science, 2018, 37(1): 58−63.(in Chinese) doi: 10.11654/jaes.2017-1524
[24]	刘朋虎, 李波, 江枝和, 等. 姬松茸菌株J1与J77镉富集差异及生理响应机制 [J]. 农业环境科学学报, 2017, 36(5):863−868. doi: 10.11654/jaes.2017-0073 LIU P H, LI B, JIANG Z H, et al. Comparison and physiological mechanisms of cadmium (Cd) accumulation by strain J1 and mutant J77 of Agaricus brasiliensis [J]. Journal of Agro-Environment Science, 2017, 36(5): 863−868.(in Chinese) doi: 10.11654/jaes.2017-0073