Characterization of OsBI-1L-8 Promoter in Oryza sativa L.

CHEN Rui; YU Fake; LI Qingxian; SHAN Zhen

doi:10.19303/j.issn.1008-0384.2020.05.002

Volume 35 Issue 5

May 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(5): 474-479

CHEN R, YU F K, LI Q X, et al. Characterization of OsBI-1L-8 Promoter in Oryza sativa L . [J]. Fujian Journal of Agricultural Sciences，2020，35（5）：474−479 doi: 10.19303/j.issn.1008-0384.2020.05.002

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CHEN R, YU F K, LI Q X, et al. Characterization of OsBI-1L-8 Promoter in Oryza sativa L . [J]. Fujian Journal of Agricultural Sciences，2020，35（5）：474−479 doi: 10.19303/j.issn.1008-0384.2020.05.002

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Characterization of OsBI-1L-8 Promoter in Oryza sativa L.

doi: 10.19303/j.issn.1008-0384.2020.05.002

CHEN Rui^{1, 2
,},
YU Fake^{1, 2},
LI Qingxian^{1, 2},
SHAN Zhen^{1, 2}

1.
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture, Fuzhou, Fujian　350003, China

Received Date: 2020-02-03
Rev Recd Date: 2020-04-23
Publish Date: 2020-05-01

Abstract

Abstract

Objective Structure and function of OsBI-1L-8 promoter were studied to decipher the mechanism of OsBI-1L genetic family involved in the life of Oryza sativa L. Method Length of pOsBI-1L-8 was predicted using the online software BDGP, Softberry, and Promoter 2.0. The cis-acting regulatory elements of pOsBI-1L-8 isolated from the genomic DNA of Nipponbare were analyzed based on the PlantCARE database. The pOsBI-1L-8::GUS vector was then constructed and transformed into rice callus, and spatiotemporal expression characterization by histochemical staining. Result The length of pOsBI-1L-8 was determined to be 1 258 bp by the predictive analysis and PCR amplification. The region consisted of several cis elements associated with light signal, response to anaerobic stress, and hormone regulation. The GUS reporter gene driven by pOsBI-1L-8 was expressed only during the heading period at the stem-stem junctions, root-stem junctions, and stamens. Conclusion pOsBI-1L-8 was a tissue-specific promoter. OsBI-1L-8 gene was postulated to play an important role in the growth and stamen development of a rice plant.
- rice,
- OsBI-1L-8,
- promoter,
- expression characterization,
- GUS activity

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References(22)

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