Cloning and Prokaryotic Expression of NtCCHC in Narcissus tazetta var. chinensis

ZOU Hui; LI Hai-ming; WANG Wei-ying; LIN Jiang-bo

doi:10.19303/j.issn.1008-0384.2019.08.004

Volume 34 Issue 8

Aug. 2019

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ZOU Hui, LI Hai-ming, WANG Wei-ying, LIN Jiang-bo. Cloning and Prokaryotic Expression of NtCCHC in Narcissus tazetta var. chinensis[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 889-893. doi: 10.19303/j.issn.1008-0384.2019.08.004

Citation:

ZOU Hui, LI Hai-ming, WANG Wei-ying, LIN Jiang-bo. Cloning and Prokaryotic Expression of NtCCHC in Narcissus tazetta var. chinensis[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 889-893. doi: 10.19303/j.issn.1008-0384.2019.08.004

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Cloning and Prokaryotic Expression of NtCCHC in Narcissus tazetta var. chinensis

doi: 10.19303/j.issn.1008-0384.2019.08.004

Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, China

Received Date: 2019-03-07
Rev Recd Date: 2019-07-18
Publish Date: 2019-08-01

Abstract

Abstract

Objective Based on the information in the SSH Library on Narcissus tazetta var.chinensis, the zinc finger gene was cloned and prokaryotic expressed for future studies. Method Total RNA of N.tazetta var. chinensis from the paclobutrazol-treated leaves was extracted using the Trizol method. After reverse transcription into cDNA, gene cloning and homology analysis were carried out on it with primers designed according to the known sequence of CCHC. A prokaryotic expression vector was constructed to induce expression. Result The 810 bp sequence encoding 269 amino acids was cloned. The cloned gene showed high homologies with the CCHC genes of many species and was named NtCCHC, which could be successfully induced and expressed in pGEX-4T-3. The phylogenetic tree constructed by MEGA 6.0 indicated that the closest genetic distance of NtCCHC lied with Nelunbo nucifera. Conclusion This study successfully cloned and expressed the NtCCHC gene sequence of N. chinensis.
- Narcissus tazetta var.chinensis,
- zinc finger protein,
- gene cloning,
- protein expression

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References(16)

References

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