Cloning and Bioinformatics of Chalcone Synthase Gene of <i>Moringa oleifera</i>

LIN Ling; FANG Jianchao; CHEN Guanshui; AI Yufang

doi:10.19303/j.issn.1008-0384.2021.05.008

Volume 36 Issue 5

May 2021

Turn off MathJax

Article Contents

Abstract

References

Fujian Journal of Agricultural Sciences > 2021 > 36(5): 549-555. > DOI: 10.19303/j.issn.1008-0384.2021.05.008

LIN L, FANG J C, , et al. Cloning and Bioinformatics of Chalcone Synthase Gene of Moringa oleifera [J]. Fujian Journal of Agricultural Sciences，2021，36（5）：549−555. DOI: 10.19303/j.issn.1008-0384.2021.05.008

Citation:

PDF (1050 KB)

Cloning and Bioinformatics of Chalcone Synthase Gene of Moringa oleifera

1.
Department of Pharmacy, Fujian Health College, Fuzhou, Fujian　350101, China
2.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

More Information

Received Date: January 20, 2021
Revised Date: February 26, 2021
Available Online: April 19, 2021

Graphical Abstract

Abstract

Abstract

Objective The open reading frame and gDNA of the gene associated with the chalcone synthase (CHS), the first key enzyme in the flavonoid biosynthetic pathway in Moringa oleifera, were cloned for a bioinformatic analysis.
Method Primers were designed according to the M. oleifera genome in NCBI database. The leaf cDNA and genomic DNA were used as templates to amplify the MoCHS1 with PCR for a subsequent bioinformatic analysis. DNAMAN 9.0 and MEGA 10.0 were used for the multiple sequence alignment and phylogenetic tree construction.
Result The ORF of MoCHS1 was 1 185 bp encoded 394 amino acids. The genomic DNA spanned 1 387 bp containing 2 exons and 1 intron. MoCHS1 was a stable hydrophilic protein with a structure of 45.43% α helix and 31.98% random coil. The gene possessed conserved sequence as well as enzymatic site residues of the CHS gene superfamily, which included 7 amino acid residues of the cyclization pocket, the Cys-His-Asn catalytic triad sites, and the family signatures of CHSs (‘RLMMYQQGCFAGGTVLR’ and ‘GVLFGFGPGL’) sharing a high similarity with the CHS proteins of other species. The phylogenetic tree of MoCHS1 closely related to and in the same clade with the CHS of Carica papaya.
Conclusion MoCHS1 was successfully isolated from M. oleifera sharing a typical conserved structure of the CHS gene family. The results provided crucial information for further study on the genes related to the flavonoid metabolic pathway as well as the expression regulation and evolution of the CHS gene family in M. oleifera.
- Moringa oleifera,
- flavonoid,
- chalcone synthase,
- bioinformatic analysis

FullText(HTML)

References (19)

