Spectrum-effect Relationship between HPLC Fingerprints and DPPH-scavenging Activities of Tea Catechins

LIN Qing-xia; WANG Li-li; YANG Jun-guo; SONG Zhen-shuo; CHEN Lin

doi:10.19303/j.issn.1008-0384.2020.02.012

Volume 35 Issue 2

Feb. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(2): 210-216

LIN Q X, WANG L L, YANG J G, et al. Spectrum-effect Relationship between HPLC Fingerprints and DPPH-scavenging Activities of Tea Catechins [J]. Fujian Journal of Agricultural Sciences，2020，35（2）：210−216 doi: 10.19303/j.issn.1008-0384.2020.02.012

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LIN Q X, WANG L L, YANG J G, et al. Spectrum-effect Relationship between HPLC Fingerprints and DPPH-scavenging Activities of Tea Catechins [J]. Fujian Journal of Agricultural Sciences，2020，35（2）：210−216 doi: 10.19303/j.issn.1008-0384.2020.02.012

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Spectrum-effect Relationship between HPLC Fingerprints and DPPH-scavenging Activities of Tea Catechins

doi: 10.19303/j.issn.1008-0384.2020.02.012

1.
Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou, Fujian　363000, China

Received Date: 2019-10-23
Rev Recd Date: 2019-12-26
Publish Date: 2020-02-01

Abstract

Abstract

Objective Correlation between the high-performance liquid chromatography (HPLC) fingerprints and the DPPH radical scavenging activities of catechins from varieties of teas was studied. Method From 5 categories of teas including green tea, white tea, northern Fujian oolong, southern Fujian oolong, and black tea, 25 specimens were collected and extracted with 70% methanol. The extracts were used to determine polyphenols content by the Folin-Ciocalteu method, the free radical scavenging activity by [1,1-diphenyl-2-picrylhydrazylradical2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl, DPPH] assay, and the fingerprints by HPLC for a principal component analysis. A correlation model between the HPLC fingerprints and DPPH radical scavenging activity was established by a partial least squares regression analysis and validated by the Pearson analysis. Result The HPLC fingerprints of the 25 specimens had 8 common compounds. The established regression model showed a coefficient of determination of R²=0.900 9. The 3 compounds, epigallocatechin gallate (EGCG), epigallocatechin (EGC), and epicatechin gallate (ECG), exhibited the strongest and significant correlations with DPPH radical scavenging activity. On the other hand, gallic acid (GA) correlated inversely with the scavenging activity. The Pearson analysis showed a result consistent with what the partial least squares regression test did. Conclusion It appeared that the antioxidant activity of catechins in the various teas could be satisfactorily predicted by HPLC fingerprints.
- catechins,
- antioxidant activities,
- high-performance liquid chromatography (HPLC),
- spectrum-effect relationship

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