Microbial Community and Diversity in Specialty Teas from Fujian

HE Xiao-yun; RAO Qiu-hua; LIU Lan-ying; FU Jian-wei

doi:10.19303/j.issn.1008-0384.2020.01.005

Volume 35 Issue 1

Jan. 2020

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HE X Y, RAO Q H, LIU L Y, et al. Microbial Community and Diversity in Specialty Teas from Fujian [J]. Fujian Journal of Agricultural Sciences，2020，35（1）：28−37 doi: 10.19303/j.issn.1008-0384.2020.01.005

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HE X Y, RAO Q H, LIU L Y, et al. Microbial Community and Diversity in Specialty Teas from Fujian [J]. Fujian Journal of Agricultural Sciences，2020，35（1）：28−37 doi: 10.19303/j.issn.1008-0384.2020.01.005

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Microbial Community and Diversity in Specialty Teas from Fujian

doi: 10.19303/j.issn.1008-0384.2020.01.005

Institute of Quality Standards & Testing Technology for Agro-products, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou, Fujian　350003, China

Received Date: 2019-10-29
Rev Recd Date: 2020-01-09
Publish Date: 2020-01-01

Abstract

Abstract

Objective Diversity and distribution of the microbial communities in the specialty teas produced in Fujian were studied. Method Fifteen groups of well-known local teas in the province were collected for an analysis based on the high-throughput sequencing targeting 16S rRNA gene. Result A total of 11 472 OTUs encompassing 37 phyla, 95 classes, 150 orders, 207 families, and 277 genera were isolated from the specimens. In the 3 categories, Dahongpao had the greatest richness on species; the Huacha was the most diversified; and, as oolong teas, Dahongpao and Tieguanyin had a similar microbial structure. In 10 groups of Dahongpao samples, the dominant genera were Lactococcus, Bifidobacterium, and Bacteroides; that in 2 groups of Huacha, Bacteroides; and those in 3 groups of Tieguanyin, Lactococcus and Bifidobacterium. Five of the top 10 dominant genera in the teas were lactic acid bacteria. According to the co-occurrence analysis on network, the lactic acid bacteria exhibited a synergistic growth relationship with most of the other significantly associated species. Conclusion Bacilli and Actinobacteria were the predominant bacteria found in Dahongpao and Tieguanyin, while Clostridia and Bacteroidia in Huacha. A bacterial taxonomy analysis indicated that tea category differentiated the structure of their bacterial communities. Overall, lactic acid bacteria dominantly existed in these teas produced in Fujian.
- tea,
- microbial community structure,
- 16S rRNA,
- high throughput sequencing

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References(47)