References

[1]	CHEN G L, XU Y B, WU J L, et al. Hypoglycemic and hypolipidemic effects of Moringa oleifera leaves and their functional chemical constituents [J]. Food Chemistry, 2020, 333: 127478. DOI: 10.1016/j.foodchem.2020.127478
[2]	周滢, 梁敏丽, 解雅倩, 等. 辣木籽总黄酮提取工艺优化 [J]. 广东化工, 2020(20):18−20. DOI: 10.3969/j.issn.1007-1865.2020.20.010 ZHOU Y, LIANG M L, XIE Y Q, et al. To Optimize the Extraction Technology of Total Flavonoids from Moringa Oleifera Seed [J]. Guangdong Chemical Industry, 2020(20): 18−20.(in Chinese) DOI: 10.3969/j.issn.1007-1865.2020.20.010
[3]	王玲玲, 边祥雨, 高蔚娜, 等. 辣木叶总黄酮提取物抗疲劳功效的研究 [J]. 营养学报, 2020, 42(1):68−77. DOI: 10.3969/j.issn.0512-7955.2020.01.014 WANG L L, BIAN X Y, GAO W N, et al. Anti-fatigue effect of total flavonoids extract from Moringa oleifera leaves [J]. Acta Nutrimenta Sinica, 2020, 42(1): 68−77.(in Chinese) DOI: 10.3969/j.issn.0512-7955.2020.01.014
[4]	PASHA S N, SHAFI K M, JOSHI A G, et al. The transcriptome enables the identification of candidate genes behind medicinal value of Drumstick tree (Moringa oleifera) [J]. Genomics, 2020, 112(1): 621−628. DOI: 10.1016/j.ygeno.2019.04.014
[5]	许明, 林世强, 倪冬昕, 等. 藤茶查尔酮合成酶基因AgCHS1的克隆及功能鉴定 [J]. 中国农业科学, 2020, 53(24):5091−5103. DOI: 10.3864/j.issn.0578-1752.2020.24.012 XU M, LIN S Q, NI D X, et al. Cloning and function characterization of chalcone synthase gene AgCHS1 in Ampelopsis grossedentata [J]. Scientia Agricultura Sinica, 2020, 53(24): 5091−5103.(in Chinese) DOI: 10.3864/j.issn.0578-1752.2020.24.012
[6]	TOHGE T, DE SOUZA L P, FERNIE A R. Current understanding of the pathways of flavonoid biosynthesis in model and crop plants [J]. Journal of Experimental Botany, 2017, 68(15): 4013−4028. DOI: 10.1093/jxb/erx177
[7]	张声祥, 施圆圆, 王晨凯, 等. 异叶天南星查尔酮合成酶和异构酶基因的克隆及蛋白的结构性质分析 [J]. 中国中药杂志, 2019, 44(9):1799−1807. ZHANG S X, SHI Y Y, WANG C K, et al. Cloning and characterization of Chalcone synthase and Chalcone isomerase genes in Arisaema heterophyllum [J]. China Journal of Chinese Materia Medica, 2019, 44(9): 1799−1807.(in Chinese)
[8]	CHEN S, PAN X H, LI Y T, et al. Identification and characterization of Chalcone synthase gene family members in Nicotiana tabacum [J]. Journal of Plant Growth Regulation, 2017, 36(2): 374−384. DOI: 10.1007/s00344-016-9646-6
[9]	ANGURAJ V A K, KRYSIAK K, TIAN G, et al. Genome-wide identification and localization of chalcone synthase family in soybean (Glycine max [L]Merr) [J]. BMC Plant Biology., 2018, 18(1): 325. DOI: 10.1186/s12870-018-1569-x
[10]	KODURI P K H, GORDON G S, BARKER E I, et al. Genome-wide analysis of the Chalcone synthase superfamily genes of Physcomitrella patens [J]. Plant Molecular Biology, 2010, 72(3): 247−263. DOI: 10.1007/s11103-009-9565-z
[11]	ABE I, MORITA H. Structure and function of the chalcone synthase superfamily of plant type III polyketide synthases [J]. Natrual Procuct Reports, 2010, 27(6): 809−838. DOI: 10.1039/b909988n
[12]	WANG C H, ZHI S, LIU C Y, et al. Isolation and characterization of a novel Chalcone synthase gene family from mulberry [J]. Plant Physiology and Biochemistry, 2017, 115: 107−118. DOI: 10.1016/j.plaphy.2017.03.014
[13]	LIOU G, CHIANG Y C, WANG Y, et al. Mechanistic basis for the evolution of Chalcone synthase catalytic cysteine reactivity in land plants [J]. The Journal of Biological Chemistry, 2018, 293(48): 18601−18612. DOI: 10.1074/jbc.RA118.005695
[14]	康亚兰, 裴瑾, 刘薇, 等. 红花查尔酮合成酶基因的克隆、生物信息学分析及表达 [J]. 中草药, 2014, 45(16):2385−2389. DOI: 10.7501/j.issn.0253-2670.2014.16.020 KANG Y L, PEI J, LIU W, et al. Cloning, bioinformatic analysis, and expression of Chalcone synthase gene in Carthamus tinctorius [J]. Chinese Traditional and Herbal Drugs, 2014, 45(16): 2385−2389.(in Chinese) DOI: 10.7501/j.issn.0253-2670.2014.16.020
[15]	关淑文, 王毅, 郝佳波, 等. 竹叶花椒查尔酮合成酶基因克隆与表达 [J]. 分子植物育种, 2020, 18(16):5300−5305. GUAN S W, WANG Y, HAO J B, et al. Cloning and expression of Chalcone synthase gene from Zanthoxylum armatum [J]. Molecular Plant Breeding, 2020, 18(16): 5300−5305.(in Chinese)
[16]	孙朝霞, 侯思宇, 郭彬, 等. 苦荞查尔酮合成酶基因序列特征及分子进化分析 [J]. 分子植物育种, 2014, 12(4):772−779. SUN Z X, HOU S Y, GUO B, et al. Characterize of sequences and molecular evolution analysis of Chalcone synthase in Fagopyrum tartaricum [J]. Molecular Plant Breeding, 2014, 12(4): 772−779.(in Chinese)
[17]	YAHYAA M, ALI S, DAVIDOVICH-RIKANATI R, et al. Characterization of three chalcone synthase-like genes from apple (Malus × domestica Borkh.) [J]. Phytochemistry., 2017, 140: 125−133. DOI: 10.1016/j.phytochem.2017.04.022
[18]	HAN Y Y, ZHAO W W, WANG Z C, et al. Molecular evolution and sequence divergence of plant Chalcone synthase and Chalcone synthase-Like genes [J]. Genetica, 2014, 142(3): 215−225. DOI: 10.1007/s10709-014-9768-3
[19]	SANJARI S, SHOBBAR Z S, EBRAHIMI M, et al. Chalcone synthase genes from milk thistle (Silybum marianum): Isolation and expression analysis [J]. Journal of Genetics, 2015, 94(4): 611−617. DOI: 10.1007/s12041-015-0560-7

