Advances on Diagnosis and Prevention of <i>Mycoplasma ovipneumoniae</i> Infection on Sheep and Goats

JIANG Jin-xiu; LIN Yu-sheng; ZHANG Jing-peng; YOU Wei; HUANG Li-li; HU Qi-lin

doi:10.19303/j.issn.1008-0384.2019.12.0015

Volume 34 Issue 12

Dec. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(12): 1463-1470

JIANG J X, LIN Y S, ZHANG J P, et al. Advances on Diagnosis and Prevention of Mycoplasma ovipneumoniae Infection on Sheep and Goats [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1463−1470. doi: 10.19303/j.issn.1008-0384.2019.12.0015

Citation:

JIANG J X, LIN Y S, ZHANG J P, et al. Advances on Diagnosis and Prevention of Mycoplasma ovipneumoniae Infection on Sheep and Goats [J]. Fujian Journal of Agricultural Sciences，2019，34（12）：1463−1470. doi: 10.19303/j.issn.1008-0384.2019.12.0015

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Advances on Diagnosis and Prevention of Mycoplasma ovipneumoniae Infection on Sheep and Goats

doi: 10.19303/j.issn.1008-0384.2019.12.0015

1.
Institute of Animal Husbandry & Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Fujian Normal University, Fuzhou, Fujian　350108, China

Received Date: 2018-10-17
Rev Recd Date: 2019-10-20
Publish Date: 2019-12-01

Abstract

Abstract

Mycoplasma ovipneumoniae (Mo) is one of the main pathogens that causes mycoplasmal pneumonia in sheep and goats. The worldwide disease brings great economic loss to the industry. Currently, 3 detecting methods are commonly used for the disease. One applies the pathogen isolation and identification, but the isolation rates tend to be low. The second is the most popular molecular biology method. It employs PCR, LAMP, rapid touchdown PCR-lateral flow assay, or DNA probe technology for the diagnosis. The third one is the immunological detection by indirect hemagglutination, ELISA, immunohistochemistry or indirect immunofluorescence. The disease control largely relies on the vaccination and drug treatment. At present, inactivated vaccines are available, and various genetically engineered vaccines are being developed. The drug treatments of the infection include the uses of antibiotics, traditional Chinese medicine, and combination of traditional Chinese and western medicines. In recent years, the immune defense molecules were reported to show immunomodulatory and therapeutic effects on mycoplasmal pneumonia in sheep and goats. It is postulated to be a new approach for treating the disease. This article reviews the epidemiology, diagnosis, treatments, and research on vaccines of Mo infection.
- Mycoplasma ovipneumoniae infection,
- epidemiology,
- diagnosis,
- treatments,
- vaccine

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References(63)

