Volatiles, Flavonoids, and Antibacterial Activity of Ethanol-extract from <i>Geranium wilfordii</i>

LI Yu; LIU Haihua; LIU Cuijun; YOU Jinwen; ZHAO Renjun; SHI Aihua; HE Meijun

doi:10.19303/j.issn.1008-0384.2020.12.014

Volume 35 Issue 12

Dec. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(12): 1397-1404

LI Y, LIU H H, LIU C J, et al. Volatiles, Flavonoids, and Antibacterial Activity of Ethanol-extract from Geranium wilfordii [J]. Fujian Journal of Agricultural Sciences，2020，35（12）：1397−1404 doi: 10.19303/j.issn.1008-0384.2020.12.014

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LI Y, LIU H H, LIU C J, et al. Volatiles, Flavonoids, and Antibacterial Activity of Ethanol-extract from Geranium wilfordii [J]. Fujian Journal of Agricultural Sciences，2020，35（12）：1397−1404 doi: 10.19303/j.issn.1008-0384.2020.12.014

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Volatiles, Flavonoids, and Antibacterial Activity of Ethanol-extract from Geranium wilfordii

doi: 10.19303/j.issn.1008-0384.2020.12.014

1.
institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi, Hubei　445000, China
2.
Enshi Polytechnic, Enshi, Hubei　445000, China

Received Date: 2020-03-06
Rev Recd Date: 2020-08-28
Publish Date: 2020-12-31

Abstract

Abstract

Objective Antibacterial activity and mechanism of the ethanol-extract of Geranium wilfordii Maxim on clinical pathogens were studied for development of a natural antibiotic. Method The chemical compositions of the extract were analyzed by GC-MS and HPC. The filter paper method was used to test the antimicrobial activity of the extract against 12 clinical samples of the resistant bacteria. Result GC-MS detected 18 volatile substances, including aliphatic hydrocarbons, fatty acids, and alkaloids (1.15%). The 3 flavonoid compounds identified were myricitrin, quercetin-3-O-β-D-glucose, and taxifolin. High antibacterial activities against Enterococcus faecalis ATCC 29212 (MIC 1.0 mg·mL⁻¹), methicillin-resistant Staphylococcus aureus (MIC 1.0 mg·mL⁻¹), Escherichia coli ATCC 25922 (MIC 1.0 mg·mL⁻¹), and Acinetobacter baumannii ATCC 29212 (MIC 2.0 mg·mL⁻¹) of the extract were observed. Conclusion In the G. wilfordii ethanol-extract, aliphatic hydrocarbons, fatty acids, and alkaloids were the major volatiles, and myricitrin, quercetin-3-O-β-D-glucose, and taxifolin the 3 substantial flavonoid compounds found. The extract demonstrated a significant antibacterial activity with wide spectrum against the commonly known clinical pathogens.
- Geranium wilfordii Maxim,
- ethanol-extract,
- volatile substances,
- clinical resistant bacteria,
- flavonoids

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References(29)

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