Endophytic Bacteria for Biocontrol of Copper Blight on <i>Cunninghamia lanceolate</i>

LIU Yujing; ZENG Hualong; LIANG Weikang; LU Meng; ZHU Weiyao; ZHANG Lei; SONG Zhang; ZHANH Qinghua

doi:10.19303/j.issn.1008-0384.2021.05.009

Volume 36 Issue 5

May 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(5): 556-563

LIU Y J, ZENG H L, LIANG W K, et al. Endophytic Bacteria for Biocontrol of Copper Blight on Cunninghamia lanceolate [J]. Fujian Journal of Agricultural Sciences，2021，36（5）：556−563 doi: 10.19303/j.issn.1008-0384.2021.05.009

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LIU Y J, ZENG H L, LIANG W K, et al. Endophytic Bacteria for Biocontrol of Copper Blight on Cunninghamia lanceolate [J]. Fujian Journal of Agricultural Sciences，2021，36（5）：556−563 doi: 10.19303/j.issn.1008-0384.2021.05.009

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Endophytic Bacteria for Biocontrol of Copper Blight on Cunninghamia lanceolate

doi: 10.19303/j.issn.1008-0384.2021.05.009

1.
Institute of Forest Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Key Lab of Natural Pesticides and Chemical Biology, Ministry of Education/South China Agricultural University, Guangzhou, Guangdong　510642, China
3.
Institute of Plant Protection, Sichuan Academy of Agriculture, Chengdu, Sichuan　610066, China

More Information

Corresponding author: 张清华（1986-），男，博士，讲师，研究方向：森林病理学（E-mail：fzhangqinghua@fafu.edu.cn）
Received Date: 2020-11-09
Rev Recd Date: 2021-01-27

Available Online: 2021-04-20

Publish Date: 2021-05-31

Abstract

Abstract

Objective Endophytic bacteria that could effectively control the copper blight, one of the most significant diseases that seriously infects the seedlings and saplings, of Chinese fir (Cunninghamia lanceolate) were investigated. Methods Candidate endophytic bacteria were cultured and isolated from leaves of Chinese fir followed by morphological, physiological, and biochemical analyses as well as 16S rRNA sequencing for identification. Pathogen inhibition against Pestalotiopsis apiculatus by the sterile fermentation filtrate of potential specimens was tested on toxic plate and Oxford cup and observed under a microscope. Effectiveness of the disease control by the selected bacteria was further verified in vitro on fir leaves. Results The endophytic bacterium coded SM-1 showed a significant antagonistic effect against P. apiculatus. It was isolated and identified as Bacillus atrophaeus. On the toxic plate and Oxford cup tests, the SM-1 fermentation filtrate yielded a P. apiculatus inhibition rate of 58.80%. Morphologically, the bacterium inhibited the mycelial growth and caused cellular swelling that changed the cells spherical in appearance of the pathogen. The efficacy of the SM-1 filtrate on copper blight on the fir leaves was 95% with significantly reduced disease severity (P＜0.01). Conclusion The SM-1, identified as B. atrophaeus, appeared to be a potential biocontrol agent to deter copper blight on Chinese fir.
- Bacillus atrophaeus,
- copper blight of Chinese fir,
- endophytic bacteria,
- biological control

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References(22)

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