References

[1]	吴移谋, 叶元康. 支原体学[M]. 第2版. 北京: 人民出版社, 2008: 237-246.
[2]	S AZIZI, E TAJBAKHSH, A REZAⅡ, et al. The role of Mycoplasma ovipneumoniae and Mycoplasma arginini in pneumonic lungs of slaughtered sheep [J]. Revue de Medecine Veterinaire, 2011, 162(6): 310−315.
[3]	王建昌. 绵羊肺炎支原体山东株的分离鉴定和标准株外膜蛋白特性研究[D]. 泰安: 山东农业大学, 2007. WANG J C. Isolation and identification of Mycoplasma ovipneumoniae from Shandong and studies on out membrane protein of standard strain Y-98[D]. Taian: Shandong Agricultural University, 2007.(in Chinese)
[4]	YANG F, TANG C, WANG Y, et al. Genome sequence of Mycoplasma ovipneumoniae strain SC01 [J]. Journal of Bacteriology, 2011, 193(18): 5018. doi: 10.1128/JB.05363-11
[5]	张斯民. 绵羊肺炎支原体甘油-3-磷酸氧化酶基因的克隆、表达及重组产物的纯化[D]. 银川: 宁夏大学, 2013. ZHANG S M. The cloning and expression of glycerol-3-phosphate oxidase of Mycoplasma ovipneumoniae and the purification of it's recombinant product[D]. Yinchuan: Ningxia University, 2013.(in Chinese)
[6]	许健, 储岳峰, 赵萍, 等. 中国部分地区绵羊肺炎支原体的基因多态性分析 [J]. 畜牧兽医学报, 2011, 42(9):1295−1301. XU J, CHU Y F, ZHAO P, et al. Investigation of gene polymorphism on Mycoplasma ovipneumoniae isolates from some areas of China [J]. Chinese Journal of Animal and Veterinary Sciences, 2011, 42(9): 1295−1301.(in Chinese)
[7]	许健, 储岳峰, 高鹏程, 等. 我国17株绵羊肺炎支原体分子分型及其菌体蛋白免疫印迹 [J]. 微生物学报, 2011, 51(10):1421−1426. XU J, CHU Y F, GAO P C, et al. Molecular typing and immunoblotting of 17 Mycoplasma ovipneumoniae isolates from China [J]. Acta Microbiologica Sinica, 2011, 51(10): 1421−1426.(in Chinese)
[8]	JIANG Z J, SONG F Y, LI Y N, et al. Capsular polysaccharide is a main component of Mycoplasma ovipneumoniae in the pathogen-induced toll-like receptor-mediated inflammatory responses in sheep airway epithelial cells [J]. Mediators of Inflammation, 2017, 2017: 1−15.
[9]	江锦秀, 林裕胜, 游伟, 等. 福建省羊支原体性肺炎分子流行病学研究 [J]. 福建农业学报, 2017, 32(1):12−16. JIANG J X, LIN Y S, YOU W, et al. A molecular epidemiological study on Mycoplasma pneumonia of sheep and goats in Fujian [J]. Fujian Journal of Agricultural Sciences, 2017, 32(1): 12−16.(in Chinese)
[10]	吴锦艳, 尚佑军, 陈妍, 等. 国内部分地区羊支原体肺炎血清学调查及分析 [J]. 中国预防兽医学报, 2017, 39(2):156−158. WU J Y, SHANG Y J, CHEN Y, et al. Serological investigation of Mycoplasmal pneumonia of sheep and goats in China [J]. Chinese Journal of Preventive Veterinary Medicine, 2017, 39(2): 156−158.(in Chinese)
[11]	王华, 杨发龙, 王永, 等. 山羊支原体性肺炎流行病学调查 [J]. 中国畜牧兽医, 2011, 38(1):210−214. WANG H, YANG F L, WANG Y, et al. Epidemiological investigation of caprine Mycoplasma pneumoniae in Sichuan Province [J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(1): 210−214.(in Chinese)
[12]	杨明爽. 浙南山区山羊传染性胸膜肺炎流行病学调查与防治 [J]. 中国兽医杂志, 2011, 47(5):37−38. doi: 10.3969/j.issn.0529-6005.2011.05.015 YANG M S. Epidemiological investigation and prevention of caprine infectious pleuropneumonia in mountainous areas of southern Zhejiang [J]. Chinese Journal of Veterinary Medicine, 2011, 47(5): 37−38.(in Chinese) doi: 10.3969/j.issn.0529-6005.2011.05.015
