Pathogens of Diseases on Jianyang Tangelos

GE Ci-bin; HUANG Su-fang; RUAN Chuan-qing; XIAO Rong-feng; LIU Tao

doi:10.19303/j.issn.1008-0384.2019.08.014

Volume 34 Issue 8

Aug. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(8): 958-964

GE Ci-bin, HUANG Su-fang, RUAN Chuan-qing, XIAO Rong-feng, LIU Tao. Pathogens of Diseases on Jianyang Tangelos[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 958-964. doi: 10.19303/j.issn.1008-0384.2019.08.014

Citation:

GE Ci-bin, HUANG Su-fang, RUAN Chuan-qing, XIAO Rong-feng, LIU Tao. Pathogens of Diseases on Jianyang Tangelos[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 958-964. doi: 10.19303/j.issn.1008-0384.2019.08.014

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Pathogens of Diseases on Jianyang Tangelos

doi: 10.19303/j.issn.1008-0384.2019.08.014

1.
Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
2.
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2019-07-15
Rev Recd Date: 2019-08-24
Publish Date: 2019-08-01

Abstract

Abstract

Objective To identify the pathogens of the severe defoliation and dieback diseases on Jianyang tangelos. Method Leaf specimens were collected from the diseased tangelo plants in Jianyang city. Microbial strains were isolated for morphological observation, pathogenicity determination, and sequence analysis on internal transcribed spacer (ITS) of ribosomal DNA for taxonomic identification. Result Separately, two strains were considered the pathogens for the diseases. The FJAT-32214 strain formed grayish brown colonies on a PSA medium and produced brown septate spores. Its rDNA-ITS sequences showed a 99% similarity with that of Alternaria alternata from NCBI. The other strain, FJAT-32151, formed on PSA gray-white, velvety, dense colonies with brick-red spore piles on the surface in late stage and produced columnar or rod-like, unicellular, colorless spores. The rDNA-ITS sequence of FJAT-32151 was 99% homologous with that of Colletotrichum gloeosporioides. Each of these isolated strains met Koch requirement with a challenge test by inoculation on healthy plants to show the typical morphological characteristics of the respective fungal disease. Conclusion It was confirmed that A. alternata was the pathogen that caused the brown spots disease, and C. gloeosporioides the anthracnose disease on the tangelos.
- Jianyang tangelo,
- disease diagnosis,
- Alternaria alternata,
- Colletotrichum gloeosporioides

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References(27)

