Isolation, Identification, and Characterization of Omethoate-degrading <i>Pseudomonas abietaniphila</i> ZZY-C13-1-9

CHEN Rui; QU Jia; MEN Xin; DENG Yuan; SUN Xiaoyu; ZHAO Lingxia; SHEN Weirong

doi:10.19303/j.issn.1008-0384.2020.08.009

Volume 35 Issue 8

Aug. 2020

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(8): 869-875

CHEN R, QU J, MEN X, et al. Isolation, Identification, and Characterization of Omethoate-degrading Pseudomonas abietaniphila ZZY-C13-1-9 [J]. Fujian Journal of Agricultural Sciences，2020，35（8）：869−875 doi: 10.19303/j.issn.1008-0384.2020.08.009

Citation:

CHEN R, QU J, MEN X, et al. Isolation, Identification, and Characterization of Omethoate-degrading Pseudomonas abietaniphila ZZY-C13-1-9 [J]. Fujian Journal of Agricultural Sciences，2020，35（8）：869−875 doi: 10.19303/j.issn.1008-0384.2020.08.009

Citation:

PDF( 794 KB)

Isolation, Identification, and Characterization of Omethoate-degrading Pseudomonas abietaniphila ZZY-C13-1-9

doi: 10.19303/j.issn.1008-0384.2020.08.009

CHEN Rui^{1, 2
,},
QU Jia^{1, 2},
MEN Xin^{1, 2},
DENG Yuan¹,
SUN Xiaoyu^{1, 2},
ZHAO Lingxia^{1, 2},
SHEN Weirong¹

1.
Microbial Resources Research Center, Shaanxi Provincial Institute of Microbiology, Xi’an, Shaanxi　710043, China
2.
Engineering Center of Mt. Qinling Natural Products, Shaanxi Academy of Sciences, Xi’an, Shaanxi　710043, China

Received Date: 2020-02-12
Rev Recd Date: 2020-04-28
Publish Date: 2020-08-19

Abstract

Abstract

Objective Omethoate-degrading bacteria were isolated from shed soil to determine their capacities in decomposing the pesticide for possible application in the field. Method Single carbon source culture was used to screen microbes for the potential purpose followed by morphological observation and 16S RNA sequencing for identification. The pesticide-degrading ability of the isolates was determined by molybdate blue spectrophotometry and HPLC, then verified by a shaking flask method in the laboratory. The culture broth for the challenge test containing the selected isolates, one at a time, was artificially added with omethoate at various concentrations. Result A strain, ZZY-C13-1-9, could grow normally when the pesticide was at 1 000 mg·L⁻¹. It was identified as Pseudomonas abietaniphila and capable of degrading 92.3% of omethoate at 400 mg·L⁻¹ level in the nutrient broth within 240 h at the condition of 30 ℃ and 180 r·min⁻¹. In addition, the strain was found to tolerant DDVP and Dipterex in high concentration as well. In a laboratory test at 25–34 ℃ on soil with a moisture content of 25%–30% and omethoate at 200 mg·L⁻¹, ZZY-C13-1-9 decomposed 59.2% of the pesticide in 45 d. Conclusion A bacterium capable of effectively degrading omethoate in soil was successfully isolated, identified, and characterized.
- Prokaryotic,
- Pseudomonas abietaniphila,
- omethoate,
- biodegradation

FullText(HTML)

References(33)

