Expressions of Panicle Development- and Cell Cycle-related Genes in Rice with Clustered Spikelets

CHEN Liping; JIANG Jiahuan; ZHENG Yanmei; ZHU Yongsheng; WANG Yingheng; LIN Qiang; XIE Hongguang; LUO Xi; CAI Qiuhua; XIE Huaan; ZHANG Jianfu

doi:10.19303/j.issn.1008-0384.2021.03.001

Volume 36 Issue 3

Mar. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(3): 249-254

CHEN L P, JIANG J H, ZHENG Y M, et al. Expressions of Panicle Development- and Cell Cycle-related Genes in Rice with Clustered Spikelets [J]. Fujian Journal of Agricultural Sciences，2021，36（3）：249−254 doi: 10.19303/j.issn.1008-0384.2021.03.001

Citation:

CHEN L P, JIANG J H, ZHENG Y M, et al. Expressions of Panicle Development- and Cell Cycle-related Genes in Rice with Clustered Spikelets [J]. Fujian Journal of Agricultural Sciences，2021，36（3）：249−254 doi: 10.19303/j.issn.1008-0384.2021.03.001

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Expressions of Panicle Development- and Cell Cycle-related Genes in Rice with Clustered Spikelets

doi: 10.19303/j.issn.1008-0384.2021.03.001

CHEN Liping^{1, 2, 3
,},
JIANG Jiahuan^{1, 2, 3},
ZHENG Yanmei^{1, 2, 3},
ZHU Yongsheng^{1, 2, 3},
WANG Yingheng^{1, 2, 3},
LIN Qiang^{1, 2, 3},
XIE Hongguang^{1, 2, 3},
LUO Xi^{1, 2, 3},
CAI Qiuhua^{1, 2, 3},
XIE Huaan^{1, 2, 3
,
,},
ZHANG Jianfu^{1, 2, 3
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fujian Academy of Agricultural Sciences/Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, China/South China Bases of National Key Laboratory of Hybrid Rice for China, Fuzhou, Fujian　350003, China
3.
National Rice Engineering Laboratory of China, Fuzhou, Fujian　350003, China

Received Date: 2021-01-13
Rev Recd Date: 2021-03-05

Available Online: 2021-03-27

Publish Date: 2021-03-31

Abstract

Abstract

Objectives Expressions of the genes related to panicle development and cell cycle in the mutant rice with clustered spikelets were studied to pave the road for research with an attempt to increase number of grains per panicle on rice plant for improved yield. Methods The expressions of OsCl-6 and other panicle development-related genes as well as those associated with cell cycle in Neijiang P164 of the mutant variety that bears clustered spikelets on panicle were compared with those in the regular single-floret variety, 9311. Young panicles on Neijiang P164 and 9311 plants that were grown from the seeds in a netted greenhouse were collected for RNA extraction, qRT-PCR, and data analysis. Results The qRT-PCR results showed higher expression of OsCl-6 at different stages of panicle development in Neijiang P164 than 9311. The expressions of cell cycle dependent kinase genes in 9311 was higher than Neijiang P164, while those of class B cyclins in Neijiang P164 higher than 9311. Neijiang P164 had lower expressions on the panicle development-related genes such as LAX1, WOX3, and RFL than did 9311, but higher on MFS1 and CL-4. Conclusions The significant differences in the expressions of the genes related to panicle development and cell cycle, including OsCl-6 and B-type cyclins, between Naijiang P164 and 9311 revealed a potential correlation between the genetic makeup and physiology of the two rice varieties. The upregulated OsCl-6 might inhibit the development of secondary branches on rice plants. As increased grains per panicle would directly boost the yield of a rice plant, clustered spikelets would mean improved crop yield.
- rice,
- clustered spikelets,
- panicle development,
- gene expression

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References(23)

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