Constructions of Banana <i>MaAQP1</i> Bait Vector and Drought-resistance cDNA Library

XU Yi; LI Yujia; WEI Qing; WANG Anbang; WANG Xiaoyi; SONG Shun; LI Jingyang

doi:10.19303/j.issn.1008-0384.2020.10.005

Volume 35 Issue 10

Oct. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(10): 1078-1085

XU Y, LI Y J, WEI Q, et al. Constructions of Banana MaAQP1 Bait Vector and Drought-resistance cDNA Library [J]. Fujian Journal of Agricultural Sciences，2020，35（10）：1078−1085 doi: 10.19303/j.issn.1008-0384.2020.10.005

Citation:

XU Y, LI Y J, WEI Q, et al. Constructions of Banana MaAQP1 Bait Vector and Drought-resistance cDNA Library [J]. Fujian Journal of Agricultural Sciences，2020，35（10）：1078−1085 doi: 10.19303/j.issn.1008-0384.2020.10.005

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Constructions of Banana MaAQP1 Bait Vector and Drought-resistance cDNA Library

doi: 10.19303/j.issn.1008-0384.2020.10.005

XU Yi^{1, 2
,},
LI Yujia¹,
WEI Qing¹,
WANG Anbang¹,
WANG Xiaoyi¹,
SONG Shun^{1, 2},
LI Jingyang^{1
,
,}

1.
Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Banana Genetic Improvement, Haikou, Hainan　571101, China
2.
Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan　572000, China

Received Date: 2020-06-15
Rev Recd Date: 2020-07-29
Publish Date: 2020-10-28

Abstract

Abstract

Objective To clone MaAQP1 from a drought stressed banana plant using a constructed bait vector, and establish a cDNA library of the transformed drought-resistance gene from single-hybrids yeast cells. Method The promoter of MaAQP1 from a banana plant (Musa acuminat L. AAA group cv. Brazilian) under draught-stress was constructed on the pHIS2 plasmid as the bait to be transformed into yeast cells for the gene cloning. A cDNA library of MaAQP1 in the yeast was subsequently established for the study. Result The MaAQP1 bait vector was successfully constructed. The sequence of the 1 362 bp promoter was cloned and analyzed to show 72 cis-acting TATA-box and CAAT-box core elements as well as elements of MYB, MYC, ERE, and MeJA as well as those of ABA, light, and meristem responses, etc. A drought-resistance cDNA library of 1.25×10⁷ CFU in capacity with an average insert size of about 1 200 bp was established. Conclusion The results provided a basis for screening transcription factors of the cloned MaAQP1 in the yeast single-hybrids to decipher the mechanism of MaAQP1 in response to drought for improving the stress resistance of plants.
- Banana,
- drought,
- MaAQP1,
- bait vector,
- cDNA library

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References(27)

References

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