References

[1]	SCOY A, PENNELL A, ZHANG X Y. Environmental fate and toxicology of dimethoate [J]. Reviews of Environmental Contamination and Toxicology, 2016, 237: 53−70. doi: 10.1007/978-3-319-23573-8_3
[2]	和文祥, 郑粉莉, 田海霞. 氧化乐果对土壤酶活性的影响 [J]. 中国农业科学, 2009, 42(12):4282−4287. doi: 10.3864/j.issn.0578-1752.2009.12.020 HE W X, ZHENG F L, TIAN H X. Effect of omethoate on soil enzyme activities [J]. Scientia Agricultura Sinica, 2009, 42(12): 4282−4287.(in Chinese) doi: 10.3864/j.issn.0578-1752.2009.12.020
[3]	CALATAYUD-VERNICH P, CALATAYUD F, SIMÓ E, et al. A two-year monitoring of pesticide hazard in-hive: High honey bee mortality rates during insecticide poisoning episodes in apiaries located near agricultural settings [J]. Chemosphere, 2019, 232: 471−480. doi: 10.1016/j.chemosphere.2019.05.170
[4]	宋志慧, 刘冰. 氧化乐果对小球藻的毒性研究 [J]. 生态毒理学报, 2014, 9(3):483−489. SONG Z H, LIU B. Toxic effects of omethoate on Chlorella vulgaris [J]. Asian Journal of Ecotoxicology, 2014, 9(3): 483−489.(in Chinese)
[5]	张元园. 氧化乐果对斑马鱼的毒性作用[D]. 青岛: 青岛科技大学, 2016. ZHANG Y Y. Toxicity effect of omethoate to Danio rerio[D]. Qingdao: Qingdao University of Science & Technology, 2016.(in Chinese)
[6]	LOTTI M, MORETTO A. Organophosphate-induced delayed polyneuropathy [J]. Toxicological Reviews, 2005, 24(1): 37−49. doi: 10.2165/00139709-200524010-00003
[7]	HUO D, JIANG S L, QIN Z, et al. Omethoate induces pharyngeal cancer cell proliferation and G1/S cell cycle progression by activation of Akt/GSK-3β/cyclin D1 signaling pathway [J]. Toxicology, 2019, 427: 152298. doi: 10.1016/j.tox.2019.152298
[8]	COSTA L G. Organophosphorus compounds at 80: Some old and new issues [J]. Toxicological Sciences, 2018, 162(1): 24−35. doi: 10.1093/toxsci/kfx266
[9]	KATSIKANTAMI I, COLOSIO C, ALEGAKIS A, et al. Estimation of daily intake and risk assessment of organophosphorus pesticides based on biomonitoring data-The internal exposure approach [J]. Food and Chemical Toxicology, 2019, 123: 57−71. doi: 10.1016/j.fct.2018.10.047
[10]	SINGH B K, WALKER A. Microbial degradation of organophosphorus compounds [J]. FEMS Microbiology Reviews, 2006, 30(3): 428−471. doi: 10.1111/j.1574-6976.2006.00018.x
[11]	张娜娜, 姜博, 邢奕, 等. 有机磷农药污染土壤的微生物降解研究进展 [J]. 土壤, 2018, 50(4):645−655. ZHANG N N, JIANG B, XING Y, et al. Microbial degradation of organophosphorus pesticide contaminated soils [J]. Soils, 2018, 50(4): 645−655.(in Chinese)
[12]	MATSUMURA F, BOUSH G M. Malathion degradation by Trichoderma viride and a Pseudomonas species [J]. Science, 1966, 153(3741): 1278−1280. doi: 10.1126/science.153.3741.1278
[13]	SHI Y H, REN L, JIA Y, et al. Genome sequence of organophosphorus pesticide-degrading bacterium Pseudomonas stutzeri strain YC-YH₁ [J]. Genome Announcements, 2015, 3(2): 192. doi: 10.1128/genomea.00192-15
[14]	ERMAKOVA I T, SHUSHKOVA T V, SVIRIDOV A V, et al. Organophosphonates utilization by soil strains of Ochrobactrum anthropi and Achromobacter sp [J]. Archives of Microbiology, 2017, 199(5): 665−675. doi: 10.1007/s00203-017-1343-8
[15]	SUN L N, LIU H M, GAO X H, et al. Isolation of monocrotophos-degrading strain Sphingobiumsp. YW16 and cloning of its TnopdA [J]. Environmental Science and Pollution Research, 2018, 25(5): 4942−4950. doi: 10.1007/s11356-017-0718-3
[16]	顾欣, 刘文辉, 杨环羽, 等. 有机磷农药广谱降解菌A1A18菌株(Brevundimonas sp.)的筛选、鉴定与降解特性分析 [J]. 西北农业学报, 2019, 28(11):1896−1905. GU X, LIU W H, YANG H Y, et al. Screening, identification and degradation characteristics of a broad- spectrum organophosphorus pesticide-degrading bacteria strain A1A18 (Brevundimonas sp.) [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2019, 28(11): 1896−1905.(in Chinese)
