Cloning and Sequencing Sucrose Synthase Gene of Alpine Potato

HONG Senrong; DENG Yuqing; WU Hongting; CHEN Ting; GUO Shuzhen; YANG Yuxuan; CAI Hong; CHEN Ronghua

doi:10.19303/j.issn.1008-0384.2021.02.003

Volume 36 Issue 2

Feb. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(2): 148-156

HONG S R, DENG Y Q, WU H T, et al. Cloning and Sequencing Sucrose Synthase Gene of Alpine Potato [J]. Fujian Journal of Agricultural Sciences，2021，36（2）：148−156 doi: 10.19303/j.issn.1008-0384.2021.02.003

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HONG S R, DENG Y Q, WU H T, et al. Cloning and Sequencing Sucrose Synthase Gene of Alpine Potato [J]. Fujian Journal of Agricultural Sciences，2021，36（2）：148−156 doi: 10.19303/j.issn.1008-0384.2021.02.003

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Cloning and Sequencing Sucrose Synthase Gene of Alpine Potato

doi: 10.19303/j.issn.1008-0384.2021.02.003

1.
College of Life Sciences, Shangrao Normal University, Shangrao, Jiangxi　334001, China
2.
Key Laboratory of protection and utilization of medicinal and edible plant resources in Shangrao City, Shangrao, Jiangxi　334001, China
3.
Shangrao Key Laboratory of germplasm conservation and utilization of potato and taro crops, Shangrao, Jiangxi　334001, China
4.
Shangrao Agricultural Technology Innovation Research Institute, Shangrao, Jiangxi　334001, China
5.
Red sun Agricultural Development Co., Ltd., Shangrao, Jiangxi　334700, China

Received Date: 2020-04-09
Rev Recd Date: 2020-06-25

Available Online: 2021-02-08

Publish Date: 2021-02-28

Abstract

Abstract

Objective Sequence of the gene associated with the key enzyme in sucrose metabolism pathway of potatoes, sucrose synthase (SuSy), was determined for studying the biological function of the enzyme. Method The core fragment (PGSC0003DMG400002895, SuSy 4) of SuSy was screened from the transcriptome database on the plantlets of Alpine potatoes (Solanum tuberosum L. cv. Huaiyushan). The gene was cloned by RT-PCR, and its sequence analyzed using the bioinformatics method. Result The total length of cDNA of SuSy gene was 2 418 bp containing 45.08% of G+C. The hydrophilic protein had 805 amino acids with a molecular weight of 92 471.33 Da and an isoelectric point of 5.87. Its secondary structure was 45.84% of alpha helix, 15.16% of β -extended strands, and 39.01% of random coils. The β-lamellae and α-helix were found throughout the entire protein but appeared as irregular, curl, extension chains at the C- and N-terminals. The tetrameric gene mainly existed in the cytoplasm, mitochondria, and chloroplast. It closely related to those of S. lycopersicum, S. pennellii, S. chilense, S. tuberosum, Capsicum annuum, and C. baccatum in evolution, especially, S. tuberosum. Conclusion The SuSy gene in the Alpine potato of Huaiyushan had the characteristic structure of a typical sucrose synthase. The amino acid and nucleic acid sequences were highly conserved and homologous to the similar species. The findings would be of great significance for further understanding on the biological function of the enzyme.
- Alpine potato of Huaiyushan,
- plantlet,
- sucrose synthase,
- cloning,
- sequence analysis

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References(31)

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