References

[1]	ZHANG L, HO C T, ZHOU J, et al. Chemistry and Biological Activities of Processed Camellia sinensis Teas: A Comprehensive Review [J]. Comprehensive Reviews in Food Science and Food Safety, 2019, 18(5): 1474−1495. doi: 10.1111/1541-4337.12479
[2]	WEI C, YANG H, WANG S, et al. Draft genome sequence of Camellia sinensis var. sinensis provides insights into the evolution of the tea genome and tea quality [J]. Proceedings of the National Academy of Sciences, 2018, 115(18): E4151−E4158. doi: 10.1073/pnas.1719622115
[3]	马存强, 杨超, 周斌星, 等. 微生物对茶叶中嘌呤生物碱代谢的研究进展 [J]. 食品科学, 2014, 35(21):292−296. doi: 10.7506/spkx1002-6630-201421057 MA C Q, YANG C, ZHOU B X, et al. Recent progress in microbial metabolism of purine alkaloids in fermented tea [J]. Food science, 2014, 35(21): 292−296.(in Chinese) doi: 10.7506/spkx1002-6630-201421057
[4]	陈林, 项丽慧, 王丽丽, 等. 乌龙茶和绿茶品种茶鲜叶儿茶素类和嘌呤碱HPLC指纹图谱特征比较 [J]. 福建农业学报, 2018, 33(1):21−28. CHEN L, XIANG L H, WANG L L, et al. HPLC Profiling of Catechins and Purine Alkaloids in Leaves of Oolong and Green Tea Cultivars [J]. Fujian Journal of Agricultural Sciences, 2018, 33(1): 21−28.(in Chinese)
[5]	LI H L, JAYAWARDENA R S, XU W, et al. Lasiodiplodia theobromae and L. pseudotheobromae causing leaf necrosis on Camellia sinensis in Fujian Province, China [J]. Canadian Journal of Plant Pathology, 2019, 41(2): 277−284. doi: 10.1080/07060661.2019.1569559
[6]	姚清华, 张居德, 苏德森, 等. 福建省茶叶主产区茶叶质量安全管理与隐患点比较分析 [J]. 福建农业学报, 2014, 29(4):393−397. doi: 10.3969/j.issn.1008-0384.2014.04.018 YAO Q H, ZHANG J D, SU D S, et al. Quality and Safety Management on Tea in Fujian [J]. Fujian Journal of Agricultural Sciences, 2014, 29(4): 393−397.(in Chinese) doi: 10.3969/j.issn.1008-0384.2014.04.018
[7]	许凌, 周卫龙, 宿迷菊. 茶叶微生物指标的现状与展望 [J]. 中国茶叶, 2012(7):14−16. doi: 10.3969/j.issn.1000-3150.2012.07.008 XU L, ZHOU W L, SU M J. Present situation and prospect of microbial indicators of tea [J]. China tea, 2012(7): 14−16.(in Chinese) doi: 10.3969/j.issn.1000-3150.2012.07.008
[8]	BORAH A, DAS R, MAZUMDAR R, et al. Culturable endophytic bacteria of Camellia species endowed with plant growth promoting characteristics [J]. Journal of Applied Microbiology, 2019, 127(3): 825−844. doi: 10.1111/jam.14356
[9]	MADHAB M, BHATTACHARYYA P N, BEGUM R, et al. Microbial distribution and contamination in black tea [J]. Two and a Bud, 2019, 64(1): 26−31.
[10]	谌滢, 李适, 刘仲华, 等. 黑茶陈化机制研究进展 [J]. 湖南农业科学, 2016(12):118−122. CHEN Y, LI S, LIU Z H, et al. Research progress on the aging mechanism of black tea [J]. Hunan Agricultural Sciences, 2016(12): 118−122.(in Chinese)
[11]	徐正刚, 吴良, 刘石泉, 等. 黑茶发酵过程中微生物多样性研究进展 [J]. 生物学杂志, 2019, 36(3):92−95. doi: 10.3969/j.issn.2095-1736.2019.03.092 XU Z G, WU L, LIU S Q, et al. Review for development of microbial diversity during dark tea fermentation period [J]. Journal of Biology, 2019, 36(3): 92−95.(in Chinese) doi: 10.3969/j.issn.2095-1736.2019.03.092
[12]	罗红玉, 钟应富, 袁林颖, 等. 微生物在传统茶加工中的应用研究进展 [J]. 福建茶叶, 2013, 35(1):13−16. doi: 10.3969/j.issn.1005-2291.2013.01.004 LUO H Y, ZHONG Y F, YUAN L Y, et al. Research progress on the application of microorganisms in traditional tea processing [J]. Tea in Fujian, 2013, 35(1): 13−16.(in Chinese) doi: 10.3969/j.issn.1005-2291.2013.01.004
[13]	TIAN J, ZHU Z, WU B, et al. Bacterial and Fungal Communities in Pu’er Tea Samples of Different Ages [J]. Journal of Food Science, 2013, 78(8): M1249−M1256. doi: 10.1111/1750-3841.12218
[14]	刘石泉, 胡治远, 赵运林. 基于DGGE技术的茯砖茶发花过程细菌群变化分析 [J]. 生态学报, 2014, 34(11):3007−3015. LIU S Q, HU Z Y, ZHAO Y L. Analysis of bacterial flora during the fahua fermentation process of fuzhuan brick tea production based on DGGE technology [J]. Acta Ecologica Sinica, 2014, 34(11): 3007−3015.(in Chinese)
