Molecular Cloning and Functional Analysis of UDP N-acetylglucosamine Pyrophosphorylases Gene in <i>Frankliniella occidentalis</i>

LU Cheng-cong; LI Heng; ZHANG Xiang-qin; WANG Liang; CHEN Yi-xin; TIAN Hou-jun; LIN Shuo; ZHANG Jie; CHEN Yong; WEI Hui

doi:10.19303/j.issn.1008-0384.2019.12.009

Volume 34 Issue 12

Dec. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(12): 1419-1425

LU C C, LI H, ZHANG X Q, et al. Molecular Cloning and Functional Analysis of the UDP N-acetylglucosamine Pyrophosphorylases Gene in the Frankliniella occidentalis [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1419−1425. doi: 10.19303/j.issn.1008-0384.2019.12.009

Citation:

LU C C, LI H, ZHANG X Q, et al. Molecular Cloning and Functional Analysis of the UDP N-acetylglucosamine Pyrophosphorylases Gene in the Frankliniella occidentalis [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1419−1425. doi: 10.19303/j.issn.1008-0384.2019.12.009

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Molecular Cloning and Functional Analysis of UDP N-acetylglucosamine Pyrophosphorylases Gene in Frankliniella occidentalis

doi: 10.19303/j.issn.1008-0384.2019.12.009

LU Cheng-cong^1
,,
LI Heng^{1, 2},
ZHANG Xiang-qin^{1, 2},
WANG Liang^{1, 2},
CHEN Yi-xin¹,
TIAN Hou-jun¹,
LIN Shuo¹,
ZHANG Jie³,
CHEN Yong¹,
WEI Hui^{1, 2
,
,}

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fuzhou Scientific Observing and Experimental Station of Crop Pests of Ministry of Agriculture and Rural Affaris, Fuzhou, Fujian　350003, China
2.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, Fujian　350002, China
3.
Biotechnology and Germplasm Resource Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Agricultural Biotechnology/Key Laboratory for Gene Resources and Germplasm Innovation of Southwest Crops, Ministry of Agriculture and Rural Affairs, Kunming, Yunnan　650223, China

Received Date: 2019-10-12
Rev Recd Date: 2019-11-29
Publish Date: 2019-12-01

Abstract

Abstract

Objective Effects of UDP N-acetylglucosamine pyrophosphorylases (UAP) on the growth and development of Frankliniella occidentalis were studied. Method Based on a transcriptome dataset, an open reading frame (ORF) of UAP gene of F. occidentalis (FoccUAP) was cloned. Expressions of the FoccUAP in different body tissues and growth stages of the insect were detected by RT-qPCR. Effects of UAP gene on the growth and development were examined by microinjecting dsRAN in the 2^nd instars (eclosion about 24 h) of F. occidentalis. Result The full-length of FoccUAP ORF was 1 545 bp and encoded 514 amino acids with a predicted molecular mass of 56.738 kDa. FoccUAP contained the conserved domain of glyco-tranf-GTA-type super family, which had a close evolutionary relationship with the orthologues in Isoptera, Blattaria, Orthoptera, and Hemiptera. The RT-qPCR results showed that FoccUAP was highly expressed in the head and abdomen, detected in the antenna, thorax and leg, and found in all developmental stages of the insect. The relative transcript levels of FoccUAP in the 2^nd instar, pupa, adult-24 h, adult-48 h, adult-168 h, and adult-240 h were 1.30-, 2.50-, 1.56-, 2.0-, 2.43-, and 2.56-fold, respectively, of those in the 1^st instar. Further examination on the RNAi showed that the injection of dsUAP into the 2^nd instar F. occidentalis significantly decreased the eclosion rate (36.3%) and the survival rate (5.0% in 120 h after microinjection) as compared to dsGFP controls. Moreover, the dsUAP treatment also induced abnormal phenotypes on wings and abdomen–thorax of the thrips. Conclusion UAP played a significant role in the growth and development of F. occidentalis.
- Frankliniella occidentalis,
- UDP N-acetylglucosamine pyrophosphorylases,
- chitin biosynthesis,
- RNAi

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References(22)

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