Inhibitory Effects of <i>Laminaria Japonica</i> Polysaccharides on Proliferation and Antioxidant Activities of Vascular Smooth Muscle Cells

PENG Zhen-fei; LI Yong-ning; WEI Bi-na; LIN Bi-zhu

doi:10.19303/j.issn.1008-0384.2019.12.014

Volume 34 Issue 12

Dec. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(12): 1457-1462

PENG Z F, LI Y N, WEI B N, et al. Inhibitory Effects of Laminaria Japonica Polysaccharides on Proliferation and Antioxidant Activities of Vascular Smooth Muscle Cells [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1457−1462. doi: 10.19303/j.issn.1008-0384.2019.12.014

Citation:

PENG Z F, LI Y N, WEI B N, et al. Inhibitory Effects of Laminaria Japonica Polysaccharides on Proliferation and Antioxidant Activities of Vascular Smooth Muscle Cells [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1457−1462. doi: 10.19303/j.issn.1008-0384.2019.12.014

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Inhibitory Effects of Laminaria Japonica Polysaccharides on Proliferation and Antioxidant Activities of Vascular Smooth Muscle Cells

doi: 10.19303/j.issn.1008-0384.2019.12.014

Fujian Health College, Fuzhou, Fujian　350101, China

Received Date: 2019-10-28
Rev Recd Date: 2019-12-09
Publish Date: 2019-12-01

Abstract

Abstract

Objective Effects of Laminaria japonicapolysaccharides on the proliferation and lipid peroxide production of H₂O₂-induced vascular smooth muscle cells (VSMCs) were examined in vitro for further understanding on the metabolic mechanism. Method Proliferation of VSMCs was artificially induced by using 50 µmol·L⁻¹ H₂O₂. Inhibitory rate on the proliferation by the L. japonica polysaccharides were determined by a methyl thiazolyl tetrazolium (MTT) assay. Morphological changes on VSMCs were observed under a microscope. Malondialdehyde (MDA) was employed as an indicator of the lipid peroxide production in VSMCs. Result A significant inhibition effect of the polysaccharides on the H₂O₂-induced VSMC proliferation with the greatest rate of 73.56% was shown by MTT. Morphologically, the number of VSMCs significantly decreased after an exposure to the polysaccharide. The MDA analysis indicated that the lipid peroxide production by VSMCs significantly declined after the treatment with L. japonica polysaccharides. Conclusion The L. japonica polysaccharides significantly inhibited the proliferation and lipid peroxide production in the H₂O₂-induced VSMCs.
- Laminaria japonica polysaccharides,
- vascular smooth muscle cell,
- H₂O₂-induced model,
- cellular proliferation,
- lipid peroxide

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References(23)

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