A TaqMan RT-PCR Method for Detecting Porcine Circovirus 3

CHEN Rujing; WU Xuemin; CHEN Qiuyong; CHE Yongliang; WANG Longbai; YAN Shan; ZHOU Lunjiang

doi:10.19303/j.issn.1008-0384.2020.07.007

Volume 35 Issue 7

Jul. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(7): 739-745

CHEN R J, WU X M, CHEN Q Y, et al. A TaqMan RT-PCR Method for Detecting Porcine Circovirus 3 [J]. Fujian Journal of Agricultural Sciences，2020，35（7）：739−745 doi: 10.19303/j.issn.1008-0384.2020.07.007

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CHEN R J, WU X M, CHEN Q Y, et al. A TaqMan RT-PCR Method for Detecting Porcine Circovirus 3 [J]. Fujian Journal of Agricultural Sciences，2020，35（7）：739−745 doi: 10.19303/j.issn.1008-0384.2020.07.007

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A TaqMan RT-PCR Method for Detecting Porcine Circovirus 3

doi: 10.19303/j.issn.1008-0384.2020.07.007

Institute of Animal Husbandry and Veterinary Medicine/Fujian Animal Disease Control Technology Development Center, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received Date: 2019-09-23
Rev Recd Date: 2020-05-05
Publish Date: 2020-07-31

Abstract

Abstract

Objective A TaqMan RT-PCR method was established for detecting porcine circovirus 3 （PCV3） infection in swine. Method Specific primers and probe were designed by Oligo 7 software targeting the Rep gene after genetic comparison. Result The established TaqMan RT-PCR method could detect 42.2 copies·uL⁻¹ with no positive signal on common porcine infectious diseases indicating high specificity of the methodology. On the constructed positive plasmids, the coefficients of variation for the intra- and inter-assays were less than 1.48% showing a high detection reproducibility. From the 193 clinical specimens collected in Fujian from 2014 to 2018, the newly developed method showed a high prevalence of PCV3 at 65.80% and high co-infection with PCV2 at 52.85%. Conclusion The established TaqMan RT-PCR method was made available for studying the function of Rep in PCV3.
- Porcine circovirus 3,
- Rep gene,
- TaqMan RT-PCR method,
- detection

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References(34)

References

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