Microbial Diversity in Rhizosphere and Non-rhizosphere Soils of <i>Paris polyphylla </i>var<i>. chinensis</i> Plants

ZHENG Meixia; CHEN Hong; ZHU Yujing; SU Hailan

doi:10.19303/j.issn.1008-0384.2020.12.009

Volume 35 Issue 12

Dec. 2020

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(12): 1357-1367

ZHENG M X, CHEN H, ZHU Y J, et al. Microbial Diversity in Rhizosphere and Non-rhizosphere Soils of Paris polyphylla var . chinensis Plants [J]. Fujian Journal of Agricultural Sciences，2020，35（12）：1357−1367 doi: 10.19303/j.issn.1008-0384.2020.12.009

Citation:

ZHENG M X, CHEN H, ZHU Y J, et al. Microbial Diversity in Rhizosphere and Non-rhizosphere Soils of Paris polyphylla var . chinensis Plants [J]. Fujian Journal of Agricultural Sciences，2020，35（12）：1357−1367 doi: 10.19303/j.issn.1008-0384.2020.12.009

Citation:

PDF( 1407 KB)

Microbial Diversity in Rhizosphere and Non-rhizosphere Soils of Paris polyphylla var. chinensis Plants

doi: 10.19303/j.issn.1008-0384.2020.12.009

Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China

Received Date: 2020-07-29
Rev Recd Date: 2020-11-11

Available Online: 2020-11-24

Publish Date: 2020-12-31

Abstract

Abstract

Objective The microbial communities in rhizosphere and non-rhizosphere soils growing Paris polyphylla var. chinensis plants were analyzed and compared in the field as well as in laboratory. Method The high throughput sequencing Illumina Miseq 2×300 bp was applied to sequence total DNA of the microbes in the soils. The LDA Effect Size analysis was used to examine the differences on selected microbial groups between the communities in the field and laboratory. Result The microbial diversity in the soils of P. polyphylla was rich with 21 phyla, 33 classes, 52 orders, 89 families, and 160 genera identified. The Chao, Ace, Shannon, and Simpson indices on the soil samples indicated that the non-rhizosphere soil was more abundant and diverse than the rhizosphere soil. However, there were no significant differences between them on the phylum or family level. Among the differentially abundant groups, Chlorobia on the class level, Chlorobiales and Myxococcales on the order level, and Acinetobacter and Rudaea on the genus level were significantly lower in the rhizosphere. Conclusion This study not only shows that Paris polyphylla var. chinensis non-rhizosphere soil bacterial communities have higher abundance and diversity, but also lays the foundation for further exploration of the soil microbial environment of Paris polyphylla var. chinensis.
- Paris polyphylla var. chinensis,
- rhizosphere,
- soil,
- diversity

FullText(HTML)

References(20)

