<i>NPR1</i> Cloning and Expression during Development of Induced Systemic Resistance in <i>Oncidium hybridum</i>

ZHENG Shi-zhong; WANG Pei-yu; ZHANG Chun-liu; JIANG Sheng-tao; JIANG Jin-lan; YAN Pei-pei; YE Wei; LAI Zhong-xiong

doi:10.19303/j.issn.1008-0384.2020.02.004

Volume 35 Issue 2

Feb. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(2): 140-149

ZHENG S Z, WANG P Y, ZHANG C L, et al. NPR1 Cloning and Expression during Development of Induced Systemic Resistance in Oncidium hybridum [J]. Fujian Journal of Agricultural Sciences，2020，35（2）：140−149 doi: 10.19303/j.issn.1008-0384.2020.02.004

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ZHENG S Z, WANG P Y, ZHANG C L, et al. NPR1 Cloning and Expression during Development of Induced Systemic Resistance in Oncidium hybridum [J]. Fujian Journal of Agricultural Sciences，2020，35（2）：140−149 doi: 10.19303/j.issn.1008-0384.2020.02.004

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NPR1 Cloning and Expression during Development of Induced Systemic Resistance in Oncidium hybridum

doi: 10.19303/j.issn.1008-0384.2020.02.004

1.
College of Life Sciences, Ningde Normal University, Ningde, Fujian　352100, China
2.
Sanming Academy of Agricultural Sciences, Sanming, Fujian　365059, China
3.
Ningde Agricultural Bureau, Ningde, Fujian　352100, China
4.
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2019-12-02
Rev Recd Date: 2020-01-19
Publish Date: 2020-02-01

Abstract

Abstract

Objective Physiological role of NPR1 gene plays in the development of induced systemic resistance (ISR) in Oncidium hybridum was studied. Method NPR1 of the orchid was cloned using RACE technique, and bioinformatics and phylogenenetic analyses on the gene conducted. After co-cultured with Piriformospora indica or treated with salicylic acid (SA) or methyl jasmonate (MeJA) to induce ISR, the orchids were analyzed for the treatment effect against soft rot disease caused by Erwinia chrysanthemi. And, expressions of NPR1 during the treatments were investigated. Result Two full-length cDNA sequences were cloned. They were of a same type, one named OgNPR1-1 with a length of 1 804 bp encoding 555 amino acids, and the other, OgNPR1-2 1 902 bp encoding 556 amino acids. Their expressions were slightly upregulated by the inoculation of symbiotic P. indica. However, as both P. indica and E. chrysanthemi were present in the orchids, the expressions were significantly upregulated. It indicated that, in the presence of P. indica, the orchid plants became significantly more resistant to E. chrysanthemi inhibiting spread of the soft rot disease. On the other hand, the symbiosis of P. indica did not upregulated the expressions of the two genes more than the treatment by exogenous SA or MeJA on the orchids. Conclusion The expression of NPR1 in the orchids inoculated with the symbiotic P. indica was significantly upregulated in the presence of E. chrysanthemi. Although the increased expression by P. indica was lower than what induced by exogenous SA or MeJA hormone treatment, nonetheless, the orchids benefitted from the symbiosis and became more resistance to the soft rot disease.
- Oncidium hybridum,
- NPR1,
- induced systemic resistance,
- Piriformospora indica,
- Erwinia chrysanthemi

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References(51)

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