Expression and Distribution of Nucleic Acid-sensing TLRs in Porcine Sperm

ZHANG Linli; LI Li; XIN Qingwu; ZHU Zhiming; MIAO Zhongwei; LIN Changguang; LI Zhaolong; ZHENG Nenzhu

doi:10.19303/j.issn.1008-0384.2021.04.009

Volume 36 Issue 4

Apr. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(4): 440-444

ZHANG L L, LI L, XIN Q W, et al. Expression and Distribution of Nucleic Acid-sensing TLRs in Porcine Sperm [J]. Fujian Journal of Agricultural Sciences，2021，36（4）：440−444 doi: 10.19303/j.issn.1008-0384.2021.04.009

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ZHANG L L, LI L, XIN Q W, et al. Expression and Distribution of Nucleic Acid-sensing TLRs in Porcine Sperm [J]. Fujian Journal of Agricultural Sciences，2021，36（4）：440−444 doi: 10.19303/j.issn.1008-0384.2021.04.009

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Expression and Distribution of Nucleic Acid-sensing TLRs in Porcine Sperm

doi: 10.19303/j.issn.1008-0384.2021.04.009

1.
Institute of Animal Science and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Fujian Guanghua Best Eco-agriculture Development Co., Ltd., Fuzhou, Fujian　350003, China

Received Date: 2020-11-30
Rev Recd Date: 2021-03-03

Available Online: 2021-04-20

Publish Date: 2021-04-30

Abstract

Abstract

Objective Expression and distribution of the toll-like receptor (TLR) in porcine sperm were analyzed to facilitate the study on the related signaling pathway and antimicrobial properties. Method The mRNA expressions of TLR3, 7, 8, and 9 in pig sperm were detected by RT-PCR, and the distribution of TLR3 by immunofluorescence. Result The mRNA of TLR3, 7, 8, and 9 were all expressed in the sperm, and TLR3 mainly found in the posterior head and midpiece. Conclusion The expression of nucleic acid-sensing TLRs and the localizations of TLR3 in the porcine sperm revealed in this study gave clues of how the sperm might respond to attacks by virus and derivatives. The information paved the way for further investigation on the sperm TLR signaling pathway and pathogen recognition function.
- Nucleic acid-sensing TLRs,
- porcine sperm,
- mRNA expression,
- immunofluorescence

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References(21)

References

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