Genetic Diversity on Selected Agronomic Traits of Arabica Coffee Germplasm

YAN Lin; CHEN Ting; HUANG Li-fang; LI Jin-hong; MA Guan-run; WANG Xiao-yang; DONG Yun-ping; LONG Yu-zhou; LI Xue-jun

doi:10.19303/j.issn.1008-0384.2019.12.004

Volume 34 Issue 12

Dec. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(12): 1379-1387

YAN L, CHEN T, HUANG L F, et al. Genetic Diversity on Selected Agronomic Traits of Arabica Coffee Germplasm [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1379−1387. doi: 10.19303/j.issn.1008-0384.2019.12.004

Citation:

YAN L, CHEN T, HUANG L F, et al. Genetic Diversity on Selected Agronomic Traits of Arabica Coffee Germplasm [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1379−1387. doi: 10.19303/j.issn.1008-0384.2019.12.004

Citation:

PDF( 641 KB)

Genetic Diversity on Selected Agronomic Traits of Arabica Coffee Germplasm

doi: 10.19303/j.issn.1008-0384.2019.12.004

1.
Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Science/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan　571533, China
2.
College of Tropical Crops, Yunnan Agricultural University, Puer, Yunnan　665099, China
3.
Dehong Tropical Agriculture Institute of Yunnan, Ruili, Yunnan　678600, China

Received Date: 2019-08-12
Rev Recd Date: 2019-09-30
Publish Date: 2019-12-01

Abstract

Abstract

Objective Diversity on the agronomic traits relating to breeding purpose of arabica coffee germplasms in collection was studied. Method From 60 arabica coffee germplasms, 8 agronomic and 5 quality traits were targeted and subjected them to the correlation, principal component, and cluster analyses for the genetic diversity study. Result Among the 8 agronomic traits, the fresh/dry ratio had the highest coefficient of variation of 25.15% followed by fresh fruit weight of 19.85%, while green bean rate of 1.97% being the lowest. Seed length ranked the highest on the genetic diversity indices. On the 5 quality traits, the chlorogenic acid content showed the highest coefficient of variation of 22.68% followed by fat of 12.40%, and protein of 5.20% being the lowest of all. Chlorogenic acid also had the greatest genetic diversity index of 2.07 followed by fat of 2.06 and protein of 2.05. The dry bean weight of the germplasms significantly correlated with their green bean rate; the seed size with 100-seed weight, green bean rate and protein; the 100-seed weight with green bean rate, protein and fat; and, the green bean rate with protein and fat. The contents of protein and fat showed an extremely significant correlation; whereas, those of caffeine and sucrose an extremely significant inverse correlation. The principal component analysis indicated the top 4 main components (i.e., PC1-PC4) included the 12 agronomic traits and had a combined contribution of 69.17% of the total constituting the major factors that affected the diversity. A cluster analysis classified the 60 germplasms into two groups, 53 in Group I and 7 in Group II. Group II was high on the fresh fruit weight, especially, Subgroup II-2, which exhibited high fresh and dry bean weights. Conclusion The arabica coffee germplasms under study were rich in diversity. The desirable genes could be preserved by domestication, hybridization, grafting and/or molecular breeding. Subgroup II-2, being high on fresh and dry bean weights, could conceivably be used as a parent for new variety breeding.
- Arabica coffee,
- agronomic traits,
- genetic diversity,
- correlation analysis,
- principal component analysis,
- cluster analysis

FullText(HTML)

References(29)

