Extraction and Composition of Polyphenols from Purple Cowpeas

WU Qiong; WANG Mingyue; GE Huilin; MA Chen; LYU Daizhu

doi:10.19303/j.issn.1008-0384.2020.06.014

Volume 35 Issue 6

Jun. 2020

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Fujian Journal of Agricultural Sciences > 2020 > 35(6): 673-681. > DOI: 10.19303/j.issn.1008-0384.2020.06.014

WU Q, WANG M Y, GE H L, et al. Extraction and Composition of Polyphenols from Purple Cowpeas [J]. Fujian Journal of Agricultural Sciences，2020，35（6）：673−681. DOI: 10.19303/j.issn.1008-0384.2020.06.014

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Extraction and Composition of Polyphenols from Purple Cowpeas

Analysis & Testing Center, Chinese Academy of Tropical Agricultural Sciences/Agricultural Ministry Laboratory for Tropical Crop Product Quality and Safety Risk Assessment/Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou, Hainan　571101, China

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Received Date: January 18, 2020
Revised Date: June 10, 2020

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Abstract

Objective Polyphenol compounds in purple cowpeas were solvent-extracted, purified, and then chemically analyzed by HPLC.
Method Single factor and orthogonal experiments were conducted to optimize the polyphenol extraction from purple cowpeas. Using polyphenol yield as the criterion, effects of solvent, solid-liquid ratio, temperature, and pH applied for the process were evaluated for optimization. The crude extract was purified using a Sephadex LH-20 macroporous resin. HPLC with external standards was employed for the chemical determination on the purified product.
Result The optimized process applied a peas-to-solvent ratio of 1 ∶ 5 (m ∶ V) with an 80% acetone solution to extract 3 times at 4^oC and pH 6 for 5 h per cycle to reach a maximum total polyphenols content of the gallic acid equivalent of 614 mg·100 g⁻¹. The correlation coefficients on the linear functions of the 6 polyphenols against their respective standards ranged from 0.995 9 to 0.999 9 with a limit of detection (LOD) of 0.1～1.0 mg·100 g⁻¹ and that of quantitation 0.2～2.5 mg·100 g⁻¹ on the HPLC test. The average recoveries on these compounds ranged from 74.3% to 107% with a relative standard deviation (RSDs) of 0.4～8.6%. The 6 polyphenols in the purple cowpeas thus determined averaged between 0.46 mg·100 g⁻¹ and 6.5 mg·100 g⁻¹, with rutin being the most abundant.ing the most abundant.
Conclusion The polyphenol extraction from purple cowpeas was optimized to deliver high yields on 6 monomer phenols, which could be accurately determined quantitatively by HPLC.
- Purple cowpea,
- total polyphenols,
- extraction and purification,
- composition analysis