[15]	RUI Y, WAN P, CHEN G, et al. Analysis of bacterial and fungal communities by Illumina MiSeq platforms and characterization of Aspergillus cristatus in Fuzhuan brick tea [J]. LWT, 2019, 110: 168−174. doi: 10.1016/j.lwt.2019.04.092
[16]	LI Q, CHAI S, LI Y D, et al. Biochemical components associated with microbial community shift during the pile-fermentation of primary dark tea [J]. Frontiers in Microbiology, 2018, 9: 1509. doi: 10.3389/fmicb.2018.01509
[17]	CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al. QIIME allows analysis of high-throughput community sequencing data [J]. Nature Methods, 2010, 7(5): 335−336. doi: 10.1038/nmeth.f.303
[18]	ROGNES T, FLOURI T, NICHOLS B, et al. VSEARCH: a versatile open source tool for metagenomics [J]. PeerJ, 2016, 4: e2584. doi: 10.7717/peerj.2584
[19]	SEGATA N, IZARD J, WALDRON L, et al. Metagenomic biomarker discovery and explanation [J]. Genome Biology, 2011, 12(6): R60. doi: 10.1186/gb-2011-12-6-r60
[20]	李艳春, 林忠宁, 陆烝, 等. 茶园间作灵芝对土壤细菌多样性和群落结构的影响 [J]. 福建农业学报, 2019, 34(6):690−696. LI Y C, LIN Z N, LU Z, et al. Microbial Diversity and Community Structure in Soil under Tea Bushes-Ganoderma lucidum Intercropping [J]. Fujian Journal of Agricultural Sciences, 2019, 34(6): 690−696.(in Chinese)
[21]	ALOTAIBI K, RAYWARD-SMITH V, IGLESIA B D L. Nonmetric multidimensional scaling: A perturbation model for privacy-preserving data clustering [J]. Statistical Analysis & Data Mining the Asa Data Science Journal, 2014, 7(3): 175−193.
[22]	DHARIWAL A, CHONG J, HABIB S, et al. MicrobiomeAnalyst: a web-based tool for comprehensive statistical, visual and meta-analysis of microbiome data [J]. Nucleic Acids Research, 2017, 45(W1): W180−W188. doi: 10.1093/nar/gkx295
[23]	FAUST K, RAES J. Microbial interactions: from networks to models [J]. Nature Reviews Microbiology, 2012, 10(8): 538−550. doi: 10.1038/nrmicro2832
[24]	FUHRMAN J A, CRAM J A, NEEDHAM D M. Marine microbial community dynamics and their ecological interpretation [J]. Nature Reviews Microbiology, 2015, 13(3): 133−146. doi: 10.1038/nrmicro3417
[25]	MOLLOY S. Environmental microbiology: disentangling syntrophy [J]. Nature Reviews Microbiology, 2014, 12(1): 7.
[26]	HIBBING M E, FUQUA C, PARSEK M R, et al. Bacterial competition: surviving and thriving in the microbial jungle [J]. Nature Reviews Microbiology, 2010, 8(1): 15−25. doi: 10.1038/nrmicro2259
[27]	LI H, LI M, YANG X, et al. Microbial diversity and component variation in Xiaguan Tuo Tea during pile fermentation [J]. PLoS ONE, 2018, 13(2): e0190318. doi: 10.1371/journal.pone.0190318
[28]	XU Q, SUN M, NING J, et al. The Core Role of Bacillus subtilis and Aspergillus fumigatus in Pile-Fermentation Processing of Qingzhuan Brick Tea [J]. Indian Journal of Microbiology, 2019, 59(3): 288−294. doi: 10.1007/s12088-019-00802-4
[29]	XU A, WANG Y, WEN J, et al. Fungal community associated with fermentation and storage of Fuzhuan brick-tea [J]. International Journal of Food Microbiology, 2011, 146(1): 14−22. doi: 10.1016/j.ijfoodmicro.2011.01.024
[30]	MAO Y, WEI B Y, TENG J W, et al. Analyses of fungal community by Illumina MiSeq platforms and characterization of Eurotium species on Liupao tea, a distinctive post-fermented tea from China [J]. Food Research International, 2017, 99: 641−649. doi: 10.1016/j.foodres.2017.06.032
[31]	ZHANG W, YANG R, FANG W, et al. Characterization of thermophilic fungal community associated with pile fermentation of Pu-erh tea [J]. International Journal of Food Microbiology, 2016, 227: 29−33. doi: 10.1016/j.ijfoodmicro.2016.03.025
[32]	赵仁亮, 胥伟, 吴丹, 等. 基于Illumina MiSeq技术分析不同地域加工的茯砖茶中微生物群落多样性 [J]. 生态学杂志, 2017, 36(7):1865−1876. ZHAO R L, XU W, WU D, et al. Microbial community diversity of Fu brick tea produced in different regions by Illumina MiSeq technology [J]. Chinese Journal of Ecology, 2017, 36(7): 1865−1876.(in Chinese)
