Effect of Glycine Betaine on Seed Germination and Antioxidant Enzymes in Seedlings of <i>Astragalus membranaceus</i> under Salt Stress

HAN Duohong; WANG Enjun; ZHANG Yong

doi:10.19303/j.issn.1008-0384.2020.08.012

Volume 35 Issue 8

Aug. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(8): 891-895

HAN D H, WANG E J, ZHANG Y. Effect of Glycine Betaine on Seed Germination and Antioxidant Enzymes in Seedlings of Astragalus membranaceus under Salt Stress [J]. Fujian Journal of Agricultural Sciences，2020，35（8）：891−895 doi: 10.19303/j.issn.1008-0384.2020.08.012

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HAN D H, WANG E J, ZHANG Y. Effect of Glycine Betaine on Seed Germination and Antioxidant Enzymes in Seedlings of Astragalus membranaceus under Salt Stress [J]. Fujian Journal of Agricultural Sciences，2020，35（8）：891−895 doi: 10.19303/j.issn.1008-0384.2020.08.012

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Effect of Glycine Betaine on Seed Germination and Antioxidant Enzymes in Seedlings of Astragalus membranaceus under Salt Stress

doi: 10.19303/j.issn.1008-0384.2020.08.012

1.
College of life sciences and engineering, Hexi University, Zhangye, Gansu　734000, China
2.
Key Provincial Laboratory of Universities in Gansu Province for Characteristic Resources Utility in Hexi Corridor, Zhangye, Gansu　734000, China

Received Date: 2019-12-06
Rev Recd Date: 2020-07-03
Publish Date: 2020-08-19

Abstract

Abstract

Objective Effects of exogenous treatment of glycine betaine (GB) on seeds and seedlings of Astragalus membranaceus under salt stress was studied to decipher the stress alleviation. Method The seeds and seedlings of A. membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao (Am H) and A. membranaceus (Fisch.) Bge. (Am B) under 100 mmol·L⁻¹ NaCl stress were treated with GB 0–40 mmol·L⁻¹. The rate, potential, and index of seed germination as well as the seedling vigor index and relative salt damage occurrence percentage to the plants were determined. The activities of SOD, CAT, POD, APX, GR and DHAR in the seedlings were analyzed. Result Addition of GB 30 mmol·L⁻¹ to the salt containing medium increased the germination rate of Am H seeds by 159.07% and of Am B seeds by 167.01%, while reduced the relative rate of salt damage on Am H seedlings by 74.38% and on Am B seedlings by 76.18% in comparison to those without the addition. Under salt stress, the seedlings treated with GB 10–30 mmol·L⁻¹ raised on the SOD, CAT, POD, APX, GR and DHAR activities in varying degrees. In general, the activity increases were greater on seedlings of Am B than those of Am H. Conclusion The addition of GB 30 mmol·L⁻¹ effectively ameliorated the adverse effects of the salt stress on the seeds and seedlings of A. membranaceus and more pronounced on Am B than on Am H.

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References

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