References

[1]	FAO Statistics[EB/OL]. (2019-01-18)[2019-08-12]. http://faostat.fao.org.
[2]	VAN DER VOSSEN H, BERTRAND B, CHARRIER A. Next generation variety development for sustainable production of Arabica coffee (Coffea arabica L.): a review [J]. Euphytica, 2015, 204(2): 243−256. doi: 10.1007/s10681-015-1398-z
[3]	赵青云, 普浩杰, 王秋晶, 等.咖啡果皮不同堆沤处理养分含量及对咖啡植株生长的影响[J/OL]. 热带作物学报, 2019:1-8[2019-09-21]. http://kns.cnki.net/kcms/detail/46.1019.s.20190920.1323.006.html. ZHAO Q Y, PU H J, WANG Q J, et al.Nutrient contents of coffee peel with different composting treatments and its effects on coffee plant growth[J/OL]. Chinese Journal of Tropical Crops, 2019:1-8[2019-09-21].http://kns.cnki.net/kcms/detail/46.1019.s.20190920.1323.006.html. (in Chinese)
[4]	周华, 张洪波, 夏红云, 等. 咖啡种质资源多样性研究 [J]. 中国热带农业, 2015(5):23−27. doi: 10.3969/j.issn.1673-0658.2015.05.007 ZHOU H, ZHANG H B, XIA H Y, et al. Genetic diversity analysis of coffee germlasms [J]. China Tropical Agriculture, 2015(5): 23−27.(in Chinese) doi: 10.3969/j.issn.1673-0658.2015.05.007
[5]	白学慧, 夏红云, 李锦红, 等. 咖啡种质资源抗锈性初步鉴定 [J]. 热带农业科学, 2014, 34(7):60−64. doi: 10.3969/j.issn.1009-2196.2014.07.012 BAI X H, XIA H Y, LI J H, et al. Resistance identification of coffee germplasm resources to rust [J]. Chinese Journal of Tropical Agriculture, 2014, 34(7): 60−64.(in Chinese) doi: 10.3969/j.issn.1009-2196.2014.07.012
[6]	YAN L, OGUTU C, HUANG L F, et al. Genetic diversity and population structure of coffee germplasm collections in China revealed by ISSR markers [J]. Plant Molecular Biology Reporter, 2019, 37(3): 204−213. doi: 10.1007/s11105-019-01148-3
[7]	闫林, 黄丽芳, 王晓阳, 等. 咖啡种质资源遗传多样性的ISSR分析 [J]. 南方农业学报, 2019, 50(3):491−499. YAN L, HUANG L F, WANG X Y, et al. Genetic diversity of coffee gemeplasms by ISSR analysis [J]. Journal of Southern Agriculture, 2019, 50(3): 491−499.(in Chinese)
[8]	闫林, 黄丽芳, 王晓阳, 等. 基于ISSR标记的咖啡资源遗传多样性分析 [J]. 热带作物学报, 2019, 40(2):300−307. YAN L, HUANG L F, WANG X Y, et al. Genetic diversity of coffee germplasms by ISSR markers [J]. Chinese Journal of Tropical Crops, 2019, 40(2): 300−307.(in Chinese)
[9]	黄丽芳, 董云萍, 王晓阳, 等. 利用RAPD标记分析咖啡种质资源的遗传多样性 [J]. 热带作物学报, 2014, 35(12):2313−2319. doi: 10.3969/j.issn.1000-2561.2014.12.001 HUANG L F, DONG Y P, WANG X Y, et al. Genetic diversity analysis of coffee germplasms by RAPD markers [J]. Chinese Journal of Tropical Crops, 2014, 35(12): 2313−2319.(in Chinese) doi: 10.3969/j.issn.1000-2561.2014.12.001
[10]	黄丽芳, 董云萍, 王晓阳, 等. 云南咖啡资源遗传多样性的RAPD分析 [J]. 中国热带农业, 2017(5):48−52. doi: 10.3969/j.issn.1673-0658.2017.05.012 HUANG L F, DONG Y P, WANG X Y, et al. Genetic diversity analysis of coffee germlasms from Yunnan by RAPD Markers [J]. China Tropical Agriculture, 2017(5): 48−52.(in Chinese) doi: 10.3969/j.issn.1673-0658.2017.05.012