[33]	FU J Y, LV H P, CHEN F. Diversity and Variation of Bacterial Community Revealed by MiSeq Sequencing in Chinese Dark Teas [J]. PLoS ONE, 2016, 11(9): e0162719. doi: 10.1371/journal.pone.0162719
[34]	宁伟泽, 徐军. 金花普洱茶微生物组成谱的ITS和16sRNA基因文库研究 [J]. 中国茶叶, 2018, 40(5):63−66. doi: 10.3969/j.issn.1000-3150.2018.05.010 NING W Z, XU J. Study on ITS and 16sRNA gene library of microbial composition spectrum of jinhua puer tea [J]. China Tea, 2018, 40(5): 63−66.(in Chinese) doi: 10.3969/j.issn.1000-3150.2018.05.010
[35]	MUDAU F N, SALEHI B, SHARIFI-RAD M, et al. Understanding Camellia sinensis using Omics technologies along with endophytic bacteria and environmental roles on metabolism: A review [J]. Applied Sciences (Switzerland), 2019, 9(2): 281. doi: 10.3390/app9020281
[36]	AZAM M W, KHAN A U. Updates on the pathogenicity status of Pseudomonas aeruginosa [J]. Drug Discovery Today, 2019, 24(1): 350−359. doi: 10.1016/j.drudis.2018.07.003
[37]	SHARMA A K, PANDIT J, GAUTAM V, et al. Optimization of culture conditions for maximizing Extracellular Organophosphorus hydrolase activity in pseudomonas strains with Chlorpyrifos degradation potential [J]. Journal of Pharmacognosy and Phytochemistry, 2019, SP1: 322−326.
[38]	WALLACE R L, HIRKALA D L, NELSON L M. Postharvest biological control of blue mold of apple by Pseudomonas fluorescens during commercial storage and potential modes of action [J]. Postharvest Biology & Technology, 2017, 133: 1−11.
[39]	CHEN H, HAO Z, WANG Q, et al. Occurrence and risk assessment of organophosphorus pesticide residues in Chinese tea [J]. Human and Ecological Risk Assessment: An International Journal, 2016, 22(1): 28−38. doi: 10.1080/10807039.2015.1046420
[40]	SHARMA A, PANDIT J, SHARMA R, et al. Biodegradation of Chlorpyrifos by Pseudomonas Resinovarans Strain AST2. 2 Isolated from Enriched Cultures [J]. Current World Environment, 2016, 11(1): 267−278. doi: 10.12944/CWE.11.1.33
[41]	DONG J, BIAN Y, LIU F, et al. Storage stability improvement of organophosphorus insecticide residues on representative fruit and vegetable samples for analysis [J]. Journal of Food Processing and Preservation, 2019: e14048.
[42]	王小军, 张绵松, 袁文鹏, 等. 液态发酵茶饮料菌株分离鉴定及发酵特征研究 [J]. 农产品加工(学刊), 2010(8):30−35. WANG X J, ZHANG M S, YUAN W P, et al. Isolation and identification and fermentation characteristic of microorganism used for liquid fermented tea drink [J]. The Processing of Agricultural Products, 2010(8): 30−35.(in Chinese)
[43]	SONG A A L, IN L L A, LIM S H E, et al. A review on Lactococcus lactis: from food to factory [J]. Microbial cell factories, 2017, 16(1): 55. doi: 10.1186/s12934-017-0669-x
[44]	杨浩, 杨晓妮, 张国珍, 等. 窖水中微生物降解污染物的关键细菌 [J]. 环境科学, 2018, 39(10):376−387. YANG H, YANG X N, ZHANG G Z, et al. Key bacteria for microbial degradation of pollutants in cellar water [J]. Environmental Science, 2018, 39(10): 376−387.(in Chinese)
[45]	CHEN Y, LI Q, XIA C, et al. Effect of selenium supplements on the antioxidant activity and nitrite degradation of lactic acid bacteria [J]. World Journal of Microbiology and Biotechnology, 2019, 35(4): 61. doi: 10.1007/s11274-019-2609-x
[46]	YUE X, YU G, LU Y, et al. Effect of dissolved oxygen on nitrogen removal and the microbial community of the completely autotrophic nitrogen removal over nitrite process in a submerged aerated biological filter [J]. Bioresource Technology, 2018, 254: 67−74. doi: 10.1016/j.biortech.2018.01.044
[47]	XU S, LU W J, MUSTAFA M F, et al. Co-existence of anaerobic ammonium oxidation bacteria and denitrifying anaerobic methane oxidation bacteria in sewage sludge: community diversity and seasonal dynamics [J]. Microbial Ecology, 2017, 74(4): 832−840.