[13]	解慧梅, 苏志国, 任宝波. 泰州地区羊支原体肺炎流行病学调查 [J]. 江苏农业科学, 2015, 43(3):194−195. XIE H M, SU Z G, REN B B. Epidemiological survey of Mycoplasma pneumoniae in goat and sheep in Taizhou area [J]. Jiangsu Agricultural Sciences, 2015, 43(3): 194−195.(in Chinese)
[14]	CHEN C, QIAO J, MENG Q L, et al. Serological and molecular survey of sheep infected with Mycoplasma ovipneumoniae in Xinjiang, China [J]. Tropical Animal Health and Production, 2015, 47(8): 1641−1647. doi: 10.1007/s11250-015-0908-2
[15]	RONG G, ZHAO J M, HOU G Y, et al. Seroprevalence and molecular detection of Mycoplasma ovipneumoniae in goats in tropical China [J]. Tropical Animal Health and Production, 2014, 46(8): 1491−1495. doi: 10.1007/s11250-014-0645-y
[16]	储岳峰. 我国山羊(接触)传染性胸膜肺炎病原学、流行病学研究及灭活疫苗的研制[D]. 北京: 中国农业科学院, 2011. CHU Y F. An etiological and epidemiological study on contagious caprine pleuropneumoniae in China and development of a bacterin against it[D]. Beijing: Chinese Academy of Agricultural Sciences, 2011.(in Chinese)
[17]	许健, 储岳峰, 赵萍, 等. 绵羊肺炎支原体GH3-3株在改良KM2培养基中的增殖情况 [J]. 动物医学进展, 2011, 32(11):46−48. doi: 10.3969/j.issn.1007-5038.2011.11.010 XU J, CHU Y F, ZHAO P, et al. Proliferation of Mycoplasma ovipneumoniae GH3-3 strainin modified KM2 medium [J]. Progress in Veterinary Medicine, 2011, 32(11): 46−48.(in Chinese) doi: 10.3969/j.issn.1007-5038.2011.11.010
[18]	史晓娜, 王晓晖, 高媛, 等. 基于转录组测序结果对绵羊肺炎支原体培养基优化的初步研究 [J]. 中国兽医科学, 2018, 48(1):62−68. SHI X N, WANG X H, GAO Y, et al. Optimization of Mycoplasma ovipneumoniae medium based on transcriptome analysis [J]. Chinese Veterinary Science, 2018, 48(1): 62−68.(in Chinese)
[19]	张双翔, 程振涛, 周碧君, 等. 应用荧光定量PCR筛选绵羊肺炎支原体最适生长培养基 [J]. 畜牧与兽医, 2013, 45(1):9−14. ZHANG S X, CHENG Z T, ZHOU B J, et al. Application of fluorescent quantitative PCR in selecting the best growth medium of Mycoplasma ovipneumoniae [J]. Animal Husbandry & Veterinary Medicine, 2013, 45(1): 9−14.(in Chinese)
[20]	张玲, 郝永清, 黄海碧, 等. 内蒙古地区绵羊肺炎支原体的分离鉴定及序列分析 [J]. 中国畜牧兽医, 2013, 40(7):158−161. doi: 10.3969/j.issn.1671-7236.2013.07.037 ZHANG L, HAO Y Q, HUANG H B, et al. Isolation, identification and sequence analysis of Mycoplasma ovipneumoniae in Inner Mongolia [J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(7): 158−161.(in Chinese) doi: 10.3969/j.issn.1671-7236.2013.07.037
[21]	KILIC A, KALENDER H, EROKSUZ H, et al. Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey [J]. Tropical Animal Health and Production, 2013, 45(7): 1525−1531. doi: 10.1007/s11250-013-0394-3
[22]	YANG F L, HAN X, TANG C, et al. A hsp70 Gene-Based PCR for Detection of Mycoplasma ovipneumoniae [J]. Journal of animal and veterinary advances, 2013, 12(19): 1495−1497.
[23]	储岳峰, 高鹏程, 赵萍, 等. 应用双重PCR方法检测羊支原体肺炎病原 [J]. 畜牧与兽医, 2009, 41(12):23−26. CHU Y F, GAO P C, ZHAO P, et al. Development of a duplex polymerase chain reaction assay for the detection of pathogens for Mycoplasmal pneumonia of sheep and goats [J]. Animal Husbandry & Veterinary Medicine, 2009, 41(12): 23−26.(in Chinese)