References

[1]	WANG X, PENG C, LIANG J, et al. The complete chloroplast genome of Paris polyphylla var. chinensis, an endemic medicinal herb in China [J]. Mitochondrial DNA Part B, 2019, 4(2): 3888−3889. doi: 10.1080/23802359.2019.1687351
[2]	YANG Z Y, YANG L F, LIU C K, et al. Transcriptome analyses of Paris Polyphylla var. Chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway [J]. Fitoterapia, 2019, 135: 52−63. doi: 10.1016/j.fitote.2019.04.008
[3]	陈倩倩, 刘波, 刘国红, 等. 华重楼根际土芽胞杆菌多样性研究 [J]. 热带农业科学, 2015, 35(12):103−107, 112. doi: 10.3969/j.issn.1009-2196.2015.12.020 CHEN Q Q, LIU B, LIU G H, et al. Diversity of culturable Bacillus species from Paris Linnaeus rhizosphere soil [J]. Chinese Journal of Tropical Agriculture, 2015, 35(12): 103−107, 112.(in Chinese) doi: 10.3969/j.issn.1009-2196.2015.12.020
[4]	张千和, 周立香, 郭荻. 中药材根际和非根际土壤酶和微生物特征 [J]. 西北农业学报, 2014, 23(12):189−196. doi: 10.7606/j.issn.1004-1389.2014.12.029 ZHANG Q H, ZHOU L X, GUO D. Research on soil enzymes and microflora in rhizosphere and non-rhizosphere of traditional Chinese medicinal herbs [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2014, 23(12): 189−196.(in Chinese) doi: 10.7606/j.issn.1004-1389.2014.12.029
[5]	曹永昌, 杨瑞, 刘帅, 等. 秦岭典型林分夏秋两季根际与非根际土壤微生物群落结构 [J]. 生态学报, 2017, 37(5):1667−1676. CAO Y C , YANG R, LIU S, et al. Characteristics of microbial community in forest soil between rhizosphere and non-rhizosphere in summer and autumn in Qinling Mountains, China [J]. Acta Ecologica Sinica, 2017, 37(5): 1667−1676.(in Chinese)
[6]	邱权, 李吉跃, 王军辉, 等. 西宁南山4种灌木根际和非根际土壤微生物、酶活性和养分特征 [J]. 生态学报, 2014, 34(24):7411−7420. QIU Q, LI J Y, WANG J H, et al. Microbes, enzyme activities and nutrient characteristics of rhizosphere and nonrhizosphere soils under four shrubs in Xining Nanshan, Prefecture, China [J]. Acta Ecologica Sinica, 2014, 34(24): 7411−7420.(in Chinese)
[7]	梁娟, 郭泽宇, 叶漪. 不同土壤水分条件对七叶一枝花光合特性及有效成分皂苷含量的影响 [J]. 植物生理学报, 2014, 50(1):56−60. LIANG J, GUO Z Y, YE Y. Effects of different soil moisture conditions on photosynthetic characteristics and effective content of saponin of parispolyphylla [J]. Plant Physiology Communications, 2014, 50(1): 56−60.(in Chinese)
[8]	张静, 肖国生, 周浓, 等. 三峡库区栽培重楼属药用植物根际土壤微生物数量和酶活性的变化 [J]. 中国中医药信息杂志, 2016, 23(10):95−99. doi: 10.3969/j.issn.1005-5304.2016.10.022 ZHANG J, XIAO G S, ZHOU N, et al. Variation of rhizospheric microorganisms and soil enzyme activity of paridis rhizoma cultivated in Three Gorges reservoir region [J]. Chinese Journal of Information on Traditional Chinese Medicine, 2016, 23(10): 95−99.(in Chinese) doi: 10.3969/j.issn.1005-5304.2016.10.022
[9]	欧洪, 郭冬琴, 林俊杰, 等. AM真菌对滇重楼根际土壤微生物数量及酶活性的影响 [J]. 中药材, 2016, 39(5):948−955. OU H, GUO D Q, LIN J J, et al. Effects of different AM fungi on quantity and enzyme activity of rhizosphere soil microorganism of Paris Polyphylla var. Yunnanensis [J]. Jorunal of Chinese Medicinal Materials, 2016, 39(5): 948−955.(in Chinese)
[10]	李金辉, 卢鑫, 周志宇, 等. 不同种植年限紫穗槐根际非根际土壤磷组分含量特征 [J]. 草业学报, 2014, 23(6):61−68. doi: 10.11686/cyxb20140608 LI J H, LU X, ZHOU Z Y, et al. Phosphorus contents in the rhizosphere and bulk soil under Amorpha fruticosa established in different years [J]. Acta Prataculturae Sinica, 2014, 23(6): 61−68.(in Chinese) doi: 10.11686/cyxb20140608
