Effects of Hydrogen Sulfide on Physiology and Photosynthesis of Barley Seedlings under Cd-stress

LI Xiaoke; WU Yuzhen; ZHANG Jinhua; ZHANG Yixian

doi:10.19303/j.issn.1008-0384.2020.10.011

Volume 35 Issue 10

Oct. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(10): 1131-1137

LI X K, WU Y Z, ZHANG J H, et al. Effects of Hydrogen Sulfide on Physiology and Photosynthesis of Barley Seedlings under Cd-stress [J]. Fujian Journal of Agricultural Sciences，2020，35（10）：1131−1137 doi: 10.19303/j.issn.1008-0384.2020.10.011

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LI X K, WU Y Z, ZHANG J H, et al. Effects of Hydrogen Sulfide on Physiology and Photosynthesis of Barley Seedlings under Cd-stress [J]. Fujian Journal of Agricultural Sciences，2020，35（10）：1131−1137 doi: 10.19303/j.issn.1008-0384.2020.10.011

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Effects of Hydrogen Sulfide on Physiology and Photosynthesis of Barley Seedlings under Cd-stress

doi: 10.19303/j.issn.1008-0384.2020.10.011

1.
School of Biology Science and Technology, Jinzhong College, Jinzhong, Shanxi　030619, China
2.
School of Life Science, Shanxi University, Taiyuan, Shanxi　030006, China

Received Date: 2020-02-18
Rev Recd Date: 2020-08-03
Publish Date: 2020-10-28

Abstract

Abstract

Objective Rule of H₂S played on the growth of barley seedlings under Cd-stress was investigated to understand the mechanism and means to improve Cd-tolerance of the plants. Method Treatment groups of Cd, NaHS, HA, Cd+NaHS, and Cd+HA with addition of CdCl₂ at 0.2 mmol·L⁻¹, exogenous H₂S donor NaHS at 50 μmol·L⁻¹ or H₂S synthesis inhibitor hydroxylamine HA at 1 mmol·L⁻¹ in medium were made for an indoor hydroponic barley seedling growth experiment. Effects of the treatments on the growth, leaf damage, osmotic regulator, antioxidant enzyme activity, chlorophyll content, and photosynthetic characteristics of the seedlings of Jinke 571 barley were monitored. Result The H₂S addition in the hydroponic medium lessened the growth inhibition and leaf damage on the seedlings induced by Cd-stress. It increased the root length, plant height, and seedling biomass; decreased the leaf relative conductivity, malondialdehyde (MDA) and superoxide anion (O₂⁻) contents; enhanced the photosynthesis with increased chlorophyll content, net photosynthetic rate, stomatal conductance, and transpiration rate; and, improved the resistance to the external adversity imposed on them with increased osmotic regulatory substances (e.g., soluble sugar, proline, soluble protein) and antioxidant enzymatic activities on SOD, POD, CAT. Conclusion Addition of H₂S donor NaHS at 50 μmol·L⁻¹ in hydroponic medium partially alleviated the ill-effects of Cd-stress on the barley seedlings suggesting its potential field application for barley farming to combat the pollution.
- Cd-stress,
- exogenous H₂S,
- Hordeum vulgare,
- physiological indices

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References(27)