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[1]	祖艳侠, 郭军, 梅燚, 等. 2个不同荚色豇豆品种的营养比较 [J]. 浙江农业科学, 2016, 57(2):292−293. ZU Y X, GUO J, MEI Y, et al. Nutritional comparison of two varieties of pod - colored cowpea [J]. <italic>Journal of Zhejiang Agricultural Sciences</italic>, 2016, 57(2): 292−293.(in Chinese)
[2]	张红梅, 许文静, 陈华涛, 等. 不同荚色豇豆品种花青素和营养成分含量变化分析 [J]. 南方农业学报, 2017, 48(6):1080−1085. DOI: 10.3969/j.issn.2095-1191.2017.06.23 ZHANG H M, XU W J, CHEN H T, et al. Variation of anthocyanin contents and nutrient component contents in yardlong bean varieties with different pod colors [J]. <italic>Journal of Southern Agriculture</italic>, 2017, 48(6): 1080−1085.(in Chinese) DOI: 10.3969/j.issn.2095-1191.2017.06.23
[3]	刘佳伟, 徐康, 杨效登, 等. 豇豆种子及豆芽多酚类物质的含量及抗氧化性比较分析研究 [J]. 齐鲁工业大学学报(自然科学版), 2017, 31(2):41−46. LIU J W, XU K, YANG X D, et al. Comparison research on the contents and antioxidant property of the phenolic compounds in seeds and sprouts of cowpea [J]. <italic>Journal of Qilu University of Technology(Natural Science Edition)</italic>, 2017, 31(2): 41−46.(in Chinese)
[4]	OJWANG L O, DYKES L, AWIKA J M. Ultra performance liquid chromatography–tandem quadrupole mass spectrometry profiling of anthocyanins and flavonols in cowpea (<italic>Vigna unguiculata</italic>) of varying genotypes [J]. <italic>Journal of Agricultural and Food Chemistry</italic>, 2012, 60(14): 3735−3744. DOI: 10.1021/jf2052948
[5]	刘素娟, 杨蒙蒙, 黄浩, 等. 高效液相色谱-二极管阵列联用同时测定果酒和果醋中10种酚类物质 [J]. 中国调味品, 2018, 43(12):151−153, 163. DOI: 10.3969/j.issn.1000-9973.2018.12.029 LIU S J, YANG M M, HUANG H, et al. Simultaneous determination of 10 phenolics in fruit wine and fruit vinegar by HPLC-PDA [J]. <italic>China Condiment</italic>, 2018, 43(12): 151−153, 163.(in Chinese) DOI: 10.3969/j.issn.1000-9973.2018.12.029
[6]	邓雅妮, 郭时印, 肖航, 等. 果蔬中不可萃取多酚的研究进展 [J]. 农产品加工, 2018(20):62−65. DENG Y N, GUO S Y, XIAO H, et al. Research progress in non-extractable polyphenols in fruits and vegetables [J]. <italic>Farm Products Processing</italic>, 2018(20): 62−65.(in Chinese)
[7]	夏婷, 赵超亚, 杜鹏, 等. 食品中多酚类化合物种类、提取方法和检测技术研究进展 [J]. 食品与发酵工业, 2019, 45(5):231−238. XIA T, ZHAO C Y, DU P, et al. Research progress on classification, extraction, and detection of polyphenols in foods [J]. <italic>Food and Fermentation Industries</italic>, 2019, 45(5): 231−238.(in Chinese)
[8]	薛宏坤, 谭佳琪, 赵月明, 等. 树莓果渣总花色苷和总多酚微波萃取工艺及组分分析 [J]. 精细化工, 2018, 43(12):151−153, 163. XUE H K, TAN J Q, ZHAO Y M, et al. Optimization of Microwave-Assisted Extraction of Total Anthocyanins and Polyphenols from Raspberry Pomace and Its Composition Analysis [J]. <italic>Fine Chemicals</italic>, 2018, 43(12): 151−153, 163.(in Chinese)
[9]	董科, 冷云, 何方婷, 等. 植物多酚及其提取方法的研究进展 [J]. 食品工业科技, 2019, 40(2):326−330. DONG K, LENG Y, HE F T, et al. Research progress of polyphenol and extraction methods in plants [J]. <italic>Science and Technology of Food Industry</italic>, 2019, 40(2): 326−330.(in Chinese)
[10]	赖瑞联, 陈瑾, 冯新, 等. 橄榄多酚类物质研究进展 [J]. 热带作物学报, 2018, 39(12):2532−2541. DOI: 10.3969/j.issn.1000-2561.2018.12.029 LAI R L, CHEN J, FENG X, et al. Research progress on polyphenol of <italic>Canarium album</italic>(lour.) raeusch [J]. <italic>Chinese Journal of Tropical Crops</italic>, 2018, 39(12): 2532−2541.(in Chinese) DOI: 10.3969/j.issn.1000-2561.2018.12.029