References

[1]	刘韬, 吴瑞东.杂柑类新品种——建阳橘柚的选育[J].果树学报, 2007, 20(2):250-251. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx200702027 LIU T, WU R D. Jianyang Tangelo, a new citrus hybrid cultivar[J]. Journal of Fruit Science, 2007, 20(2):250-251. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx200702027
[2]	陈明华, 杨福良. "建阳桔柚"产业现状、存在问题与应对措施[J].农业科技通讯, 2017(2):23-25. doi: 10.3969/j.issn.1000-6400.2017.02.009 CHEN M H, YANG F L. Current situation, existing problems and countermeasures of Jianyang Tangelo[J]. Bulletin of Agricultural Science and Technology, 2017(2):23-25. (in Chinese) doi: 10.3969/j.issn.1000-6400.2017.02.009
[3]	叶新.建阳桔柚主要病虫害综合防治技术[J].福建农业, 2015(4):122-123. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fjny201504022 YE X. Integrated control technology of major pests and diseases of Jianyang tangelo[J]. Fujian Agriculture, 2015(4):122-123. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fjny201504022
[4]	刘韬, 吴瑞东, 林文斌.建阳桔柚优质高产栽培关键技术[J].中国农技推广, 2016, 32(11):34-35. doi: 10.3969/j.issn.1002-381X.2016.11.016 LIU T, WU R D, LIN W B. Key techniques for high quality and yield cultivation of Jianyang tangelo[J]. China Agricultural Technology Extension, 2016, 32(11):34-35. (in Chinese) doi: 10.3969/j.issn.1002-381X.2016.11.016
[5]	易龙, 卢占军, 赖晓桦, 等.纽荷尔脐橙对建阳橘柚上柑橘衰退病毒分离株构成的影响[J].农业生物技术学报, 2012, 20(2):129-134. doi: 10.3969/j.issn.1674-7968.2012.02.003 YI L, LU Z J, LAI X H, et al. Influence of Mewhall navel orange on composition of Citrus tristeza virus isolates collected from Jianyang tangelo[J]. Journal of Agricultural Biotechnology, 2012, 20(2):129-134. (in Chinese) doi: 10.3969/j.issn.1674-7968.2012.02.003
[6]	MONDAL S N, DUTT M, GROSSER J W, et al. Transgenic citrus expressing the antimicrobial gene Attacin E (attE) reduces the susceptibility of 'Duncan' grapefruit to the citrus scab caused by Elsino fawcettii[J]. European Journal of Plant Pathology, 2012, 133(2):391-404. doi: 10.1007/s10658-011-9912-1
[7]	王震, 杨媚, 杨迎青.广东省柑橘炭疽病病原菌的形态与分子鉴定[J].菌物学报, 2010, 29(4):488-493. http://d.old.wanfangdata.com.cn/Periodical/jwxt201004005 WANG Z, YANG M, YANG Y Q, et al. Morphological and molecular identification of citrus anthracnose pathogen from Guangdong Province[J]. Mycosystema, 2010, 29(4):488-493. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jwxt201004005
[8]	GUARNACCIA V, CROUS P W. Emerging citrus diseases in Europe caused by species of Diaporthe[J]. IMA Fungus, 2017, 8(2):317-334. doi: 10.5598/imafungus.2017.08.02.07
[9]	ANN P J, KO W H, SU H J. Interaction between Likubin bacterium and Phytophthora parasitica in citrus hosts[J]. European Journal of Plant Pathology, 2004, 110(1):1-6. http://cn.bing.com/academic/profile?id=177c14f86fe7f86fdb3600df57db7fcd&encoded=0&v=paper_preview&mkt=zh-cn
[10]	BONANTS P J M, CARROLL G C, WEERDT M, et al. Development and validation of a fast PCR-based detection method for pathogenic isolates of the citrus black spot fungus, Guignardia citricarpa[J]. European Journal of Plant Pathology, 2003, 109:503-513. doi: 10.1023/A:1024219629669
[11]	REYNOLDS D R. Capnodium citri:The sooty mold fungi comprising the taxon concept[J]. Mycopathologia, 1999, 148:141-147. doi: 10.1023/A:1007170504903
[12]	朱丽, 王兴红, 黄峰, 等.柑橘花瓣灰霉病诱导的果面疤痕研究[J].果树学报, 2012, 29(6):1074-1077. http://www.cnki.com.cn/Article/CJFDTotal-GSKK201206021.htm ZHU L, WANG X H, HUANG F, et al. Investigation of surface defect of Citrus fruits caused by Botrytis-Molded petals[J]. Journal of Fruit Science, 2012, 29(6):1074-1077. (in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-GSKK201206021.htm
[13]	陆家云.植物病害诊断[M].北京:中国农业出版社, 1978:63-227. LU J Y. Diagnosis of Plant Diseases[M]. Beijing:China Agricultural Press, 1978:63-227. (in Chinese)
[14]	方中达.植病研究方法[M].北京:中国农业出版社, 1998. FANG Z D. Research Methods of Plant Diseases[M]. Beijing:China Agricultural Press, 1998. (in Chinese)