[24]	林裕胜, 江锦秀, 江斌, 等. 绵羊肺炎支原体、丝状支原体山羊亚种和山羊支原体山羊肺炎亚种多重PCR检测方法的建立 [J]. 中国预防兽医学报, 2018, 40(9):812−817. LIN Y S, JIANG J X, JIANG B, et al. Development of a triplex PCR assay for detection of Mycoplasma ovipneumoniae, M.mycoides subsp. Capri and M.capricolum subsp. capripneumoniae [J]. Chinese Journal of Preventive Veterinary Medicine, 2018, 40(9): 812−817.(in Chinese)
[25]	林裕胜, 江锦秀, 张靖鹏, 等. 绵羊肺炎支原体TaqMan荧光定量PCR检测方法的建立 [J]. 中国预防兽医学报, 2018, 40(4):316−320. LIN Y S, JIANG J X, ZHANG J P, et al. Establishment of a TaqMan real-time fluorescent quantitative PCR assay for detection of Mycoplasma ovipneumoniae [J]. Chinese Journal of Preventive Veterinary Medicine, 2018, 40(4): 316−320.(in Chinese)
[26]	YANG F L, DAO X F, RODRIGUEZ-PALACIOS A, et al. A real-time PCR for detection and quantification of Mycoplasma ovipneumoniae [J]. Journal of Veterinary Medical Science, 2014, 76(12): 1631−1634. doi: 10.1292/jvms.14-0094
[27]	张双翔, 周碧君, 程振涛, 等. 绵羊肺炎支原体LAMP检测方法的建立及初步应用 [J]. 中国兽医学报, 2013, 33(3):362−366. ZHANG S X, ZHOU B J, CHENG Z T, et al. Development and application of a loop-mediated isothermal amplification assay for detection of Mycoplasma ovipneumoniae [J]. Chinese Journal of Veterinary Science, 2013, 33(3): 362−366.(in Chinese)
[28]	张洁, 曹军军, 祝明松, 等. 绵羊肺炎支原体环介导等温扩增联合横向流动试纸条可视化检测方法的建立 [J]. 动物医学进展, 2019, 40(3):1−8. ZHANG J, CAO J J, ZHU M S, et al. Establishment of loop-mediated isothermal amplification-lateral-flow dipstick for detecting Mycoplasma ovipneumoniae [J]. Progress in Veterinary Medicine, 2019, 40(3): 1−8.(in Chinese)
[29]	ZHANG J, CAO J J, ZHU M S, et al. Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Mycoplasma ovipneumoniae [J]. World Journal of Microbiology and Biotechnology, 2019, 35(2): 31. doi: 10.1007/s11274-019-2601-5
[30]	杜鲜, 乔军, 陈诚, 等. 绵羊肺炎支原体降落PCR-侧向层析快速检测方法的建立 [J]. 中国预防兽医学报, 2017, 39(6):466−470. DU X, QIAO J, CHEN C, et al. Visualized detection of Mycoplasma ovipneumoniae by real-time touchdown PCR-lateral flow assay [J]. Chinese Journal of Preventive Veterinary Medicine, 2017, 39(6): 466−470.(in Chinese)
[31]	CHEN Q W, ZHANG L, JIANG F, et al. MnO₂ microsphere absorbing Cy5-labeled single strand DNA probe serving as powerful biosensor for effective detection of Mycoplasma ovipneumoniae [J]. Sensors and Actuators B: Chemical, 2017, 244: 1138−1144. doi: 10.1016/j.snb.2017.01.104
[32]	赵萍, 逯忠新, 储岳峰, 等. 绵羊肺炎支原体间接血凝诊断方法的建立 [J]. 甘肃农业大学学报, 2008, 43(1):29−32. doi: 10.3969/j.issn.1003-4315.2008.01.006 ZHAO P, LU Z X, CHU Y F, et al. Development of the indirect haemagglutination test of M.ovipneumoniae [J]. Journal of Gansu Agricultural University, 2008, 43(1): 29−32.(in Chinese) doi: 10.3969/j.issn.1003-4315.2008.01.006
[33]	候相山, 王建昌, 张永宁, 等. 羊群绵羊肺炎支原体抗体间接ELISA检测方法的建立 [J]. 中国畜牧兽医, 2006, 33(6):76−78. HOU X S, WANG J C, ZHANG Y N, et al. The establishment of the indirect ELISA for the detection of the antibody against Mycoplasma ovine pneumoniae(MoP) in the sheep and goats [J]. China Animal Husbandry & Veterinary Medicine, 2006, 33(6): 76−78.(in Chinese)