[11]	焦晓丹, 吴凤芝. 土壤微生物多样性研究方法的进展 [J]. 土壤通报, 2004, 35(6):789−792. doi: 10.3321/j.issn:0564-3945.2004.06.026 JIAO X D, WU F Z. Progress of the methods for studying soil microbial diversity [J]. Chinese Journal of Soil Science, 2004, 35(6): 789−792.(in Chinese) doi: 10.3321/j.issn:0564-3945.2004.06.026
[12]	HIRSCH P R, MAUCHLINE T H, CLARK I M. Culture-independent molecular techniques for soil microbial ecology [J]. Soil Biology and Biochemistry, 2010, 42(6): 878−887. doi: 10.1016/j.soilbio.2010.02.019
[13]	刘慧娟, 吴其国, 祁冰洁, 等. 药用植物根际微生物研究现状及前景 [J]. 宜春学院学报, 2019, 41(6):96−101. LIU H J, WU Q G, QI B J, et al. Research Status and Prospect on Rhizosphere Microbiome of Medicinal Plants [J]. Journal of Yichun University, 2019, 41(6): 96−101.(in Chinese)
[14]	卜洪震, 王丽宏, 肖小平, 等. 双季稻区稻田不同土壤类型的微生物群落多样性分析 [J]. 作物学报, 2010, 36(5):826−832. doi: 10.3724/SP.J.1006.2010.00826 BU H Z, WANG L H, XIAO X P, et al. Diversity of microbial community of paddy soil types in double-rice cropping system [J]. Acta Agronomica Sinica, 2010, 36(5): 826−832.(in Chinese) doi: 10.3724/SP.J.1006.2010.00826
[15]	黄珍, 谭志琼, 阮云泽. 香蕉园土壤16S rDNA文库分析 [J]. 热带作物学报, 2010, 31(6):989−993. doi: 10.3969/j.issn.1000-2561.2010.06.021 HUANG Z, TAN Z Q, RUAN Y Z. Phylogenetic Diversity of Bacteria in Banana Soils Determined with 16S rDNA Library Analysis [J]. Chinese Journal of Tropical Crops, 2010, 31(6): 989−993.(in Chinese) doi: 10.3969/j.issn.1000-2561.2010.06.021
[16]	葛晓颖, 孙志刚, 李涛, 等. 设施番茄连作障碍与土壤芽孢杆菌和假单胞菌及微生物群落的关系分析 [J]. 农业环境科学学报, 2016, 35(3):514−523. doi: 10.11654/jaes.2016.03.015 GE X Y, SUN Z G, LI T, et al. Soil Pseudomonas spp., Bacillus spp., and microbial communities under tomato continuous cropping in greenhouse production [J]. Journal of Agro-Environment Science(J. Agro-Environ. Sci.), 2016, 35(3): 514−523.(in Chinese) doi: 10.11654/jaes.2016.03.015
[17]	马鸣超, 刘丽, 姜昕, 等. 胶质类芽孢杆菌与慢生大豆根瘤菌复合接种效果评价 [J]. 中国农业科学, 2015, 48(18):3600−3611. doi: 10.3864/j.issn.0578-1752.2015.18.004 MA M C, LIU L, JIANG X, et al. Evaluation of the effect of Co-lnoculant of Paenibacillus mucilaginosus and Bradyrhizobium japonicum in application [J]. Scientia Agricultura Sinica, 2015, 48(18): 3600−3611.(in Chinese) doi: 10.3864/j.issn.0578-1752.2015.18.004
[18]	常文智, 马鸣超, 李力, 等. 施用胶质类芽孢杆菌对土壤生物活性和花生产量的影响 [J]. 中国土壤与肥料, 2014(1):84−89. CHANG W Z, MA M C, LI L, et al. Effects of Paenibacillus mucilaginosus on soil biological activity and yield of peanut [J]. Soil and Fertilizer Sciences in China, 2014(1): 84−89.(in Chinese)
[19]	韩丽珍, 刘畅, 周静. 接种促生菌对花生根际土壤微生物及营养元素的影响 [J]. 基因组学与应用生物学, 2019, 38(7):3065−3073. HAN L Z, LIU C, ZHOU J. Effects of Inoculation with Growth-Promoting Bacteria on Peanut Rhizosphere Soil Microorganism and Nutrient Elements [J]. Genomics and Applied Biology, 2019, 38(7): 3065−3073.(in Chinese)
[20]	何志刚, 王秀娟, 董环, 等. 日光温室辣椒连作不同年限土壤微生物种群变化及酶活性研究 [J]. 中国土壤与肥料, 2013(1):38−42. HE Z G, WANG X J, DONG H, et al. Study on microbial and enzyme activity of Capsicum soil different ages in sunlight greenhouse [J]. Soil and Fertilizer Sciences in China, 2013(1): 38−42.(in Chinese)