References

[1]	叶仙勇, 沈磊. 我国土壤环境污染的现状分析与防治措施 [J]. 资源节约与环保, 2016, 38(4):178−180. doi: 10.3969/j.issn.1673-2251.2016.04.150 YE X Y, SHEN L. Analysis of the present situation of soil environmental pollution in China and its prevention and control measures [J]. Resources Economization & Environment Protection, 2016, 38(4): 178−180.(in Chinese) doi: 10.3969/j.issn.1673-2251.2016.04.150
[2]	何俊瑜, 任艳芳, 王阳阳, 等. 不同耐性水稻幼苗根系对镉胁迫的形态及生理响应 [J]. 生态学报, 2011, 31(2):522−528. HE J Y, REN Y F, WANG Y Y, et al. Root morphological and physiological responses of rice seedlings with different tolerance to cadmium stress [J]. Acta Ecologica Sinica, 2011, 31(2): 522−528.(in Chinese)
[3]	闫晶, 姬文秀, 石贤吉, 等. 镉胁迫对烟草种子萌发和烟苗生长发育的影响 [J]. 作物学报, 2019, 45(2):142−149. YAN J, JI W X, SHI X J, et al. Effects of cadmium stress on seed germination and seedling growth of tobacco [J]. Acta Agronomica Sinica, 2019, 45(2): 142−149.(in Chinese)
[4]	田雲, 蒋景龙, 李丽, 等. 信号分子硫化氢调控植物抗逆性研究进展 [J]. 核农学报, 2017, 31(11):2279−2281. doi: 10.11869/j.issn.100-8551.2017.11.2279 TIAN Y, JIANG J L, LI L, et al. Research advances in plant stress resistance regulated by signal molecule hydrogen sulfide [J]. Journal of Nuclear Agricultural Sciences, 2017, 31(11): 2279−2281.(in Chinese) doi: 10.11869/j.issn.100-8551.2017.11.2279
[5]	LI Z G, MIN X, ZHOU Z H. Hydrogen Sulfide: A signal molecule in plant cross-adaptation [J]. Frontiers in Plant Science, 2016, 7(42): 1621.
[6]	张丽萍, 刘志强, 金竹萍, 等. H₂S对镉胁迫下白菜幼苗根系渗透胁迫的调节作用 [J]. 农业环境科学学报, 2016, 35(2):247−252. doi: 10.11654/jaes.2016.02.006 ZHANG L P, LIU Z Q, JIN Z P, et al. Regulation of H₂S on Cd-induced osmotic stress in roots of Chinese cabbage seedling [J]. Journal of Agro-Environment Science, 2016, 35(2): 247−252.(in Chinese) doi: 10.11654/jaes.2016.02.006
[7]	于立旭, 尚宏芹, 张存家, 等. 外源硫化氢对镉胁迫下黄瓜胚轴和胚根生理生化特性的影响 [J]. 园艺学报, 2011, 38(11):2131−2139. YU L X, SHANG H Q, ZHANG C J, et al. Effects of exogenous H₂S on the physiological and biochemical characteristics of the cucumber hypocotyls and radicles under cadmium stress [J]. Acta Horticulturae Sinica, 2011, 38(11): 2131−2139.(in Chinese)
[8]	贾红磊, 刘珂娜, 刘洋, 等. 硫化氢在缓解拟南芥镉胁迫中的作用 [J]. 陕西科技大学学报, 2018, 36(5):23−27. doi: 10.3969/j.issn.1000-5811.2018.05.004 JIA H L, LIU K N, LIU Y, et al. Effect of hydrogen sulfide on reducing cadmium stress in Arabidopsis thaliana [J]. Journal of Shanxi University of Science & Technology, 2018, 36(5): 23−27.(in Chinese) doi: 10.3969/j.issn.1000-5811.2018.05.004
[9]	田保华. 硫化氢信号在谷子响应镉胁迫中的生理作用[D]. 太原: 山西大学, 2016. TIAN B H. Physiological effects of hydrogen sulfide signal on response of millet to cadmium stress [D]. Taiyuan: Shanxi University, 2016. (in Chinese).
[10]	张义贤, 李晓科. 镉、铅及其复合污染对大麦幼苗部分生理指标的影响 [J]. 植物研究, 2008, 28(1):43−46. doi: 10.7525/j.issn.1673-5102.2008.01.010 ZHANG Y X, LI X K. Effects of Cd, Pb and their combined pollution on physiological indexes in leaf of the Hordeum vulgare seedling [J]. Bulletin of Botanical Research, 2008, 28(1): 43−46.(in Chinese) doi: 10.7525/j.issn.1673-5102.2008.01.010
[11]	肖志华, 张义贤, 张喜文, 等. 外源铅、铜胁迫对不同基因型谷子幼苗生理生态特性的影响 [J]. 生态学报, 2012, 32(3):889−897. doi: 10.5846/stxb201108281256 XIAO Z H, ZHANG Y X, ZHANG X W, et al. Effects of exogenous Pb and Cu stress on eco-physiological characteristics on foxtail millet seedlings of different genotypes [J]. Acta Ecologica Sinica, 2012, 32(3): 889−897.(in Chinese) doi: 10.5846/stxb201108281256
[12]	李合生. 植物生理生化实验原理和技术 [M]. 北京: 高等教育出版社, 2000: 184-185, 258-261.
[13]	XIE Z, DUAN L, TIAN X, et al. Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radical-scavenging activity [J]. Journal of Plant Physiology, 2008, 165(4): 375−384. doi: 10.1016/j.jplph.2007.06.001
