Genomic DNA Extraction for Authentication of <i>Clematis florida</i> Thunb. var. plena D. Don

HE Shulan; Li Yongning; ZHU Furong

doi:10.19303/j.issn.1008-0384.2021.03.003

Volume 36 Issue 3

Mar. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(3): 264-270

HE S L, Li Y N, ZHU F R. Genomic DNA Extraction for Authentication of Clematis florida Thunb. var. plena D. Don [J]. Fujian Journal of Agricultural Sciences，2021，36（3）：264−270 doi: 10.19303/j.issn.1008-0384.2021.03.003

Citation:

HE S L, Li Y N, ZHU F R. Genomic DNA Extraction for Authentication of Clematis florida Thunb. var. plena D. Don [J]. Fujian Journal of Agricultural Sciences，2021，36（3）：264−270 doi: 10.19303/j.issn.1008-0384.2021.03.003

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Genomic DNA Extraction for Authentication of Clematis florida Thunb. var. plena D. Don

doi: 10.19303/j.issn.1008-0384.2021.03.003

Fujian Health College, Fuzhou, Fujian 　350101, China

Received Date: 2020-07-25
Rev Recd Date: 2021-02-03

Available Online: 2021-03-27

Publish Date: 2021-03-31

Abstract

Abstract

Objective A rapid and economical method to extract the genomic DNA from Clematis florida Thunb.var. plena D. Don for commercial authentication of the valuable Chinese herbal medicine was established. Method CTAB, Testing Kit, SDS, and the high salt/low pH methods, along with a modified CTAB method, were applied to extract genomic DNA from leaves of the herbal plant. The quality and integrity of the extracted DNA samples were compared using agarose gel electrophoresis. Then, by bidirectional sequencing, the selected molecular pharmacognosy method was used to differentiate between the authentic material and a counterfeit for methodology verification. Result The modified CTAB method yielded high purity DNA with clear and bright bands on SSR primer and ITS2 sequence amplifications. The PCR amplified products of the extracted DNA were used in the bidirectional sequencing to correctly identify and accurately differentiate the true and faux products. Conclusion The improved CTAB method established in this study could effectively extract the genomic DNA of the target plant material. It was simple, rapid and reliable in extracting high quality DNA for molecular pharmacognosy identification on Clematis florida Thunb. var. plena D. Don.
- Clematis florida Thunb. var. plena D. Don,
- genome,
- DNA extraction,
- identification

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References(23)

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