Anther Culture of Blast-resistant Indica-japonica Hybrid, Yunyin

WEI Yi-dong; XIE Xiao-ping; XIE hong-guang; WANG Ke-wu; QÜ Meng-yu; JIANG Jia-huan; ZHENG Yan-mei; XIE Hua-an; ZHANG Jian-fu

doi:10.19303/j.issn.1008-0384.2020.01.001

Volume 35 Issue 1

Jan. 2020

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WEI Y D, XIE X P, XIE H G, et al. Anther Culture of Blast-resistant Indica-japonica Hybrid, Yunyin [J]. Fujian Journal of Agricultural Sciences，2020，35（1）：1−5 doi: 10.19303/j.issn.1008-0384.2020.01.001

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WEI Y D, XIE X P, XIE H G, et al. Anther Culture of Blast-resistant Indica-japonica Hybrid, Yunyin [J]. Fujian Journal of Agricultural Sciences，2020，35（1）：1−5 doi: 10.19303/j.issn.1008-0384.2020.01.001

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Anther Culture of Blast-resistant Indica-japonica Hybrid, Yunyin

doi: 10.19303/j.issn.1008-0384.2020.01.001

WEI Yi-dong^{1, 2
,},
XIE Xiao-ping^{1, 2, 3
,},
XIE hong-guang^{1, 2},
WANG Ke-wu^{1, 2, 3},
QÜ Meng-yu^{1, 2},
JIANG Jia-huan^{1, 2},
ZHENG Yan-mei^{1, 2},
XIE Hua-an^{1, 2
,
,},
ZHANG Jian-fu^{1, 2
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350019, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affair, China/Fuzhou Branch, National Rice Improvement Center of China/Incubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology/Base of South-China, National Key Laboratory of Hybrid Rice/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian　350003, China
3.
Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2019-10-06
Rev Recd Date: 2019-12-13
Publish Date: 2020-01-01

Abstract

Abstract

Objective To improve callus induction and seedling formation rates of the anther culture for an expedited and stable breeding program utilizing blast-resistant Indica-japonica hybrid rice Yunyin as a parent, an optimized medium and conditions for the auxiliary breeding technology in creating new materials and varieties were investigated. Method The young spikes of F₁ hybrid of Yunyin and a conventional Indica rice Yuejingsimiao were disinfected and inoculated. From 12 formulations of base media, carbon sources, and 2,4-D concentrations as well as culture conditions, an optimal anther cultivation according to the rates of callus induction, green seedling, seedling yield, and anther cultivation performance was selected. Result The maximum anther cultivation performance using the NB medium was merely 0.52%, whereas, M8 significantly higher at 0.90%–1.92%. The M8 medium with sucrose for carbon source reached 11.32% on the performance at the early stage of callus induction. The use of 1.0 mg·L⁻¹ or 2 mg·L⁻¹ 2,4-D in the medium did not differ significantly on the performance, i.e., 1.89% vs 1.92%. A peaked green seedling rate up to 18.3% was achieved when 0.2 cm-sized calluses were used for the differentiation. Conclusion The optimal culture conditions of Yunyin Indica-japonica hybrid anthers were to use calluses inducing on M8 medium with added 50 g·L⁻¹ sucrose and 1–2 mg·L⁻¹ 2,4-D, then, when grew to 0.2 cm in size, transferring to a differentiation medium.
- anther culture,
- Indica-japonica hybrid,
- Yunyin,
- blast-resistant variety

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References(15)

References

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