[34]	赵萍, 储岳峰, 高鹏程, 等. 绵羊肺炎支原体间接ELISA检测方法的建立及应用 [J]. 福建农林大学学报(自然科学版), 2008, 37(5):510−513. ZHAO P, CHU Y F, GAO P C, et al. Establishment and application of indirect ELISA method for Mycoplasma ovipneumoniae [J]. Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 2008, 37(5): 510−513.(in Chinese)
[35]	黄海碧, 郑佳琪, 王仁超, 等. 绵羊肺炎支原体P71蛋白分段表达及其间接ELISA方法的建立 [J]. 中国预防兽医学报, 2015, 37(8):623−627. doi: 10.3969/j.issn.1008-0589.2015.08.13 HUANG H B, ZHENG J Q, WANG R C, et al. Development of an indirect ELISA method with the main antigentic region of Mycoplasma ovipneumoniae P71 protein expressed in E.coli [J]. Chinese Journal of Preventive Veterinary Medicine, 2015, 37(8): 623−627.(in Chinese) doi: 10.3969/j.issn.1008-0589.2015.08.13
[36]	程振涛, 王慧, 王开功, 等. 一种基于Mo Hsp70蛋白的间接ELISA试剂盒及使用方法: CN104597249A[P]. 2015-05-06.
[37]	张建华, 黄海碧, 王晓晖, 等. 绵羊肺炎支原体免疫组织化学及间接免疫荧光检测方法的建立 [J]. 中国兽医科学, 2017, 47(2):172−178. ZHANG J H, HUANG H B, WANG X H, et al. Establishment of indirect immunofluorescence assay and immunohistochemistry assay for detection of Mycoplasma ovipneumonia [J]. Chinese Veterinary Science, 2017, 47(2): 172−178.(in Chinese)
[38]	韩笑, 岳华, 王远微, 等. 绵羊肺炎支原体灭活疫苗的研制 [J]. 中国畜牧兽医, 2011, 38(11):181−184. HAN X, YUE H, WANG Y W, et al. Development of inactivated Mycoplasma ovipneumoniae vaccine [J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(11): 181−184.(in Chinese)
[39]	逯忠新, 储岳峰, 赵萍, 等. 山羊支原体肺炎二联灭活疫苗及其制备方法: CN101721694A[P]. 2010-06-09.
[40]	费磊, 岳华, 汤承. 4种佐剂对绵羊肺炎支原体的免疫辅佐效力研究 [J]. 中国畜牧兽医, 2013, 40(3):220−223. doi: 10.3969/j.issn.1671-7236.2013.03.050 FEI L, YUE H, TANG C. Study on the four adjuvant for inactivated vaccine of Mycoplasma ovipneumoniae immune effectiveness [J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(3): 220−223.(in Chinese) doi: 10.3969/j.issn.1671-7236.2013.03.050
[41]	SOMYA MEHRA, RAJNEESH RANA, VIJAY KUMAR, et al. Evaluation of combined adjuvant vis-a-vis single adjuvant for development of caprine pleuropneumonia vaccine in goats using indigenous Mycoplasma mycoides subsp.capri isolate [J]. Indian Journal of Animal Sciences, 2013, 83(5): 473−476.
[42]	MANIMARAN K, SINGH V P, LTU K, et al. Immune responses against different inactivated vaccines of Mycoplasma mycoides subsp.capri in goats [J]. Journal of Applied Animal Research, 2006, 29(2): 117−120. doi: 10.1080/09712119.2006.9706584
[43]	冯旭飞, 刀筱芳, 张贤宇, 等. 绵羊肺炎支原体p128基因序列分析及原核表达 [J]. 中国兽医杂志, 2015, 51(2):20−22, 26. doi: 10.3969/j.issn.0529-6005.2015.02.006 FENG X F, DAO X F, ZHANG X Y, et al. Sequence analysis and prokaryotic expression of Mycoplasma ovipneumoniae p128 gene [J]. Chinese Journal of Veterinary Medicine, 2015, 51(2): 20−22, 26.(in Chinese) doi: 10.3969/j.issn.0529-6005.2015.02.006
[44]	陈诚, 乔军, 孟庆玲, 等. 绵羊肺炎支原体膜蛋白p56基因的克隆、表达及反应原性研究 [J]. 西南农业学报, 2016, 29(2):455−458. CHEN C, QIAO J, MENG Q L, et al. Cloning and expression of membrane protein p56 gene of Mycoplasma ovipneumoniae and its identification of reactogenicity [J]. Southwest China Journal of Agricultural Sciences, 2016, 29(2): 455−458.(in Chinese)