[11]	陶爱芬, 祁建民, 林培青, 等. 红麻优异种质产量和品质性状主成分聚类分析与综合评价 [J]. 中国农业科学, 2008, 41(9):2859−2867. doi: 10.3864/j.issn.0578-1752.2008.09.039 TAO A F, QI J M, LIN P Q, et al. Cluster analysis and evaluation of elite kanaf germplasm based on principal components [J]. Scientia Agricultura Sinica, 2008, 41(9): 2859−2867.(in Chinese) doi: 10.3864/j.issn.0578-1752.2008.09.039
[12]	廖丽, 陈玉华, 赵亚荣, 等. 地毯草种质资源形态多样性 [J]. 草业科学, 2015, 32(2):248−257. doi: 10.11829/j.issn.1001-0629.2014-0223 LIAO L, CHEN Y H, ZHAO Y R, et al. Morphology diversity of Axonopus compressus germplasm [J]. Pratacultural Science, 2015, 32(2): 248−257.(in Chinese) doi: 10.11829/j.issn.1001-0629.2014-0223
[13]	魏忠芬, 李慧琳, 奉斌, 等. 贵州紫苏种质资源表型性状的遗传多样性 [J]. 西南农业学报, 2017, 30(1):45−52. WEI Z F, LI H L, FENG B, et al. Genetic Diversity of Phenotype Characters of Perilla frutescens Germplasm Resources in Guizhou [J]. Southwest China Journal of Agricultural Sciences, 2017, 30(1): 45−52.(in Chinese)
[14]	万述伟, 宋风景, 郝俊杰, 等. 271份豌豆种质资源农艺性状遗传多样性分析 [J]. 植物遗传资源学报, 2017, 18(1):10−18. WANG S W, SONG F J, HAO J J, et al. Genetic Diversity of Agronomic Traits in 271 Pea Germplasm Resources [J]. Journal of Plant Genetic Resources, 2017, 18(1): 10−18.(in Chinese)
[15]	代攀虹, 孙君灵, 何守朴, 等. 陆地棉核心种质表型性状遗传多样性分析及综合评价 [J]. 中国农业科学, 2016, 49(19):3694−3708. doi: 10.3864/j.issn.0578-1752.2016.19.003 DAI P H, SUN J L, HE S P, et al. Comprehensive evaluation and genetic diversity analysis of phenotypic traits of core collection in upland cotton [J]. Scientia Agricultura Sinica, 2016, 49(19): 3694−3708.(in Chinese) doi: 10.3864/j.issn.0578-1752.2016.19.003
[16]	KISUA J, MWIKAMBA K, MAKOBE M, et al. Genetic diversity of sweet and grain sorghum populations using phenotypic markers [J]. International Journal of Biosciences, 2015, 6(9): 34−46. doi: 10.12692/ijb/6.9.34-46
[17]	王黎明, 焦少杰, 姜艳喜, 等. 不同来源甜高粱种质资源的表型遗传多样性分析 [J]. 植物遗传资源学报, 2014, 15(2):411−416. WANG L M, JIAO S J, JIANG Y X, et al. Genetic diversity analysis on sweet Sorghum germplasm resources of different origins based on agronomical traits Genetic diversity analysis of sweet Sorghum germplasm resources from different origins using agronomical traits Genetic diversity analysis of sweet Sorghum germplasm resources from different origins using agronomical traits [J]. Journal of Plant Genetic Resources, 2014, 15(2): 411−416.(in Chinese)
[18]	黄家雄, 吕玉兰, 程金焕, 等. 不同海拔对小粒种咖啡品质影响的研究 [J]. 热带农业科学, 2012, 32(8):4−7. doi: 10.3969/j.issn.1009-2196.2012.08.002 HUANG J X, LV Y L, CHENG J H, et al. Preliminary study on the influence of different altitudes on the quality of coffee Arabica [J]. Chinese Journal of Tropical Agriculture, 2012, 32(8): 4−7.(in Chinese) doi: 10.3969/j.issn.1009-2196.2012.08.002
[19]	王光路, 李长文, 刘志达, 等. HPLC法同时测定绿咖啡豆中绿原酸及咖啡碱含量 [J]. 食品研究与开发, 2017, 38(23):155−159. doi: 10.3969/j.issn.1005-6521.2017.23.030 WANG G L, LI C W, LIU Z D, et al. Simultaneous quantitative analysis of chlorogenic acid and caffeine in raw coffee beans by HPLC [J]. Food Research and Development, 2017, 38(23): 155−159.(in Chinese) doi: 10.3969/j.issn.1005-6521.2017.23.030
