Diversity of Ammonia-oxidizing Microorganisms in Soils of Different Land Use on Wuyi Mountains

LI Wei; HUANG Biao; HUANG Minmin; LIU Wenjing; GUO Jia; LIN Xiangxin

doi:10.19303/j.issn.1008-0384.2020.10.010

Volume 35 Issue 10

Oct. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(10): 1119-1130

LI W, HUANG B, HUANG M M, et al. Diversity of Ammonia-oxidizing Microorganisms in Soils of Different Land Use on Wuyi Mountains [J]. Fujian Journal of Agricultural Sciences，2020，35（10）：1119−1130 doi: 10.19303/j.issn.1008-0384.2020.10.010

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LI W, HUANG B, HUANG M M, et al. Diversity of Ammonia-oxidizing Microorganisms in Soils of Different Land Use on Wuyi Mountains [J]. Fujian Journal of Agricultural Sciences，2020，35（10）：1119−1130 doi: 10.19303/j.issn.1008-0384.2020.10.010

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Diversity of Ammonia-oxidizing Microorganisms in Soils of Different Land Use on Wuyi Mountains

doi: 10.19303/j.issn.1008-0384.2020.10.010

Research Institute of Agricultural Quality Standards and Testing Technology, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou, Fujian　350003, China

Received Date: 2020-04-20
Rev Recd Date: 2020-07-12
Publish Date: 2020-10-28

Abstract

Abstract

Objective Characteristics and seasonal changes of ammonia-oxidizing microorganism (AOM) community in soils under different land uses on Wuyi Mountains were studied. Method Soil specimens of virgin woodland and tea plantations in evergreen broad-leaf forest growing zones at same latitude on Wuyi Mountains in Fujian were collected for Illumina Hiseq high-throughput sequencing to analyze the relative abundance and diversity of AOMs in the region. The data were applied for a correlation analysis with the physiochemical properties of the local soil. Result (1) An abundant resource of AOMs was found in the soils. The microbial species in soil were similar despite varied land uses. The archaea domain of the AOMs included Unclassified Candidatus Nitrososphaera, Unclassified Crenarchaeota, Unclassified Thaumarchaeota, Nitrosopumilus, and others, while the bacteria consisted of Nitrosomonas, Nitrosospira, Unclassified Nitrosomonadaceae and others. At the tea plantations, a unique species, Nitrosovibrio, was found. (2) The relative abundance of AOMs in the region had significantly changed over time and was affected by the seasons. (3) The environmental factors affected the bacteria and the archaea of the AOM community in soil differently. The major influential factors also varied in accordance with seasons; and, potassium in soil affected mainly the bacteria, while ammonium nitrogen the archaea. Conclusion Regular seasonal changes on the AOM community in the soil of evergreen broad-leaf forest zone on Wuyi Mountains were observed. Although the microbial species in the soils did not differ significantly by the land use, their relative abundance varied significantly seasonally and by the land utilization.
- Illumina Hiseq high-throughput sequencing,
- land use,
- ammonia-oxidizing bacteria,
- ammonia-oxidizing archaea

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