[14]	王晓娟, 王文斌, 杨龙吴, 等. 重金属镉(Cd)在植物体内的转运途径及其调控机制 [J]. 生态学报, 2015, 35(23):7921−7929. WANG X J, WANG W B, YANG L W, et al. Transport pathways of cadmium(Cd) and its regulatory mechanisms in plant [J]. Acta Ecologica Sinica, 2015, 35(23): 7921−7929.(in Chinese)
[15]	ZHANG H, HAO J, JIANG C X, et al. Hdrogen sulfide protects soybean seedlings against drought-induced oxidative stress [J]. Acta Physiologia Plantarum, 2010(32): 849−857.
[16]	MOSTOFA M G, RAHMAN A, ANSARY M M, et al. Hydrogen sulfide modulates cadmium-induced physiolo -gical and biochemical responses to alleviate cadmium toxicity in rice [J]. Sci Rep., 2014, 5: 14078.
[17]	ZHANG H, HU L Y, HU K D, et al. Hydrogen sulfide promotes wheat seed germination and alleviates oxidative damage against copper stress [J]. Journal of Integrative Plant Biology, 2008, 50(12): 1518−1529. doi: 10.1111/j.1744-7909.2008.00769.x
[18]	DAI H F, XU Y J, ZHAO L F, et al. Alleviation of copper toxicity on chloroplast antioxidant capacity and photosystem II photochemistry of wheat by hydrogen sulfide [J]. Brazilian Journal of Botany, 2016, 39(2): 787−793. doi: 10.1007/s40415-016-0250-6
[19]	金竹萍, 裴雁曦. 植物H₂S气体信号分子的生理功能研究进展 [J]. 中国细胞生物学学报, 2013, 35(6):880−888. doi: 10.11844/CJCB.2013.06.0056 JIN Z P, PEI Y X. Research Progress on Hydrogen Sulfide Signaling in Plants [J]. Chinese journal of cell biology, 2013, 35(6): 880−888.(in Chinese) doi: 10.11844/CJCB.2013.06.0056
[20]	张义贤, 付亚萍, 肖志华, 等. 铅胁迫对不同基因型谷子幼苗生理特性及基因组DNA多态性的影响 [J]. 农业环境科学学报, 2013, 32(3):478−484. ZHANG Y X, FU Y P, XIAO Z H, et al. Effects of Pb²⁺ stress on physiological characteristics and DNA polymorphism of genome in different genotypes foxtail millet [J]. Journal of Agro-Environment Science, 2013, 32(3): 478−484.(in Chinese)
[21]	ZHANG H, YU L Y, LI P, et al. Hydrogen sulfide alleviated chromium toxicity in wheat [J]. Biologia Plantarum, 2014, 54(4): 743−747.
[22]	李永生, 方永丰, 李玥, 等. 外源硫化氢对PEG模拟干旱胁迫下玉米种子萌发及幼苗生长的影响 [J]. 核农学报, 2016, 30(4):813−821. doi: 10.11869/j.issn.100-8551.2016.04.0813 LI Y S, FANG Y F, LI Y, et al. Effects of Exogenous Hydrogen Sulfide on Seed Germination and Seedling Growth Under PEG Stimulated Drought Stress in Maize [J]. Journal of Nuclear Agricultural Sciences, 2016, 30(4): 813−821.(in Chinese) doi: 10.11869/j.issn.100-8551.2016.04.0813
[23]	张杰, 马晓寒, 陈彪. 外源硫化氢对干旱胁迫下烟草幼苗生理生化特性的影响 [J]. 中国农业科技导报, 2018, 20(11):112−119. ZHANG J, MA X H, CHEN B. Effects of exogenous hydrogen sulfide on physiological and biochemical characteristics of tobacco seedlings under drought stress [J]. Journal of Agricultural Science and Technology, 2018, 20(11): 112−119.(in Chinese)
[24]	郑爱珍. 镉胁迫对芥蓝根系质膜过氧化及ATPase活性的影响 [J]. 生态学报, 2012, 32(2):483−488. doi: 10.5846/stxb201012131770 ZHEN A Z. Effects of cadmium on lipid peroxidation and ATPase activity of plasma membrane from Chinese kale (Brassica alboglabra Bailey) roots [J]. Acta Ecologica Sinica, 2012, 32(2): 483−488.(in Chinese) doi: 10.5846/stxb201012131770
[25]	郑州元, 林海荣, 崔辉梅. 外源硫化氢对盐胁迫下加工番茄幼苗光合参数及叶绿素荧光特性的影响 [J]. 核农学报, 2017, 31(7):1426−1435. doi: 10.11869/j.issn.100-8551.2017.07.1426 ZHEN Z Y, LIN H R, CUI H M. Effect of exogenous hydrogen sulfide on photosynthesis parameters and chlorophyll fluorescence characteristics of processing tomato [J]. Journal of Nuclear Agricultural Sciences, 2017, 31(7): 1426−1435.(in Chinese) doi: 10.11869/j.issn.100-8551.2017.07.1426
[26]	CHEN Z, CHEN M, JIANG M. Hydrogen sulfide alleviates mercury toxicity by sequestering it in roots or regulating reactive oxygen species productions in rice seedlings [J]. Plant Physiology and Biochemistry, 2017, 111: 179−192. doi: 10.1016/j.plaphy.2016.11.027
[27]	ALI B, GILL R A, YANG S, et al. Hydrogen sulfide alleviates cadmium-induced morpho-physiological and ultrastructural changes in Brassica napus [J]. Ecotoxicology & Environmental Safety, 2014, 110(11): 197−207.