[45]	卢海亭, 孟庆玲, 乔军, 等. 绵羊肺炎支原体膜蛋白p74基因的克隆、分子特征及反应原性研究 [J]. 西南农业学报, 2017, 30(5):1222−1228. LU H T, MENG Q L, QIAO J, et al. Cloning, molecular characteristics and reactogenicity of membrane protein p74 of Mycoplasma ovipneumoniae [J]. Southwest China Journal of Agricultural Sciences, 2017, 30(5): 1222−1228.(in Chinese)
[46]	张贤宇, 杨发龙, 冯旭飞, 等. 绵羊肺炎支原体P113蛋白N端原核表达及其抗原性分析 [J]. 中国兽医杂志, 2013, 49(7):7−10. doi: 10.3969/j.issn.0529-6005.2013.07.002 ZHANG X Y, YANG F L, FENG X F, et al. Prokaryotic expression and antigenicity analysis of N-terminal of Mycoplasma ovipneumoniae P113 protein [J]. Chinese Journal of Veterinary Medicine, 2013, 49(7): 7−10.(in Chinese) doi: 10.3969/j.issn.0529-6005.2013.07.002
[47]	JIANG F, HE J Y, NAVARRO-ALVAREZ N, et al. Elongation factor Tu and heat shock protein 70 are membrane-associated proteins from Mycoplasma ovipneumoniae capable of inducing strong immune response in mice [J]. PLoS One, 2016, 11(8): e0161170. doi: 10.1371/journal.pone.0161170
[48]	MAKSIMOVIĆ Z, DE LA FE C, AMORES J, et al. Comparison of phenotypic and genotypic profiles among caprine and ovine Mycoplasma ovipneumoniae strains [J]. Veterinary Record, 2017, 180(7): 180. doi: 10.1136/vr.103699
[49]	田路路, 孟庆玲, 乔军, 等. 绵羊肺炎支原体多表位融合基因MO-meAg₁的构建、表达及重组蛋白免疫原性分析 [J]. 西北农业学报, 2017, 26(5):678−684. doi: 10.7606/j.issn.1004-1389.2017.05.005 TIAN L L, MENG Q L, QIAO J, et al. Construction, expression and reactionogenicity of multi-epitope fusion gene of Mycoplasma ovipneumoniae [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2017, 26(5): 678−684.(in Chinese) doi: 10.7606/j.issn.1004-1389.2017.05.005
[50]	张轩. 绵羊肺炎支原体多表位疫苗的研究及免疫试验[D]. 北京: 中国农业科学院, 2013. ZHANG X. Research and immune experiments of multi-epitope vaccine of Mycoplasma ovipneumoniae[D]. Beijing: Chinese Academy of Agricultural Sciences, 2013.(in Chinese)
[51]	刘文青, 范琼瑛, 赵红艳, 等. 绵羊肺炎支原体Y₉₈ P26-P40融合基因的表达及免疫原性 [J]. 中国兽医学报, 2013, 33(10):1552−1558. LIU W Q, FAN Q Y, ZHAO H Y, et al. Research on the expression of P26-P40 fusion protein of Mycoplasma ovipneumonia(Y-98) and its immunogenicity [J]. Chinese Journal of Veterinary Science, 2013, 33(10): 1552−1558.(in Chinese)
[52]	卢海亭, 乔军, 孟庆玲, 等. 绵羊肺炎支原体p74基因重组单核细胞增生李斯特菌的构建及其免疫原性分析 [J]. 西北农林科技大学学报(自然科学版), 2017, 45(8):7−14, 22. LU H T, QIAO J, MENG Q L, et al. Construction and immunogenicity of recombinant Listeria monocytogenes expressing Mycoplasma ovipneumoniae p74 gene [J]. Journal of Northwest A & F University(Natural Science Edition), 2017, 45(8): 7−14, 22.(in Chinese)
[53]	宋勤叶, 张英杰, 刘月琴, 等. 18种抗菌药物对绵羊肺炎支原体和丝状支原体分离株的抗菌活性 [J]. 动物医学进展, 2011, 32(6):14−18. doi: 10.3969/j.issn.1007-5038.2011.06.004 SONG Q Y, ZHANG Y J, LIU Y Q, et al. Antimicrobial activities of eighteen antimicrobials against Mycoplasma ovipneumoniae and Mycoplasma mycoides isolates in vitro [J]. Progress in Veterinary Medicine, 2011, 32(6): 14−18.(in Chinese) doi: 10.3969/j.issn.1007-5038.2011.06.004
[54]	陈朝喜, 王华, 朱晓霞, 等. 山羊源绵羊肺炎支原体的耐药谱型分析 [J]. 中国畜牧兽医, 2011, 38(1):179−182. CHEN C X, WANG H, ZHU X X, et al. Drug resistance pattern analysis of Mycoplasma ovipneumoniae isolated from goat [J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(1): 179−182.(in Chinese)