[1]	CHEN Han, XU Hongguo, WANG Zhigang, XU Weihui, GE Xiaoqi, QI Hongying. Identification and Bioinformatics of PEBP Family in Hemp[J]. Fujian Journal of Agricultural Sciences, 2022, 37(8): 1016-1024. DOI: 10.19303/j.issn.1008-0384.2022.008.007
[2]	DONG Keqing, WANG Leili, LIU Qingqing, ZHANG Yanling, LI Anran, TONG Zhenhan, Dong Zhe, WANG Cuiling. Bioinformatics on Structure and Functions of ZmCOL3-encoded Protein[J]. Fujian Journal of Agricultural Sciences, 2021, 36(9): 999-1006. DOI: 10.19303/j.issn.1008-0384.2021.09.002
[3]	CHEN Yi-ting, LAI Rui-lian, FENG Xin, GAO Min-xia, ZHANG Chang-he, WU Ru-jian. Codon Usage Bias of Chalcone Synthase Gene CHS in Kiwifruit[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1213-1217. DOI: 10.19303/j.issn.1008-0384.2017.011.009
[4]	XIONG Gui-hong, LIU Xiao-juan, YANG Liang, WU Zu-jian. Subcellular Localization Analysis of Rice Protein Kinase Gene OsCIPK5 and Generation of Its RNAi Transgenic Plants[J]. Fujian Journal of Agricultural Sciences, 2017, 32(4): 353-358. DOI: 10.19303/j.issn.1008-0384.2017.04.001
[5]	YIN Ya-jun, ZHANG Tao, WANG Ling, LU Hong-zhao, DU Wei-li. Cloning and Bioinformatic Analysis of Anthocyanidin Synthase Gene from Hanzhong Black Rice[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 124-129. DOI: 10.19303/j.issn.1008-0384.2017.02.004
[6]	LIU Wei-hua, LIN Zheng-chun, FENG Xin, LAI Zhong-xiong. Cloning and Bioinformatic Analysis of HOS1 Promoter from the 'Tianbao' Banana (Musa acuminata. AAA group)[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 820-825. DOI: 10.19303/j.issn.1008-0384.2016.08.006
[7]	KONG Xiang-rui, YANG Jun, WANG Rang-jian. A Bioinformatic Analysis on Caffeine Synthase in Plants[J]. Fujian Journal of Agricultural Sciences, 2014, 29(12): 1211-1218. DOI: 10.19303/j.issn.1008-0384.2014.12.011
[8]	LI You-juan, GE Jun-qing, LIN Tian-long. Cloning and Bioinformatics Analysis of ORF51 Gene of Anguillae Herpesvirus FJ Strain[J]. Fujian Journal of Agricultural Sciences, 2014, 29(4): 314-318. DOI: 10.19303/j.issn.1008-0384.2014.04.004
[9]	ZHANG Zhi-deng, LUO Tu-yan, RAO Qiu-hua, CAO Zhi-yun, ZHENG Teng. Glutamine Synthetase Gene Amplification by 3'RACE and Bioinformatics Analysis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 919-923.
[10]	LIU Shu-ji, WU Cheng-ye, SHAN Chao, WANG Ai-rong, WANG Zong-hua. Bioinformatic analysis of angiotensin converting enzyme in Danio rerio[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 205-211.

Cited By

Get Citation

PDF

XML

Article Metrics

Article views (915) PDF downloads (84)

Cloning and Bioinformatics of Chalcone Synthase Gene of Moringa oleifera

Abstract

References

Related Articles

Catalog

Article Metrics

Related

Cloning and Bioinformatics of Chalcone Synthase Gene of Moringa oleifera

Abstract

References

Related Articles

Catalog

Article Metrics

Related

Export File

Citation

Format

Content