[15]	WHITE T J, BURNS T, LEE S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, In:Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols:a guide to methods and applications[M]. New York (NY):Academic Press, 1990:315-322.
[16]	TAMURA K, DUKLEY J, NEI M, et al. Mega4:molecular evolutionary genetics analysis(mega) software version 4.0[J]. Molecular Biology and Evolution, 2007, 24(8):1596-1599. doi: 10.1093/molbev/msm092
[17]	TAMURA K, NEI M, KUMAR S. Prospects for inferring very large phylogenies by using the neighbor-joining method[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(30):11030-11035. doi: 10.1073/pnas.0404206101
[18]	李河, 周国英, 刘君昂.双孢蘑菇褐腐病病原菌的分离及分子鉴定[J].食用菌学报, 2009, 16(2):74-76. doi: 10.3969/j.issn.1005-9873.2009.02.014 LI H, ZHOU G Y, LIU J A. Isolation and molecular identification of a pathogen causing brown rot in Agaricus bisporus[J]. Acta Edulis Fungi, 2009, 16(2):74-76. (in Chinese) doi: 10.3969/j.issn.1005-9873.2009.02.014
[19]	PEEVER T L, SU G, CARPENTER B L, et al. Molecular systematic of citrus-associated Alternaria species[J]. Mycologia, 2004, 96(1):119-134. doi: 10.1080/15572536.2005.11833002
[20]	李红叶, 梅秀凤, 符雨诗, 等.柑橘链格孢褐斑病的发生危害风险和治理对策[J].果树学报, 2015, 32(5):969-976. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx201505029 LI H Y, MEI X F, FU Y S, et al. Alternaria brown spot of citrus:the risk and management strategy[J]. Journal of Fruit Science, 2015, 32(5):969-976. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx201505029
[21]	PEEVER T L, IBANEZ A, AKIMITSU K, et al. Worldwide phylogeography of the citrus brown spot pathogen, Alternaria alternate[J]. Phytopathology, 2002, 92(7):794-802. doi: 10.1094/PHYTO.2002.92.7.794
[22]	WANG X F, LI Z A, TANG K Z, et al. First report of Alternaria brown spot of citrus caused by Alternaria alternata in Yunnan Province, China[J]. Plant Disease, 2010, 94(3):375. http://cn.bing.com/academic/profile?id=9165dba25e0f987e5f2c18784e098e18&encoded=0&v=paper_preview&mkt=zh-cn
[23]	赵圆, 王玲杰, 王雪峰, 等.杂柑褐斑病的病原鉴定[J].果树学报, 2014, 31(2):292-295. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx201402020 ZHAO Y, WANG L J, WANG X F, et al. Identification of the pathogenic fungus causing brown spot in two tangerine hybrid varieties[J]. Journal of Fruit Science, 2014, 31(2):292-295. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx201402020
[24]	韩国兴, 符雨诗, 阮若昕, 等.柑橘链格孢褐斑病病菌对异菌脲和嘧菌环胺敏感性评价[J].中国植保导刊, 2017, 37(7):11-15. doi: 10.3969/j.issn.1672-6820.2017.07.002 HAN G X, FU Y S, RUAN R X, et al. Sensitivity assay of citrus Alter alternata to iprodione and cyprodinil[J]. China Plant Protection, 2017, 37(7):11-15. (in Chinese) doi: 10.3969/j.issn.1672-6820.2017.07.002
[25]	杨媚, 冯淑杰, 何银银, 等.柑橘炭疽病高效杀菌剂的筛选及抗药性菌株的发现[J].华南农业大学学报, 2013, 34(1):28-31, 40. http://d.old.wanfangdata.com.cn/Periodical/hnnydxxb201301006 YANG M, FENG S J, HE Y Y, et al. Screening of efficient fungicides for the control of citrus anthracnose and the discovery of fungicide-resistant isolates[J]. Journal of South China Agricultural University, 2013, 34(1):28-31, 40. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hnnydxxb201301006
[26]	焦雁翔, 王日葵, 胡军华, 等.重庆及周边部分地区柑橘炭疽病菌鉴定[J].果树学报, 2014, 31(5):893-900. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx201405021 JIAO Y X, WANG R K, HU J H, et al. Identification of citrus anthracnose pathogens in Chongqing and some adjacent areas[J]. Journal of Fruit Science, 2014, 31(5):893-900. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gskx201405021
[27]	张玉洁, 张志信, 李红超.柑橘炭疽病的病原鉴定和综合防治措施[J].北方园艺, 2011(2):156-159. http://d.old.wanfangdata.com.cn/Periodical/bfyany201102054 ZHANG Y J, ZHANG Z X, LI H C. Identification and prevention method of pathogenic fungi of citrus anthracnose[J]. Northern Horticulture, 2011(2):156-159. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/bfyany201102054