[55]	PATERNA A, TATAY-DUALDE J, AMORES J, et al. In vitro assessment of the antimicrobial susceptibility of caprine isolates of Mycoplasma mycoides subsp. Capri [J]. The Veterinary Journal, 2016, 214: 96−101. doi: 10.1016/j.tvjl.2016.05.010
[56]	TATAY-DUALDE J, PRATS-VAN DER HAM MP V, DE LA FE C, et al. Resistance mechanisms against quinolones in Mycoplasma capricolum subsp. capricolum [J]. The Veterinary Journal, 2017, 223: 1−4. doi: 10.1016/j.tvjl.2017.03.007
[57]	GAUTIER-BOUCHARDON A V, FERRÉ S, LE GRAND D, et al. Overall decrease in the susceptibility of Mycoplasma bovis to antimicrobials over the past 30 years in France [J]. PLoS One, 2014, 9(2): e87672. doi: 10.1371/journal.pone.0087672
[58]	陈美安, 甄汉深, 裴渭静, 等. 10味中药对畜禽支原体敏感性及细胞毒性研究 [J]. 家畜生态学报, 2014, 35(2):58−63. doi: 10.3969/j.issn.1673-1182.2014.02.012 CHEN M A, ZHEN H S, PEI W J, et al. Research on the livestock and poultry MycoplasmaSensitivity and cytotoxicity of ten kinds of traditional Chinese medicine in vitro [J]. Acta Ecologae Animalis Domastici, 2014, 35(2): 58−63.(in Chinese) doi: 10.3969/j.issn.1673-1182.2014.02.012
[59]	温伟, 李晓慧, 王玉福. 麻杏石甘汤加味配合西药治疗绵羊支原体肺炎 [J]. 中国兽医杂志, 2013, 49(1):85−86. doi: 10.3969/j.issn.0529-6005.2013.01.037 WEN W, LI X H, WANG Y F. Ma Xing Shi Gan Decoction Combined with Western Medicine for the Treatment of Mycoplasma ovipneumoniae [J]. Chinese Journal of Veterinary Medicine, 2013, 49(1): 85−86.(in Chinese) doi: 10.3969/j.issn.0529-6005.2013.01.037
[60]	刘海燕, 王继雪, 赵宁, 等. 重组SPLUNC1蛋白对感染绵羊肺炎支原体的盘羊杂交羊的免疫调节作用研究 [J]. 中国预防兽医学报, 2018, 40(5):426−430. LIU H Y, WANG J X, ZHAO N, et al. The immune regulation effect of the recombinant splunc1 protein in argali hybrid sheep infected with Mycoplasma ovipneumoniae [J]. Chinese Journal of Preventive Veterinary Medicine, 2018, 40(5): 426−430.(in Chinese)
[61]	郭海英, 沈文, 陈冬梅, 等. BPI蛋白对感染绵羊肺炎支原体的盘羊杂交羊细胞因子水平的影响 [J]. 畜牧兽医学报, 2015, 46(10):1882−1890. doi: 10.11843/j.issn.0366-6964.2015.10.023 GUO H Y, SHEN W, CHEN D M, et al. The influence of BPI protein on cytokines level in argali hybrid sheep infected with Mycoplasma ovipneumoniae [J]. Chinese Journal of Animal and Veterinary Sciences, 2015, 46(10): 1882−1890.(in Chinese) doi: 10.11843/j.issn.0366-6964.2015.10.023
[62]	姜方配, 沈文, 鲁海富, 等. ISG15蛋白对感染绵羊肺炎支原体的盘羊杂交羊的免疫调节作用 [J]. 中国预防兽医学报, 2013, 35(11):925−928. doi: 10.3969/j.issn.1008-0589.2013.11.15 JIANG F P, SHEN W, LU H F, et al. The immune regulation effect of recombinant ISG15 protein on the argali hybrid sheep infected with the Mycoplasma ovipneumoniae [J]. Chinese Journal of Preventive Veterinary Medicine, 2013, 35(11): 925−928.(in Chinese) doi: 10.3969/j.issn.1008-0589.2013.11.15
[63]	翟梦婷, 赵宗胜, 梁艳萍, 等. 注射甘露糖结合凝集素治疗人工感染绵羊肺炎支原体的效果 [J]. 畜牧与兽医, 2019, 51(3):118−122. ZHAI M T, ZHAO Z S, LIANG Y P, et al. Effect of mannose binding lectin on experimental infection with Mycoplasma ovipneumoniae in sheep [J]. Animal Husbandry & Veterinary Medicine, 2019, 51(3): 118−122.(in Chinese)