[20]	高贤玉, 张发明, 柏天琦, 等. 莲雾果实糖酸含量分析 [J]. 热带农业科学, 2019, 39(3):75−79, 90. GAO X Y, ZHANG F M, BAI T Q, et al. Sugar and Organic Acid Contents in the Fruit of Wax Apple [J]. Chinese Journal of tropical agriculture, 2019, 39(3): 75−79, 90.(in Chinese)
[21]	谢向誉, 陆柳英, 曾文丹, 等. 31份木薯种质资源的鉴定评价及遗传多样性分析 [J]. 南方农业学报, 2017, 48(3):393−400. XIE X Y, LU L Y, ZENG W D, et al. Identification, evaluation and genetic diversity analysis of 31 cassava germplasm resources [J]. Journal of Southern Agriculture, 2017, 48(3): 393−400.(in Chinese)
[22]	朱慧珺, 张耀文, 赵雪英, 等. 山西省绿豆种质资源的遗传多样性分析 [J]. 山西农业科学, 2019, 47(9):1540−1543, 1602. doi: 10.3969/j.issn.1002-2481.2019.09.11 ZHU H J, ZHANG Y W, ZHAO X Y, et al. Genetic diversity analysis of mung bean germplasm resources in Shanxi Province [J]. Journal of Shanxi Agricultural Sciences, 2019, 47(9): 1540−1543, 1602.(in Chinese) doi: 10.3969/j.issn.1002-2481.2019.09.11
[23]	马克平. 试论生物多样性的概念 [J]. 生物多样性, 1993, 1(1):20−22. doi: 10.3321/j.issn:1005-0094.1993.01.006 MA K P. The concept of biological diversity [J]. Chinese Biodiversity, 1993, 1(1): 20−22.(in Chinese) doi: 10.3321/j.issn:1005-0094.1993.01.006
[24]	时圣明, 潘明佳, 王洁, 等. 分子鉴定技术在中药中的应用 [J]. 中草药, 2016, 47(17):3121−3126. doi: 10.7501/j.issn.0253-2670.2016.17.027 SHI S M, PAN M J, WANG J, et al. Application of molecular identification techniques in Chinese materia Medica [J]. Chinese Traditional and Herbal Drugs, 2016, 47(17): 3121−3126.(in Chinese) doi: 10.7501/j.issn.0253-2670.2016.17.027
[25]	刘同金, 张晓辉, 沈镝, 等. 欧洲山芥种质资源的表型遗传多样性分析 [J]. 植物遗传资源学报, 2015, 16(3):528−534. LIU T J, ZHANG X H, SHEN D, et al. Analysis on genetic diversity of Barbarea vulgaris germplasm resources based on phenotypic traits [J]. Journal of Plant Genetic Resources, 2015, 16(3): 528−534.(in Chinese)
[26]	聂石辉, 彭琳, 王仙, 等. 鹰嘴豆种质资源农艺性状遗传多样性分析 [J]. 植物遗传资源学报, 2015, 16(1):64−70. NIE S H, PENG L, WANG X, et al. Genetic diversity of agronomic traits in chickpea(Cicer arietinum L.) germplasm resources [J]. Journal of Plant Genetic Resources, 2015, 16(1): 64−70.(in Chinese)
[27]	王晓阳, 黄丽芳, 闫林, 等. 基于SSR标记的我国咖啡种质资源遗传多样性分析及指纹图谱构建 [J]. 热带农业科学, 2018, 38(12):30−36, 42. WANG X Y, HUANG L F, YAN L, et al. Genetic diversity analysis and fingerprinting of coffee germplasm in China by SSR markers [J]. Chinese Journal of Tropical Agriculture, 2018, 38(12): 30−36, 42.(in Chinese)
[28]	曾潮武, 梁晓东, 李建疆. 新疆春小麦种质资源主要农艺性状的遗传多样性分析 [J]. 分子植物育种, 2017, 15(9):3740−3750. ZENG C W, LIANG X D, LI J J. Genetic diversity analysis in main characters of spring wheat germplasm in Xinjiang [J]. Molecular Plant Breeding, 2017, 15(9): 3740−3750.(in Chinese)
[29]	潘存祥, 许勇, 纪海波, 等. 西瓜种质资源表型多样性及聚类分析 [J]. 植物遗传资源学报, 2015, 16(1):59−63. PAN C X, XU Y, JI H B, et al. Phenotypic diversity and clustering analysis of watermelon germplasm [J]. Journal of Plant Genetic Resources, 2015, 16(1): 59−63.(in Chinese)