[11]	黎英, 曾珍清, 张薇, 等. 漳平水仙茶饼多酚的纯化及其体外活性研究 [J]. 热带作物学报, 2017, 38(8):1456−1463. DOI: 10.3969/j.issn.1000-2561.2017.08.014 LI Y, ZENG Z Q, ZHANG W, et al. Separation and purification of polyphenols from Zhangping Shuixian tea cake by macroporous resin and its vitro activity [J]. <italic>Chinese Journal of Tropical Crops</italic>, 2017, 38(8): 1456−1463.(in Chinese) DOI: 10.3969/j.issn.1000-2561.2017.08.014
[12]	陆广念, 朱志雄, 宋晓敏. 常见蔬菜抗氧化活性与总酚含量的研究 [J]. 食品科技, 2009, 34(9):68−71. LU G N, ZHU Z X, SONG X M. Antioxidant activity and total phenolic of vegetables commonly consumed [J]. <italic>Food Science and Technology</italic>, 2009, 34(9): 68−71.(in Chinese)
[13]	林宝妹, 邱珊莲, 郑开斌, 等. 嘉宝果叶片总多酚提取工艺优化及其体外降糖活性 [J]. 福建农业学报, 2019, 34(5):587−594. LIN B M, QIU S L, ZHENG K B, et al. Process optimization and <italic>in vitro</italic> hypoglycemic effect of polyphenols extracted from jaboticaba leaves [J]. <italic>Fujian Journal of Agricultural Sciences</italic>, 2019, 34(5): 587−594.(in Chinese)
[14]	张东峰, 陈家豪, 郭静, 等. 7种柑橘多酚、黄酮含量及其抗氧化活性比较研究 [J]. 食品研究与开发, 2019, 40(6):69−74. DOI: 10.3969/j.issn.1005-6521.2019.06.013 ZHANG D F, CHEN J H, GUO J, et al. Comparative study on the polyphenol, flavonoid and antioxidant activity of seven varieties of <italic>Citrus</italic> [J]. <italic>Food Research and Development</italic>, 2019, 40(6): 69−74.(in Chinese) DOI: 10.3969/j.issn.1005-6521.2019.06.013
[15]	吴琼, 王明月, 吕岱竹, 等. 紫皮豇豆多酚类物质的含量及其降糖活性研究 [J]. 中国调味品, 2019, 44(10):59−62. DOI: 10.3969/j.issn.1000-9973.2019.10.013 WU Q, WANG M Y, LYU D Z, et al. Study on polyphenols content and hypoglycemic activity of <italic>Vigna unguiculata</italic> [J]. <italic>China Condiment</italic>, 2019, 44(10): 59−62.(in Chinese) DOI: 10.3969/j.issn.1000-9973.2019.10.013
[16]	HAMMERSCHMIDT P A, PRATT D E. Phenolic antioxidants of dried soybeans [J]. <italic>Journal of Food Science</italic>, 1978, 43(2): 556−559. DOI: 10.1111/j.1365-2621.1978.tb02353.x
[17]	STRUMEYERDH, MALINMJ. Condensed tannins in grain sorghum isolation, fractionation and characterization [J]. <italic>Agric. Food Chem</italic>, 1975, 23: 909−914. DOI: 10.1021/jf60201a019
[18]	朱叶梅, 张雯, 杨少杰, 等. HPLC法测定茶叶中的7种多酚类化合物的含量 [J]. 云南化工, 2018, 45(9):66−68. DOI: 10.3969/j.issn.1004-275X.2018.09.029 ZHU Y M, ZHANG W, YANG S J, et al. Simultaneous determination of nine polyphenols colorants in paper-making reconstituted tobacco by HPLC [J]. <italic>Yunnan Chemical Technology</italic>, 2018, 45(9): 66−68.(in Chinese) DOI: 10.3969/j.issn.1004-275X.2018.09.029
[19]	隋华嵩, 杨燕, 侯英, 等. 紫红豇豆色素组成及体外抗氧化活性研究 [J]. 食品研究与开发, 2014, 35(24):34−38. DOI: 10.3969/j.issn.1005-6521.2014.24.009 SUI H S, YANG Y, HOU Y, et al. Studies on Primary Identification and in Vitro Antioxidant Activity of Pigments from Purple-red Cowpea [J]. <italic>Yunnan Chemical Technology</italic>, 2014, 35(24): 34−38.(in Chinese) DOI: 10.3969/j.issn.1005-6521.2014.24.009

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Extraction and Composition of Polyphenols from Purple Cowpeas

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