[17]	ABO-AMER A. Biodegradation of diazinon by Serratia marcescens DI101 and its use in bioremediation of contaminated environment [J]. Journal of Microbiology and Biotechnology, 2011, 21(1): 71−80. doi: 10.4014/jmb.1007.07024
[18]	黄高凌, 蔡慧农, 曾琪, 等. 碱水解-分光光度法快速检测有机磷农药的研究 [J]. 集美大学学报(自然科学版), 2009, 14(4):366−371. HUANG G L, CAI H N, ZENG Q, et al. Study on an alkalinehydrolysis-spectrophotometry for detecting organophosphate pesticide [J]. Journal of Jimei University (Natural Science Edition), 2009, 14(4): 366−371.(in Chinese)
[19]	石成春. 有机磷农药曲霉生物降解特性及其动力学的研究[D]. 福州: 福州大学, 2005. SHI C C. Study on the characteristics and kinetics of biodegradation of organophosphate pesticides by the aspergillus[D]. Fuzhou: Fuzhou University, 2005.(in Chinese)
[20]	汪耀明, 陶玉贵, 叶连斌, 等. 毛细管电泳仪测定土壤中氧化乐果的含量 [J]. 安徽工程科技学院学报(自然科学版), 2007, 22(4):38−41. WANG Y M, TAO Y G, YE L B, et al. Determination of omethoate in soil by capillary electrophoresis [J]. Journal of Anhui University of Technology and Science (Natural Science Edition), 2007, 22(4): 38−41.(in Chinese)
[21]	杨慧. 有机磷农药降解菌的分离、鉴定及固定化研究[D]. 哈尔滨: 黑龙江大学, 2008. YANG H. Isolation, identification and immobilization of organophosphorus pesticide degrading bacteria[D]. Harbin: Helongjiang University, 2008.(in Chinese)
[22]	LU Y L, SONG S, WANG R S, et al. Impacts of soil and water pollution on food safety and health risks in China [J]. Environment International, 2015, 77: 5−15. doi: 10.1016/j.envint.2014.12.010
[23]	CHOUDRI B S, CHARABI Y. Pesticides and herbicides [J]. Water Environment Research, 2019, 91(10): 1342−1349. doi: 10.1002/wer.1227
[24]	VAN BRUGGEN A H C, HE M M, SHIN K, et al. Environmental and health effects of the herbicide glyphosate [J]. The Science of the Total Environment, 2018, 616/617: 255−268. doi: 10.1016/j.scitotenv.2017.10.309
[25]	UWIZEYIMANA H, WANG M, CHEN W P, et al. The eco-toxic effects of pesticide and heavy metal mixtures towards earthworms in soil [J]. Environmental Toxicology and Pharmacology, 2017, 55: 20−29. doi: 10.1016/j.etap.2017.08.001
[26]	RICHARDSON J R, FITSANAKIS V, WESTERINK R H S, et al. Neurotoxicity of pesticides [J]. Acta Neuropathologica, 2019, 138(3): 343−362. doi: 10.1007/s00401-019-02033-9
[27]	ISLAM F, WANG J, FAROOQ M A, et al. Potential impact of the herbicide 2, 4-dichlorophenoxyacetic acid on human and ecosystems [J]. Environment International, 2018, 111: 332−351. doi: 10.1016/j.envint.2017.10.020
[28]	JIN Y X, WU S S, ZENG Z Y, et al. Effects of environmental pollutants on gut microbiota [J]. Environmental Pollution, 2017, 222: 1−9. doi: 10.1016/j.envpol.2016.11.045
[29]	CYCON M, MROZIK A, PIOTROWSKA-SEGET Z. Bioaugmentation as a strategy for the remediation of pesticide-polluted soil: A review [J]. Chemosphere, 2017, 172: 52−71. doi: 10.1016/j.chemosphere.2016.12.129
[30]	HUANG Y C, XIAO L J, LI F Y, et al. Microbial degradation of pesticide residues and an emphasis on the degradation of cypermethrin and 3-phenoxy benzoic acid: A review [J]. Molecules, 2018, 23(9): 2313. doi: 10.3390/molecules23092313
[31]	CYCOŃ M, PIOTROWSKA-SEGET Z. Pyrethroid-degrading microorganisms and their potential for the bioremediation of contaminated soils: A review [J]. Frontiers in Microbiology, 2016, 7: 1463.
[32]	CHUN M, CHNGCHUN S, YANGHAO G, et al. Study on characteristics of biocometabolic removal of omethoate by the Aspergillus spp [J]. Water Research, 2004, 38(5): 1139−1146. doi: 10.1016/j.watres.2003.11.026
[33]	LI C K, MA Y Z, MI Z H, et al. Screening for Lactobacillus plantarum strains that possess organophosphorus pesticide-degrading activity and metabolomic analysis of phorate degradation [J]. Frontiers in Microbiology, 2018, 9: 2048. doi: 10.3389/fmicb.2018.02048